PMID- 37176240 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20230515 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 16 IP - 9 DP - 2023 Apr 25 TI - Inhibited and Retarded Behavior by Ca(2+) and Ca(2+)/OD Loading Rate on Ureolytic Bacteria in MICP Process. LID - 10.3390/ma16093357 [doi] LID - 3357 AB - The estimation of optical density (OD) with viable cells is challenging for engineering purposes. In this study, the OD conversion based on previous study was used. The inhibited and retarded behavior of the microbially induced carbonate precipitation (MICP) process was examined. The experimental results showed that high Ca(2+) drastically influences the inhibited and retarded behavior on MICP processes. The analysis showed that the inhibition and retardation effects occurred when the Ca(2+)/OD loading rate exceeded 8.46 M. The critical value was equal to the proportional constant for obtaining carbonate precipitation rate (CPR) from OD. Due to this, the blending design of materials became possible, with no risk of inhibition. In conclusion, the inhibition and retardation of the MICP process are governed by the Ca(2+) load and the linear standard line (LSL), which may be attributed to the capacity or tolerance of viable cells, i.e., CPR/OD = 8.46 M or Ca(2+)/OD = 8.46 M. FAU - Fukue, Masaharu AU - Fukue M AD - Japanese Geotechnical Association for Housing Disaster Prevention, 1622, Oshikiri, Shimizu-ku, Shizuoka 424-0008, Shizuoka, Japan. FAU - Lechowicz, Zbigniew AU - Lechowicz Z AUID- ORCID: 0000-0002-1426-5881 AD - Department of Geotechnical Engineering, Institute of Civil Engineering, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland. FAU - Fujimori, Yuichi AU - Fujimori Y AD - Chubu Sokuchi Research Institute Co., 801-1 Konami, Suwa City 392-0131, Nagano, Japan. FAU - Emori, Kentaro AU - Emori K AD - Sanko Kaihatsu Co., Ltd., 1320 Gokanjima, Fuji City 416-0946, Shizuoka, Japan. FAU - Mulligan, Catherine N AU - Mulligan CN AUID- ORCID: 0000-0001-5337-4776 AD - Department of Building, Civil and Environment Engineering, Concordia University, 1455 de Maisonneuve Blvd. W., Montreal, QC H3G 1M8, Canada. LA - eng PT - Journal Article DEP - 20230425 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC10180355 OTO - NOTNLM OT - Ca2+ loading OT - MICP OT - OD-conversion OT - inhibition OT - retarded reaction OT - viability of cells COIS- The authors declare no conflict of interest. EDAT- 2023/05/13 15:12 MHDA- 2023/05/13 15:13 PMCR- 2023/04/25 CRDT- 2023/05/13 01:32 PHST- 2023/03/08 00:00 [received] PHST- 2023/04/11 00:00 [revised] PHST- 2023/04/22 00:00 [accepted] PHST- 2023/05/13 15:13 [medline] PHST- 2023/05/13 15:12 [pubmed] PHST- 2023/05/13 01:32 [entrez] PHST- 2023/04/25 00:00 [pmc-release] AID - ma16093357 [pii] AID - materials-16-03357 [pii] AID - 10.3390/ma16093357 [doi] PST - epublish SO - Materials (Basel). 2023 Apr 25;16(9):3357. doi: 10.3390/ma16093357.