PMID- 3716793
OWN - NLM
STAT- MEDLINE
DCOM- 19860627
LR  - 20190903
IS  - 0001-6632 (Print)
IS  - 0001-6632 (Linking)
VI  - 36
IP  - 3
DP  - 1986 Mar
TI  - Effects of actinomycin D on localization of acridine orange chromatin interaction 
      complex in rat astrocytoma C6 cells.
PG  - 389-98
AB  - The purpose of this study was to examine the effects of actinomycin D on 
      localization of acridine orange (AO) binding to DNA in rat astrocytoma C6 cells 
      and to discuss briefly the significance of AO chromatin interaction products. 
      Actinomycin D markedly inhibited 3H-uridine incorporation into RNA and the 
      percentage of AO positive cells was reduced to approximately 40% of that of the 
      untreated control cells, whereas no distinct decrease of 3H-thymidine 
      incorporation was induced by actinomycin D. Electron microscopic radioautography 
      combined with the AO ultracytochemistry revealed that silver grains indicating 
      binding of 3H-actinomycin D are located mostly over the euchromatin portion near 
      the segregated nucleolus and heterochromatin and that no or only a few AO 
      chromatin complex was found in nuclei labeled heavily with 3H-actinomycin D. 
      These results of the present study together with the results of the previous 
      studies seem to indicate that AO might selectively bind to active or derepressed 
      DNA template sites for DNA dependent RNA polymerase in the euchromatin portion of 
      the cell nucleus.
FAU - Moriki, T
AU  - Moriki T
FAU - Hiroi, M
AU  - Hiroi M
FAU - Yamane, T
AU  - Yamane T
FAU - Hara, H
AU  - Hara H
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - Acta Pathol Jpn
JT  - Acta pathologica japonica
JID - 0372637
RN  - 0 (Chromatin)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Euchromatin)
RN  - 1CC1JFE158 (Dactinomycin)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*metabolism
MH  - Animals
MH  - Astrocytoma/*metabolism/ultrastructure
MH  - Autoradiography
MH  - Binding Sites
MH  - Cells, Cultured
MH  - Chromatin/*drug effects/metabolism
MH  - DNA, Neoplasm/metabolism
MH  - Dactinomycin/*pharmacology
MH  - Euchromatin
MH  - Histocytochemistry
MH  - Microscopy, Electron
MH  - Rats
EDAT- 1986/03/01 00:00
MHDA- 1986/03/01 00:01
CRDT- 1986/03/01 00:00
PHST- 1986/03/01 00:00 [pubmed]
PHST- 1986/03/01 00:01 [medline]
PHST- 1986/03/01 00:00 [entrez]
AID - 10.1111/j.1440-1827.1986.tb01028.x [doi]
PST - ppublish
SO  - Acta Pathol Jpn. 1986 Mar;36(3):389-98. doi: 10.1111/j.1440-1827.1986.tb01028.x.