PMID- 37165750
OWN - NLM
STAT- MEDLINE
DCOM- 20230714
LR  - 20230718
IS  - 2162-3279 (Electronic)
VI  - 13
IP  - 7
DP  - 2023 Jul
TI  - Combined treatment of high-intensity interval training with neural stem cell 
      generation on contusive model of spinal cord injury in rats.
PG  - e3043
LID - 10.1002/brb3.3043 [doi]
LID - e3043
AB  - INTRODUCTION: Spinal cord injury (SCI) leads to inflammation, axonal 
      degeneration, and gliosis. A combined treatment of exercise and neural stem cells 
      (NSC) has been proposed to improve neural repair. This study evaluated a combined 
      treatment of high-intensity interval training (HIIT) with NSC generation from 
      adipose-derived stem cells (ADSCs) on a contusive model of SCI in rats. MATERIALS 
      AND METHODS: In vitro, rat ADSCs were isolated from the perinephric regions of 
      Sprague-Dawley rats using enzymatic digestion. The ADSCs were transdifferentiated 
      into neurospheres using B27, EGF, and bFGF. After production of NSC, they were 
      labeled using green fluorescent protein (GFP). For the in vivo study, rats were 
      divided into eight groups: control group, sham operation group, sham operation + 
      HIIT group, sham operation + NSC group, SCI group, SCI + HIIT group, SCI + NSC 
      group, and SCI/HIIT/NSC group. Laminectomy was carried out at the T12 level using 
      the impactor system. HIIT was performed three times per week. To assess 
      behavioral function, the Basso-Beattie-Bresnahan (BBB) locomotor test and 
      H-reflex was carried out once a week for 12 weeks. We examined glial fibrillary 
      acidic protein (GFAP), S100beta, and NF200 expression. RESULTS: NSC transplantation, 
      HIIT and combined therapy with NSC transplantation, and the HIIT protocol 
      improved locomotor function with decreased maximum H to maximum M reflexes (H/M 
      ratio) and increased the Basso-Beattie-Bresnahan score. CONCLUSION: Combined 
      therapy in contused rats using the HIIT protocol and neurosphere-derived NSC 
      transplantation improves functional and histological outcomes.
CI  - (c) 2023 The Authors. Brain and Behavior published by Wiley Periodicals LLC.
FAU - Keikhaei, Reza
AU  - Keikhaei R
AD  - School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
FAU - Abdi, Elahe
AU  - Abdi E
AD  - Isfahan Neurosciences Research Center, Isfahan University of Medical Sciences, 
      Isfahan, Iran.
FAU - Darvishi, Marzieh
AU  - Darvishi M
AUID- ORCID: 0000-0001-7656-0854
AD  - Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.
AD  - Department of Anatomy, Faculty of Medicine, Ilam University of Medical Sciences, 
      Ilam, Iran.
FAU - Ghotbeddin, Zohreh
AU  - Ghotbeddin Z
AUID- ORCID: 0000-0003-2110-6333
AD  - Department of Physiology, Faculty of Veterinary Medicine, Shahid Chamran 
      University of Ahvaz, Ahvaz, Iran.
AD  - Stem Cell and Transgenic Technology Research Center, Shahid Chamran University of 
      Ahvaz, Ahvaz, Iran.
FAU - Hamidabadi, Hatef Ghasemi
AU  - Hamidabadi HG
AD  - Department of Anatomy & Cell Biology, Faculty of Medicine, Mazandaran University 
      of Medical Sciences, Sari, Iran.
AD  - Immunogenetic Research Center, Department of Anatomy & Cell Biology, Faculty of 
      Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230511
PL  - United States
TA  - Brain Behav
JT  - Brain and behavior
JID - 101570837
SB  - IM
MH  - Rats
MH  - Animals
MH  - Rats, Sprague-Dawley
MH  - *High-Intensity Interval Training
MH  - *Spinal Cord Injuries/therapy
MH  - *Neural Stem Cells/transplantation
MH  - Stem Cell Transplantation/methods
MH  - Spinal Cord
MH  - Recovery of Function
PMC - PMC10338768
OTO - NOTNLM
OT  - High-intensity interval training (HIIT)
OT  - neural stem cells (NSCS)
OT  - neurospheres
COIS- Authors declare that they have no conflict of interest.
EDAT- 2023/05/11 06:42
MHDA- 2023/07/14 13:08
PMCR- 2023/05/11
CRDT- 2023/05/11 03:58
PHST- 2023/04/17 00:00 [revised]
PHST- 2022/07/30 00:00 [received]
PHST- 2023/04/20 00:00 [accepted]
PHST- 2023/07/14 13:08 [medline]
PHST- 2023/05/11 06:42 [pubmed]
PHST- 2023/05/11 03:58 [entrez]
PHST- 2023/05/11 00:00 [pmc-release]
AID - BRB33043 [pii]
AID - 10.1002/brb3.3043 [doi]
PST - ppublish
SO  - Brain Behav. 2023 Jul;13(7):e3043. doi: 10.1002/brb3.3043. Epub 2023 May 11.