PMID- 37178795
OWN - NLM
STAT- MEDLINE
DCOM- 20230609
LR  - 20230717
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 332
DP  - 2023 Jul 15
TI  - Pathogenicity of two lineages of infectious hematopoietic necrosis virus (IHNV) 
      to farmed rainbow trout (Oncorhynchus mykiss) in South Korea.
PG  - 199133
LID - S0168-1702(23)00095-3 [pii]
LID - 10.1016/j.virusres.2023.199133 [doi]
LID - 199133
AB  - In May 2015, a high mortality event in farmed rainbow trout occurred in 
      Jeollabuk-do province in Korea. Histopathological analysis revealed necrosis in 
      the kidney, liver, branchial arch, and gills of moribund fish, and infectious 
      hematopoietic necrosis virus (IHNV) was detected in the lesions by 
      immunohistochemistry. Cytopathic effects were observed in EPC, FHM, and RTG-2 
      cell lines after inoculation with kidney and spleen tissues and IHNV was detected 
      by reverse transcription polymerase chain reaction (PCR). The amplified PCR 
      product was sequenced, and phylogenetic analysis placed IHNV in the JRt Nagano 
      group. Both in vivo and in vitro trials were performed to compare the virulence 
      properties between RtWanju15 isolate, which causes 100% mortality in imported 
      fry, and a previous isolate RtWanju09 of the JRt Shizuoka group isolated from 
      eggs of healthy broodfish. In vivo challenge with high dose on specific pathogen 
      free (SPF) rainbow trout fry performed in Denmark with isolates RtWanju09, 
      RtWanju15 and DF04/99 isolates showed a survival rates of 60%, 37.5% and 52.5% 
      (average), respectively without statistical difference. The replication 
      efficiency of the two isolates in the in vitro challenge was similar.
CI  - Copyright (c) 2023. Published by Elsevier B.V.
FAU - Kim, Hyoung Jun
AU  - Kim HJ
AD  - WOAH Reference Laboratory for VHS, National Institute of Fisheries Science, Busan 
      46083, Korea.
FAU - Olesen, Niels Jorgen
AU  - Olesen NJ
AD  - EU Reference Laboratory for VHS, Section for Fish and Shellfish Diseases, 
      National Institute for Aquatic Resources, Technical University of Denmark, 2800 
      Kgs, Lyngby, Denmark.
FAU - Dale, Ole Bendik
AU  - Dale OB
AD  - WOAH Reference Laboratory for ISA, Norwegian Veterinary Institute, Pb 64, N-1431 
      As. Norway.
FAU - Kim, Young Chul
AU  - Kim YC
AD  - Department of Aquatic Life Medicine, Gangneung-Wonju National University, 
      Gangneung 25457, Korea.
FAU - Jung, Tae Sung
AU  - Jung TS
AD  - Laboratory of Aquatic Animal Diseases, Research Institute of Natural Science, 
      Gyeongsang National University, Jinju 52828, Korea.
FAU - Vendramin, Niccolo
AU  - Vendramin N
AD  - EU Reference Laboratory for VHS, Section for Fish and Shellfish Diseases, 
      National Institute for Aquatic Resources, Technical University of Denmark, 2800 
      Kgs, Lyngby, Denmark.
FAU - Kwon, Se Ryun
AU  - Kwon SR
AD  - Department of Aquatic Life Medical Sciences, Sunmoon University, Asan-si, 
      Chungnam 31460, Korea. Electronic address: srkwon@sunmoon.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230518
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
SB  - IM
MH  - Animals
MH  - *Oncorhynchus mykiss
MH  - *Infectious hematopoietic necrosis virus/genetics
MH  - Virulence
MH  - *Rhabdoviridae Infections/veterinary
MH  - Phylogeny
MH  - *Fish Diseases
PMC - PMC10345748
OTO - NOTNLM
OT  - Immune-histopathological observation
OT  - Infectious hematopoietic necrosis virus (IHNV)
OT  - Mass mortality
OT  - Phylogenetic analysis
OT  - Rainbow trout
OT  - challenge test
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/05/14 01:07
MHDA- 2023/06/09 06:42
PMCR- 2023/05/18
CRDT- 2023/05/13 19:25
PHST- 2023/01/29 00:00 [received]
PHST- 2023/05/11 00:00 [revised]
PHST- 2023/05/11 00:00 [accepted]
PHST- 2023/06/09 06:42 [medline]
PHST- 2023/05/14 01:07 [pubmed]
PHST- 2023/05/13 19:25 [entrez]
PHST- 2023/05/18 00:00 [pmc-release]
AID - S0168-1702(23)00095-3 [pii]
AID - 199133 [pii]
AID - 10.1016/j.virusres.2023.199133 [doi]
PST - ppublish
SO  - Virus Res. 2023 Jul 15;332:199133. doi: 10.1016/j.virusres.2023.199133. Epub 2023 
      May 18.