PMID- 37175425 OWN - NLM STAT- MEDLINE DCOM- 20230515 LR - 20230515 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 24 IP - 9 DP - 2023 Apr 23 TI - The Anti-Atopic Dermatitis Effects of Mentha arvensis Essential Oil Are Involved in the Inhibition of the NLRP3 Inflammasome in DNCB-Challenged Atopic Dermatitis BALB/c Mice. LID - 10.3390/ijms24097720 [doi] LID - 7720 AB - The NLRP3 inflammasome is upregulated by various agents, such as nuclear factor-kappa B (NF-kappaB), lipopolysaccharide (LPS), and adenosine triphosphate (ATP). The NLRP3 inflammasome facilitations the maturation of interleukin (IL)-1beta, a proinflammatory cytokine that is critically involved in the pathogenesis of atopic dermatitis (AD). Although the NLRP3 inflammasome clearly exacerbates AD symptoms such as erythema and pruritus, drugs for AD patients targeting the NLRP3 inflammasome are still lacking. Based on the previous findings that Mentha arvensis essential oil (MAEO) possesses strong anti-inflammatory and anti-AD properties through its inhibition of the ERK/NF-kappaB signaling pathway, we postulated that MAEO might be capable of modulating the NLRP3 inflammasome in AD. The aim of this research was to investigate whether MAEO affects the inhibition of NLRP3 inflammasome activation in murine bone marrow-derived macrophages (BMDMs) stimulated with LPS + ATP in vitro and in a murine model displaying AD-like symptoms induced by 2,4-dinitrochlorobenzene (DNCB) in vivo. We found that MAEO inhibited the expression of NLRP3 and caspase-1, leading to the suppression of NLRP3 inflammasome activation and IL-1beta production in BMDMs stimulated with LPS + ATP. In addition, MAEO exhibited efficacy in ameliorating AD symptoms in a murine model induced by DNCB, as indicated by the reduction in dermatitis score, ear thickness, transepidermal water loss (TEWL), epidermal thickness, and immunoglobulin E (IgE) levels. Furthermore, MAEO attenuated the recruitment of NLRP3-expressing macrophages and NLRP3 inflammasome activation in murine dorsal skin lesions induced by DNCB. Overall, we provide evidence for the anti-AD effects of MAEO via inhibition of NLRP3 inflammasome activation. FAU - Kim, So-Yeon AU - Kim SY AUID- ORCID: 0000-0003-1885-0540 AD - Department of Food Biotechnology and Environmental Science, Kangwon National University, Chuncheon 24341, Republic of Korea. FAU - Sapkota, Arjun AU - Sapkota A AD - College of Pharmacy and Gachon Institute of Pharmaceutical Sciences, Gachon University, Incheon 21936, Republic of Korea. FAU - Bae, Young Joo AU - Bae YJ AD - College of Pharmacy and Gachon Institute of Pharmaceutical Sciences, Gachon University, Incheon 21936, Republic of Korea. FAU - Choi, Seung-Hyuk AU - Choi SH AD - Department of Food Biotechnology and Environmental Science, Kangwon National University, Chuncheon 24341, Republic of Korea. FAU - Bae, Ho Jung AU - Bae HJ AD - Agriculture and Life Science Research Institute, Kangwon National University, Chuncheon 24341, Republic of Korea. FAU - Kim, Hyun-Jeong AU - Kim HJ AD - Department of Food Biotechnology and Environmental Science, Kangwon National University, Chuncheon 24341, Republic of Korea. FAU - Cho, Ye Eun AU - Cho YE AD - Department of Food Biotechnology and Environmental Science, Kangwon National University, Chuncheon 24341, Republic of Korea. FAU - Choi, Yu-Yeong AU - Choi YY AD - Department of Food Biotechnology and Environmental Science, Kangwon National University, Chuncheon 24341, Republic of Korea. FAU - An, Ju-Yeon AU - An JY AD - Department of Food Biotechnology and Environmental Science, Kangwon National University, Chuncheon 24341, Republic of Korea. FAU - Cho, So-Young AU - Cho SY AD - Department of Food Biotechnology and Environmental Science, Kangwon National University, Chuncheon 24341, Republic of Korea. FAU - Hong, Sun Hee AU - Hong SH AUID- ORCID: 0000-0001-7581-0604 AD - School of Applied Science in Natural Resources & Environment, Hankyong National University, Anseong 17579, Republic of Korea. FAU - Choi, Ji Woong AU - Choi JW AD - College of Pharmacy and Gachon Institute of Pharmaceutical Sciences, Gachon University, Incheon 21936, Republic of Korea. FAU - Park, Se Jin AU - Park SJ AUID- ORCID: 0000-0002-9370-5258 AD - Department of Food Biotechnology and Environmental Science, Kangwon National University, Chuncheon 24341, Republic of Korea. AD - Agriculture and Life Science Research Institute, Kangwon National University, Chuncheon 24341, Republic of Korea. AD - School of Natural Resources and Environmental Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea. LA - eng GR - 2018002270002/Korean Ministry of Environment/ GR - NRF-2021R1A6A1A03044242/Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education/ PT - Journal Article DEP - 20230423 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Inflammasomes) RN - 0 (Dinitrochlorobenzene) RN - 0 (NF-kappa B) RN - 0 (NLR Family, Pyrin Domain-Containing 3 Protein) RN - 0 (Lipopolysaccharides) RN - 0 (Cytokines) RN - 0 (Nlrp3 protein, mouse) SB - IM MH - Animals MH - Mice MH - *Inflammasomes/metabolism MH - Dinitrochlorobenzene/adverse effects MH - NF-kappa B/metabolism MH - NLR Family, Pyrin Domain-Containing 3 Protein/metabolism MH - Mice, Inbred BALB C MH - Lipopolysaccharides/toxicity MH - Disease Models, Animal MH - *Dermatitis, Atopic/chemically induced/drug therapy/metabolism MH - Cytokines/metabolism PMC - PMC10177797 OTO - NOTNLM OT - BMDMs OT - IL-1beta OT - Mentha arvensis OT - NLRP3 inflammasome OT - atopic dermatitis COIS- The authors declare no conflict of interest. EDAT- 2023/05/13 15:12 MHDA- 2023/05/15 11:42 PMCR- 2023/04/23 CRDT- 2023/05/13 01:26 PHST- 2023/03/22 00:00 [received] PHST- 2023/04/18 00:00 [revised] PHST- 2023/04/19 00:00 [accepted] PHST- 2023/05/15 11:42 [medline] PHST- 2023/05/13 15:12 [pubmed] PHST- 2023/05/13 01:26 [entrez] PHST- 2023/04/23 00:00 [pmc-release] AID - ijms24097720 [pii] AID - ijms-24-07720 [pii] AID - 10.3390/ijms24097720 [doi] PST - epublish SO - Int J Mol Sci. 2023 Apr 23;24(9):7720. doi: 10.3390/ijms24097720.