PMID- 37171872
OWN - NLM
STAT- MEDLINE
DCOM- 20230904
LR  - 20230904
IS  - 1944-7922 (Electronic)
IS  - 1060-3271 (Linking)
VI  - 106
IP  - 5
DP  - 2023 Sep 1
TI  - Validation of the AnticFast(R) Beta-Lactams Rapid Test Kit for Detection of 
      Beta-Lactams (Penicillins and Cephalosporins) in Raw Cow's Milk: AOAC Performance 
      Tested MethodSM 032303.
PG  - 1197-1208
LID - 10.1093/jaoacint/qsad054 [doi]
AB  - BACKGROUND: AnticFast(R) Beta-Lactams Rapid Test Kit is a qualitative two-step 
      (2 min + 5 min) rapid lateral flow assay to detect beta-lactam (penicillins and 
      cephalosporins) antibiotic residues in raw commingled cow's milk. OBJECTIVE: The 
      method performance was evaluated according to Commission Decision 2002/657/EC, 
      Commission Implementing Regulation 2021/808, and Community Reference Laboratories 
      Residues Guidelines for the Validation of Screening Methods for Residues of 
      Veterinary Medicines. METHODS: The AnticFast Beta-Lactams Rapid Test Kit was 
      evaluated for detection capability, selectivity, false-positive results, 
      repeatability, robustness, suitability for various milk types and milk 
      compositions, milks from various species, and test kit consistency and stability. 
      Samples included milks spiked at concentrations bracketing the EU maximum residue 
      limits (MRLs) for beta-lactams as well as bulk farm and tanker milks. RESULTS: The 
      AnticFast Beta-Lactams Rapid Test Kit is specific for the detection of beta-lactams 
      in milk and does not detect compounds from other antibiotic families. 
      Interference was seen with clavulanic acid, a beta-lactamase inhibitor, which was 
      expected. The test can detect all residues of beta-lactams (penicillins and 
      cephalosporins) present on the EU-MRL list for milk at their respective MRL 
      except for desfuroylceftiofur and cephalexin, which were above the MRL. No false 
      positives were detected in the 602 (300 blank farm and 302 tanker load) samples 
      tested. Robustness testing indicated that the detection in heat-treated milk 
      types may be slightly hampered. For substances with a detection capability well 
      below the MRL, this interference does not cause problems since detection at MRL 
      remains guaranteed, but care should be taken for substances with a CCbeta at or near 
      their MRL. Diminished sample flow was seen with reconstituted milk powder and 
      blank ewes' milk, so sample flow should always be verified for these milk types. 
      CONCLUSIONS: Results of this validation show that the AnticFast Beta-Lactams 
      Rapid Test Kit is a reliable test for rapid screening of raw cows' milk for 
      residues of beta-lactam antibiotics. HIGHLIGHTS: AnticFast Beta-Lactams Rapid Test 
      Kit is an easy, realiable, robust and highly specific test for screening of raw 
      cows' milk for residues of penicillins and cephalosporins.
CI  - (c) The Author(s) 2023. Published by Oxford University Press on behalf of AOAC 
      INTERNATIONAL. All rights reserved. For permissions, please email: 
      journals.permissions@oup.com.
FAU - Broekaert, Katrien
AU  - Broekaert K
AUID- ORCID: 0000-0002-9881-2327
AD  - Research Institute for Agriculture, Fisheries and Food (ILVO), Technology and 
      Food Science Unit, Brusselsesteenweg 370, 9090 Melle, Belgium.
FAU - Ooghe, Sigrid
AU  - Ooghe S
AUID- ORCID: 0000-0002-4986-9677
AD  - Research Institute for Agriculture, Fisheries and Food (ILVO), Technology and 
      Food Science Unit, Brusselsesteenweg 370, 9090 Melle, Belgium.
FAU - Yang, Meng
AU  - Yang M
AUID- ORCID: 0000-0001-5959-4024
AD  - Beijing Meizheng Bio-Tech, Ltd, No. 2 Building, No. 8 Courtyard, Fenggusilu Road, 
      Yanqing District, Beijing 102101, P.R. China.
FAU - Tian, Xiumei
AU  - Tian X
AUID- ORCID: 0000-0002-1959-4967
AD  - Beijing Meizheng Bio-Tech, Ltd, No. 2 Building, No. 8 Courtyard, Fenggusilu Road, 
      Yanqing District, Beijing 102101, P.R. China.
FAU - Reybroeck, Wim
AU  - Reybroeck W
AUID- ORCID: 0000-0002-2269-6534
AD  - Research Institute for Agriculture, Fisheries and Food (ILVO), Technology and 
      Food Science Unit, Brusselsesteenweg 370, 9090 Melle, Belgium.
LA  - eng
PT  - Journal Article
PL  - England
TA  - J AOAC Int
JT  - Journal of AOAC International
JID - 9215446
RN  - 0 (beta-Lactams)
RN  - 0 (Penicillins)
RN  - 0 (Cephalosporins)
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Monobactams)
SB  - IM
MH  - Animals
MH  - Female
MH  - Sheep
MH  - Cattle
MH  - *beta-Lactams/analysis
MH  - Penicillins/analysis
MH  - Cephalosporins/analysis
MH  - Milk/chemistry
MH  - *Drug Residues/analysis
MH  - Anti-Bacterial Agents/analysis
MH  - Monobactams/analysis
MH  - Food Contamination/analysis
EDAT- 2023/05/12 13:08
MHDA- 2023/09/04 06:42
CRDT- 2023/05/12 11:55
PHST- 2023/04/03 00:00 [received]
PHST- 2023/04/04 00:00 [accepted]
PHST- 2023/09/04 06:42 [medline]
PHST- 2023/05/12 13:08 [pubmed]
PHST- 2023/05/12 11:55 [entrez]
AID - 7160899 [pii]
AID - 10.1093/jaoacint/qsad054 [doi]
PST - ppublish
SO  - J AOAC Int. 2023 Sep 1;106(5):1197-1208. doi: 10.1093/jaoacint/qsad054.