PMID- 37174617
OWN - NLM
STAT- MEDLINE
DCOM- 20230515
LR  - 20230516
IS  - 2073-4409 (Electronic)
IS  - 2073-4409 (Linking)
VI  - 12
IP  - 9
DP  - 2023 Apr 22
TI  - Development of an In Vitro Blood Vessel Model Using Autologous Endothelial Cells 
      Generated from Footprint-Free hiPSCs to Analyze Interactions of the Endothelium 
      with Blood Cell Components and Vascular Implants.
LID - 10.3390/cells12091217 [doi]
LID - 1217
AB  - Cardiovascular diseases are the leading cause of death globally. Vascular 
      implants, such as stents, are required to treat arterial stenosis or dilatation. 
      The development of innovative stent materials and coatings, as well as novel 
      preclinical testing strategies, is needed to improve the bio- and 
      hemocompatibility of current stents. In this study, a blood vessel-like 
      polydimethylsiloxane (PDMS) model was established to analyze the interaction of 
      an endothelium with vascular implants, as well as blood-derived cells, in vitro. 
      Using footprint-free human induced pluripotent stem cells (hiPSCs) and subsequent 
      differentiation, functional endothelial cells (ECs) expressing specific markers 
      were generated and used to endothelialize an artificial PDMS lumen. The 
      established model was used to demonstrate the interaction of the created 
      endothelium with blood-derived immune cells, which also allowed for real-time 
      imaging. In addition, a stent was inserted into the endothelialized lumen to 
      analyze the surface endothelialization of stents. In the future, this blood 
      vessel-like model could serve as an in vitro platform to test the influence of 
      vascular implants and coatings on endothelialization and to analyze the 
      interaction of the endothelium with blood cell components.
FAU - Weber, Josefin
AU  - Weber J
AD  - Department of Thoracic and Cardiovascular Surgery, University Hospital Tuebingen, 
      Calwerstrasse 7/1, 72076 Tuebingen, Germany.
FAU - Weber, Marbod
AU  - Weber M
AUID- ORCID: 0000-0002-5450-4517
AD  - Department of Thoracic and Cardiovascular Surgery, University Hospital Tuebingen, 
      Calwerstrasse 7/1, 72076 Tuebingen, Germany.
FAU - Feile, Adrian
AU  - Feile A
AD  - Department of Thoracic and Cardiovascular Surgery, University Hospital Tuebingen, 
      Calwerstrasse 7/1, 72076 Tuebingen, Germany.
FAU - Schlensak, Christian
AU  - Schlensak C
AD  - Department of Thoracic and Cardiovascular Surgery, University Hospital Tuebingen, 
      Calwerstrasse 7/1, 72076 Tuebingen, Germany.
FAU - Avci-Adali, Meltem
AU  - Avci-Adali M
AUID- ORCID: 0000-0003-4008-4272
AD  - Department of Thoracic and Cardiovascular Surgery, University Hospital Tuebingen, 
      Calwerstrasse 7/1, 72076 Tuebingen, Germany.
LA  - eng
PT  - Journal Article
DEP - 20230422
PL  - Switzerland
TA  - Cells
JT  - Cells
JID - 101600052
SB  - IM
MH  - Humans
MH  - *Endothelial Cells/metabolism
MH  - *Induced Pluripotent Stem Cells/metabolism
MH  - Endothelium
MH  - Stents
MH  - Cell Differentiation
PMC - PMC10177426
OTO - NOTNLM
OT  - endothelial cells (ECs)
OT  - human induced pluripotent stem cells (hiPSCs)
OT  - in vitro model
OT  - regenerative medicine
OT  - tissue engineering
OT  - vascular implants
COIS- The authors declare no conflict of interest.
EDAT- 2023/05/13 15:13
MHDA- 2023/05/15 11:42
PMCR- 2023/04/22
CRDT- 2023/05/13 01:22
PHST- 2023/02/20 00:00 [received]
PHST- 2023/04/18 00:00 [revised]
PHST- 2023/04/20 00:00 [accepted]
PHST- 2023/05/15 11:42 [medline]
PHST- 2023/05/13 15:13 [pubmed]
PHST- 2023/05/13 01:22 [entrez]
PHST- 2023/04/22 00:00 [pmc-release]
AID - cells12091217 [pii]
AID - cells-12-01217 [pii]
AID - 10.3390/cells12091217 [doi]
PST - epublish
SO  - Cells. 2023 Apr 22;12(9):1217. doi: 10.3390/cells12091217.