PMID- 37178216
OWN - NLM
STAT- MEDLINE
DCOM- 20230717
LR  - 20230718
IS  - 1432-203X (Electronic)
IS  - 0721-7714 (Linking)
VI  - 42
IP  - 8
DP  - 2023 Aug
TI  - The SMC5/6 complex subunit MMS21 regulates stem cell proliferation in rice.
PG  - 1279-1290
LID - 10.1007/s00299-023-03030-9 [doi]
AB  - SMC5/6 complex subunit OsMMS21 is involved in cell cycle and hormone signaling 
      and required for stem cell proliferation during shoot and root development in 
      rice. The structural maintenance of chromosome (SMC)5/6 complex is required for 
      nucleolar integrity and DNA metabolism. Moreover, METHYL METHANESULFONATE 
      SENSITIVITY GENE 21 (MMS21), a SUMO E3 ligase that is part of the SMC5/6 complex, 
      is essential for the root stem cell niche and cell cycle transition in 
      Arabidopsis. However, its specific role in rice remains unclear. Here, OsSMC5 and 
      OsSMC6 single heterozygous mutants were generated using CRISPR/Cas9 technology to 
      elucidate the function of SMC5/6 subunits, including OsSMC5, OsSMC6, and OsMMS21, 
      in cell proliferation in rice. ossmc5/ + and ossmc6/ + heterozygous single 
      mutants did not yield homozygous mutants in their progeny, indicating that OsSMC5 
      and OsSMC6 both play necessary roles during embryo formation. Loss of OsMMS21 
      caused severe defects in both the shoot and roots in rice. Transcriptome analysis 
      showed a significant decrease in the expression of genes involved in auxin 
      signaling in the roots of osmms21 mutants. Moreover, the expression levels of the 
      cycB2-1 and MCM genes, which are involved the cell cycle, were significantly 
      lower in the shoots of the mutants, indicating that OsMMS21 was involved in both 
      hormone signaling pathways and the cell cycle. Overall, these findings indicate 
      that the SUMO E3 ligase OsMMS21 is required for both shoot and root stem cell 
      niches, improving the understanding of the function of the SMC5/6 complex in 
      rice.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Xun, Qian
AU  - Xun Q
AD  - College of Agriculture, Nanjing Agricultural University, Nanjing, 210095, China.
FAU - Song, Ye
AU  - Song Y
AD  - College of Agriculture, Nanjing Agricultural University, Nanjing, 210095, China.
FAU - Mei, Min
AU  - Mei M
AD  - College of Agriculture, Nanjing Agricultural University, Nanjing, 210095, China.
FAU - Ding, Yanfeng
AU  - Ding Y
AD  - College of Agriculture, Nanjing Agricultural University, Nanjing, 210095, China.
AD  - Key Laboratory of Crop Physiology Ecology and Production Management, Ministry of 
      Agriculture, Nanjing, 210095, China.
AD  - Jiangsu Collaborative Innovation Centre for Modern Crop Production, Nanjing, 
      210095, China.
FAU - Ding, Chengqiang
AU  - Ding C
AUID- ORCID: 0000-0001-6061-1979
AD  - College of Agriculture, Nanjing Agricultural University, Nanjing, 210095, China. 
      dingcq@njau.edu.cn.
AD  - Key Laboratory of Crop Physiology Ecology and Production Management, Ministry of 
      Agriculture, Nanjing, 210095, China. dingcq@njau.edu.cn.
AD  - Jiangsu Collaborative Innovation Centre for Modern Crop Production, Nanjing, 
      210095, China. dingcq@njau.edu.cn.
LA  - eng
GR  - 31971842/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20230513
PL  - Germany
TA  - Plant Cell Rep
JT  - Plant cell reports
JID - 9880970
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (SUMO-1 Protein)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - 0 (Hormones)
SB  - IM
MH  - *Saccharomyces cerevisiae Proteins/genetics
MH  - *Oryza/genetics/metabolism
MH  - Cell Cycle Proteins/genetics/chemistry/metabolism
MH  - SUMO-1 Protein/chemistry/genetics/metabolism
MH  - Ubiquitin-Protein Ligases/genetics
MH  - Cell Division
MH  - Hormones
OTO - NOTNLM
OT  - OsMMS21
OT  - OsSMC5
OT  - OsSMC6
OT  - Rice
OT  - SMC5/6 complex
OT  - Shoot and root stem cells
EDAT- 2023/05/14 01:07
MHDA- 2023/07/17 06:42
CRDT- 2023/05/13 15:00
PHST- 2023/03/06 00:00 [received]
PHST- 2023/05/04 00:00 [accepted]
PHST- 2023/07/17 06:42 [medline]
PHST- 2023/05/14 01:07 [pubmed]
PHST- 2023/05/13 15:00 [entrez]
AID - 10.1007/s00299-023-03030-9 [pii]
AID - 10.1007/s00299-023-03030-9 [doi]
PST - ppublish
SO  - Plant Cell Rep. 2023 Aug;42(8):1279-1290. doi: 10.1007/s00299-023-03030-9. Epub 
      2023 May 13.