PMID- 37166086 OWN - NLM STAT- MEDLINE DCOM- 20230707 LR - 20240512 IS - 1943-4936 (Electronic) IS - 1040-6387 (Print) IS - 1040-6387 (Linking) VI - 35 IP - 4 DP - 2023 Jul TI - Effect of extrinsic factors on the detection of PRRSV and a porcine-specific internal sample control in serum, oral fluid, and fecal specimens tested by RT-rtPCR. PG - 374-383 LID - 10.1177/10406387231174556 [doi] AB - We characterized the effect of 1) temperature x time, 2) freeze-thaw cycles, and 3) high porcine reproductive and respiratory syndrome virus (PRRSV) RNA concentrations on the detection of PRRSV and a porcine-specific internal sample control (ISC) in serum, oral fluid, and fecal samples using a commercial PRRSV RT-rtPCR assay (Idexx). In study 1, the effect of temperature x time on PRRSV and ISC detection was shown to be specimen dependent. In serum stored at 4, 10, or 20 degrees C, PRRSV detection was consistent for up to 168 h, but storage at 30 degrees C reduced detectable PRRSV RNA. ISC RNA was stable in serum held at 4 and 10 degrees C, but not at 20 and 30 degrees C. In contrast, PRRSV and ISC RNAs in oral fluid and fecal samples continuously decreased at all temperature x time treatments. Based on these data, serum samples should be stored at