PMID- 37163198
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230511
IS  - 1741-427X (Print)
IS  - 1741-4288 (Electronic)
IS  - 1741-427X (Linking)
VI  - 2023
DP  - 2023
TI  - Antidiabetic and Liver Histological and Ultrastructural Effects of Cynara 
      scolymus Leaf and Flower Head Hydroethanolic Extracts in 
      Nicotinamide/Streptozotocin-Induced Diabetic Rats.
PG  - 4223026
LID - 10.1155/2023/4223026 [doi]
LID - 4223026
AB  - This study aims to investigate the effect of hydroethanolic extracts of Cynara 
      scolymus (C. scolymus) leaf (CLHE) and C. scolymus flower (CFHE) on the hepatic 
      histopathological lesions and functional biochemical changes induced by type 2 
      diabetes mellitus (T2DM). The rat model of T2DM was induced by intraperitoneal 
      injection of streptozotocin (STZ) in a dose of 60 mg/kg for 15 minutes following 
      nicotinamide (NA) (60 mg/kg). The rats were allocated into four groups: group 1 
      (negative control), group 2 (diabetic control), group 3 (diabetic rats 
      supplemented with 100 mg/kg/day CLHE), and group 4 (diabetic rats supplemented 
      with 100 mg/kg/day CFHE). Treatment with CLHE and CFHE, for the study duration of 
      28 days, significantly improved the deteriorated hepatic glycogen content, 
      glycogen phosphorylase, glucose-6-phosphatase activities, serum fructosamine 
      levels, lipid profile, aspartate transaminase activities, and alanine 
      transaminase activities as well as serum insulin and C-peptide levels. The 
      elevated liver lipid peroxidation and the decreased activities of superoxide 
      dismutase and glutathione peroxidase were significantly alleviated. The elevated 
      expression of the proinflammatory cytokine tumor necrosis factor-alpha in the liver 
      of diabetic rats was significantly reduced by treatments with CLHE and CFHE. 
      NA/STZ-induced T2DM exhibited hepatic histopathological changes in the form of 
      disordered hepatocytes, cytoplasm dissolution, and mononuclear leukocytic 
      infiltration. The electron microscopic ultrastructure study revealed damaged 
      mitochondria with ill-defined cristae and fragmentation of the rough endoplasmic 
      reticulum. Treatments with CLHE and CFHE remarkably amended these 
      histopathological and EM ultrastructural changes. In conclusion, both CLHE and 
      CFHE may have antidiabetic and improvement effects on the liver function and 
      structural integrity, which may be mediated, at least in part, via suppression of 
      inflammation and oxidative stress and enhancement of the antioxidant defence 
      system.
CI  - Copyright (c) 2023 Osama M. Ahmed et al.
FAU - Ahmed, Osama M
AU  - Ahmed OM
AUID- ORCID: 0000-0003-3781-9709
AD  - Physiology Division, Department of Zoology, Faculty of Science, Beni-Suef 
      University, P.O. 62521, Beni-Suef, Egypt.
FAU - Abdel Fattah, Asmaa A
AU  - Abdel Fattah AA
AUID- ORCID: 0000-0002-7362-6392
AD  - Cell Biology, Histology and Genetics Division, Department of Zoology, Faculty of 
      Science, Beni-Suef University, P.O. 62521, Beni-Suef, Egypt.
FAU - Abdul-Hamid, Manal
AU  - Abdul-Hamid M
AUID- ORCID: 0000-0002-0877-3097
AD  - Cell Biology, Histology and Genetics Division, Department of Zoology, Faculty of 
      Science, Beni-Suef University, P.O. 62521, Beni-Suef, Egypt.
FAU - Abdel-Aziz, Ayman M
AU  - Abdel-Aziz AM
AUID- ORCID: 0000-0001-9476-8923
AD  - Zoology Department, Faculty of Science, Fayoum University, Fayoum 63514, Egypt.
FAU - Sakr, Hader I
AU  - Sakr HI
AUID- ORCID: 0000-0003-2917-2423
AD  - Department of Medical Physiology, Faculty of Medicine, Cairo University, Cairo, 
      Egypt.
AD  - Department of Medical Physiology, Medicine Program, Batterjee Medical College, 
      Jeddah 21442, Saudi Arabia.
FAU - Damanhory, Ahmed A
AU  - Damanhory AA
AUID- ORCID: 0000-0002-3441-7459
AD  - Department of Biochemistry, Faculty of Medicine, Al-Azhar University, Cairo, 
      Egypt.
AD  - Department of Biochemistry, Medicine Program, Batterjee Medical College, Jeddah 
      21442, Saudi Arabia.
FAU - Abdel-Kawi, Samraa H
AU  - Abdel-Kawi SH
AUID- ORCID: 0000-0002-7284-0856
AD  - Department of Medical Histology and Cell Biology, Faculty of Medicine, Beni-Suef 
      University, Beni-Suef, Egypt.
FAU - Ghaboura, Nehmat
AU  - Ghaboura N
AUID- ORCID: 0000-0002-0785-4135
AD  - Department of Pharmacy Practice, Pharmacy Program, Batterjee Medical College, 
      P.O. Box 6231, Jeddah 21442, Saudi Arabia.
FAU - Awad, Moaaz M Y
AU  - Awad MMY
AUID- ORCID: 0000-0001-8572-1672
AD  - Department of Anatomy, Medicine Program, Batterjee Medical College, Jeddah, Saudi 
      Arabia.
AD  - Department of Anatomy, Damietta Faculty of Medicine, Al-Azhar University, 
      Damietta, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20230425
PL  - United States
TA  - Evid Based Complement Alternat Med
JT  - Evidence-based complementary and alternative medicine : eCAM
JID - 101215021
PMC - PMC10164244
COIS- The authors declare that they have no conflicts of interest.
EDAT- 2023/05/10 12:42
MHDA- 2023/05/10 12:43
PMCR- 2023/04/25
CRDT- 2023/05/10 11:26
PHST- 2022/09/12 00:00 [received]
PHST- 2023/02/25 00:00 [revised]
PHST- 2023/04/01 00:00 [accepted]
PHST- 2023/05/10 12:43 [medline]
PHST- 2023/05/10 12:42 [pubmed]
PHST- 2023/05/10 11:26 [entrez]
PHST- 2023/04/25 00:00 [pmc-release]
AID - 10.1155/2023/4223026 [doi]
PST - epublish
SO  - Evid Based Complement Alternat Med. 2023 Apr 25;2023:4223026. doi: 
      10.1155/2023/4223026. eCollection 2023.