PMID- 37163937
OWN - NLM
STAT- MEDLINE
DCOM- 20230529
LR  - 20230531
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 665
DP  - 2023 Jul 12
TI  - FNCaMP, ratiometric green calcium indicator based on mNeonGreen protein.
PG  - 169-177
LID - S0006-291X(23)00545-4 [pii]
LID - 10.1016/j.bbrc.2023.04.108 [doi]
AB  - Neurobiologists widely use green genetically encoded calcium indicators (GECIs) 
      for visualization of neuronal activity. Among them, ratiometric GECIs allow 
      imaging of both active and non-active neuronal populations. However, they are not 
      popular, since their properties are inferior to intensiometric GCaMP series of 
      GECIs. The most characterized and developed ratiometric green GECI is FGCaMP7. 
      However, the dynamic range and sensitivity of its large Stock's shift green 
      (LSS-Green) form is significantly lower than its Green form and its molecular 
      design is not optimal. To address these drawbacks, we engineered a ratiometric 
      green calcium indicator, called FNCaMP, which is based on bright mNeonGreen 
      protein and calmodulin from A. niger and has optimal NTnC-like design. We 
      compared the properties of the FNCaMP and FGCaMP7 indicators in vitro, in 
      mammalian cells, and in neuronal cultures. Finally, we obtained and analyzed 
      X-ray structure of the FNCaMP indicator.
CI  - Copyright (c) 2023 Elsevier Inc. All rights reserved.
FAU - Subach, Oksana M
AU  - Subach OM
AD  - Complex of NBICS Technologies, National Research Center "Kurchatov Institute", 
      Moscow, 123182, Russia. Electronic address: subach_om@nrcki.ru.
FAU - Varfolomeeva, Larisa
AU  - Varfolomeeva L
AD  - Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian 
      Academy of Sciences, Moscow, 119071, Russia. Electronic address: 
      l.varfolomeeva@fbras.ru.
FAU - Vlaskina, Anna V
AU  - Vlaskina AV
AD  - Complex of NBICS Technologies, National Research Center "Kurchatov Institute", 
      Moscow, 123182, Russia. Electronic address: vlaskina_av@nrcki.ru.
FAU - Agapova, Yulia K
AU  - Agapova YK
AD  - Complex of NBICS Technologies, National Research Center "Kurchatov Institute", 
      Moscow, 123182, Russia. Electronic address: agapova.jk@gmail.com.
FAU - Nikolaeva, Alena Y
AU  - Nikolaeva AY
AD  - Complex of NBICS Technologies, National Research Center "Kurchatov Institute", 
      Moscow, 123182, Russia; Bach Institute of Biochemistry, Research Centre of 
      Biotechnology of the Russian Academy of Sciences, Moscow, 119071, Russia. 
      Electronic address: nikolaeva_ay@nrcki.ru.
FAU - Piatkevich, Kiryl D
AU  - Piatkevich KD
AD  - School of Life Sciences, Westlake University, Hangzhou, 310024, China; Westlake 
      Laboratory of Life Sciences and Biomedicine, Hangzhou, 310024, China; Institute 
      of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, 
      310024, China. Electronic address: kiryl.piatkevich@westlake.edu.cn.
FAU - Patrushev, Maxim V
AU  - Patrushev MV
AD  - Complex of NBICS Technologies, National Research Center "Kurchatov Institute", 
      Moscow, 123182, Russia. Electronic address: patrushev_mv@nrcki.ru.
FAU - Boyko, Konstantin M
AU  - Boyko KM
AD  - Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian 
      Academy of Sciences, Moscow, 119071, Russia. Electronic address: kmb@inbi.ras.ru.
FAU - Subach, Fedor V
AU  - Subach FV
AD  - Complex of NBICS Technologies, National Research Center "Kurchatov Institute", 
      Moscow, 123182, Russia. Electronic address: subach_fv@nrcki.ru.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230428
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - SY7Q814VUP (Calcium)
RN  - 0 (Calmodulin)
SB  - IM
MH  - Animals
MH  - Green Fluorescent Proteins/metabolism
MH  - *Calcium/metabolism
MH  - *Calmodulin/metabolism
MH  - Neurons/metabolism
MH  - Calcium Signaling
MH  - Mammals/metabolism
OTO - NOTNLM
OT  - Calcium imaging
OT  - Chromophore
OT  - Crystal structure
OT  - Genetically encoded calcium indicators
OT  - Green fluorescent ratiometric FNCaMP indicator
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/05/11 00:41
MHDA- 2023/05/29 06:42
CRDT- 2023/05/10 18:06
PHST- 2023/04/18 00:00 [received]
PHST- 2023/04/28 00:00 [revised]
PHST- 2023/04/28 00:00 [accepted]
PHST- 2023/05/29 06:42 [medline]
PHST- 2023/05/11 00:41 [pubmed]
PHST- 2023/05/10 18:06 [entrez]
AID - S0006-291X(23)00545-4 [pii]
AID - 10.1016/j.bbrc.2023.04.108 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2023 Jul 12;665:169-177. doi: 
      10.1016/j.bbrc.2023.04.108. Epub 2023 Apr 28.