PMID- 37176933
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230515
IS  - 2223-7747 (Print)
IS  - 2223-7747 (Electronic)
IS  - 2223-7747 (Linking)
VI  - 12
IP  - 9
DP  - 2023 May 4
TI  - Identification, Characterization, and Cytological Analysis of Several Unexpected 
      Hybrids Derived from Reciprocal Crosses between Raphanobrassica and Its Diploid 
      Parents.
LID - 10.3390/plants12091875 [doi]
LID - 1875
AB  - Interspecific hybridization and accompanying backcross between crops and 
      relatives have been recognized as a powerful method to broaden genetic diversity 
      and transfer desirable adaptive traits. Crosses between radish (Raphanus sativus, 
      RR, 2n = 18) and Brassica oleracea (CC, 2n = 18), which formed allotetraploid 
      Raphanobrassica (RRCC, 2n = 36), initiated the construction of resynthetic 
      allopolyploids. However, these progenies from the backcrosses between 
      Raphanobrassica and the two diploid parents have not been well deciphered. 
      Herein, thousands of backcrosses using both Raphanobrassica and the two diploid 
      parents as pollen donors were employed. Several hybrids with expected (2n = 27) 
      and unexpected chromosome numbers (2n = 26 and 2n = 36) were obtained. 
      Fluorescence in situ hybridization (FISH) analysis with R-genome-specific 
      sequences as probes demonstrated that the genome structures of the two expected 
      hybrids were RRC and CCR, and the genome structures of the three unexpected 
      hybrids were RRRC, CCCR, and RRC' (harbouring an incomplete C genome). The 
      unexpected hybrids with extra R or C genomes showed similar phenotypic 
      characteristics to their expected hybrids. FISH analysis with C-genome-specific 
      sequences as probes demonstrated that the unexpected allotetraploid hybrids 
      exhibited significantly more intergenomic chromosome pairings than the expected 
      hybrids. The expected and unexpected hybrids provide not only novel germplasm 
      resources for the breeding of radish and B. oleracea but also very important 
      genetic material for genome dosage analysis.
FAU - Yu, Jie
AU  - Yu J
AUID- ORCID: 0000-0002-0463-9704
AD  - School of Life Sciences, Guizhou Normal University, Guiyang 550025, China.
FAU - Lei, Shaolin
AU  - Lei S
AD  - Guizhou Institute of Oil Crops, Guizhou Academy of Agricultural Sciences, Guiyang 
      550009, China.
FAU - Fang, Shiting
AU  - Fang S
AD  - School of Life Sciences, Guizhou Normal University, Guiyang 550025, China.
FAU - Tai, Niufang
AU  - Tai N
AD  - School of Life Sciences, Guizhou Normal University, Guiyang 550025, China.
FAU - Yu, Wei
AU  - Yu W
AD  - School of Life Sciences, Guizhou Normal University, Guiyang 550025, China.
FAU - Yang, Ziwei
AU  - Yang Z
AD  - School of Life Sciences, Guizhou Normal University, Guiyang 550025, China.
FAU - Gu, Lei
AU  - Gu L
AD  - School of Life Sciences, Guizhou Normal University, Guiyang 550025, China.
FAU - Wang, Hongcheng
AU  - Wang H
AD  - School of Life Sciences, Guizhou Normal University, Guiyang 550025, China.
FAU - Du, Xuye
AU  - Du X
AUID- ORCID: 0000-0003-4328-0236
AD  - School of Life Sciences, Guizhou Normal University, Guiyang 550025, China.
FAU - Zhu, Bin
AU  - Zhu B
AD  - School of Life Sciences, Guizhou Normal University, Guiyang 550025, China.
FAU - Cai, Mengxian
AU  - Cai M
AD  - School of Life Sciences, Guizhou Normal University, Guiyang 550025, China.
LA  - eng
GR  - ZK[2023]YB272/Guizhou Provincial Basic Research Program (Natural Science)/
GR  - [2022]ZD031/Guizhou Provincial Key Technology R&D Program/
GR  - 001/the earmarked fund for GZMARS-Rapeseed/
PT  - Journal Article
DEP - 20230504
PL  - Switzerland
TA  - Plants (Basel)
JT  - Plants (Basel, Switzerland)
JID - 101596181
PMC - PMC10181067
OTO - NOTNLM
OT  - Brassica oleracea
OT  - chromosome behaviour
OT  - interspecific hybridization
OT  - radish
OT  - unexpected hybrids
COIS- The authors declare no conflict of interest.
EDAT- 2023/05/13 15:13
MHDA- 2023/05/13 15:14
PMCR- 2023/05/04
CRDT- 2023/05/13 01:35
PHST- 2023/04/08 00:00 [received]
PHST- 2023/04/28 00:00 [revised]
PHST- 2023/05/01 00:00 [accepted]
PHST- 2023/05/13 15:14 [medline]
PHST- 2023/05/13 15:13 [pubmed]
PHST- 2023/05/13 01:35 [entrez]
PHST- 2023/05/04 00:00 [pmc-release]
AID - plants12091875 [pii]
AID - plants-12-01875 [pii]
AID - 10.3390/plants12091875 [doi]
PST - epublish
SO  - Plants (Basel). 2023 May 4;12(9):1875. doi: 10.3390/plants12091875.