PMID- 37179548
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230919
IS  - 1662-4548 (Print)
IS  - 1662-453X (Electronic)
IS  - 1662-453X (Linking)
VI  - 17
DP  - 2023
TI  - The contribution of probiotics for the double-edge effect of cefazolin on 
      postoperative neurocognitive disorders by rebalancing the gut microbiota.
PG  - 1156453
LID - 10.3389/fnins.2023.1156453 [doi]
LID - 1156453
AB  - INTRODUCTION: Emerging data suggest that perioperative gut dysbiosis is prevalent 
      and may be associated with postoperative neurocognitive disorders (PND). 
      Antibiotics and probiotics are key factors influencing the microbiota. Many 
      antibiotics have anti-microorganisms and direct anti-inflammatory properties, 
      which may have cognitive repercussions. NLRP3 inflammasome activation has been 
      reported to be involved with cognitive deficits. This study aimed to determine 
      the effect and mechanism of probiotics on neurocognitive problems associated with 
      perioperative gut dysbiosis by the NLRP3 pathway. METHODS: In a randomized, 
      controlled trial, adult male Kunming mice undergoing surgery were administered 
      cefazolin, FOS + probiotics, CY-09, or a placebo in four distinct experimental 
      cohorts. Fear conditioning (FC) tests evaluate learning and memory. Following FC 
      tests to evaluate inflammatory response (IR) and the permeability of barrier 
      systems, the hippocampus and colon were extracted, and feces were collected for 
      16 s rRNA. RESULTS: One week after surgery, surgery/anesthesia decreased the 
      frozen behavior. Cefazolin attenuated this declination but aggravated 
      postoperative freezing behavior 3 weeks after surgery. Probiotics ameliorated 
      surgery/anesthesia-induced memory deficits and perioperative cefazolin-induced 
      postoperative memory deficits 3 weeks after surgery. NLRP3, caspase-1, 
      Interleukin-1beta (IL-1beta), and Interleukin-18 (IL-18) levels were increased 1 week 
      after the hippocampus and colon surgery, which were attenuated by CY-09 and 
      probiotics, respectively. DISCUSSION: Probiotics could correct dysbacteria and IR 
      caused by surgery/anesthesia stress and cefazolin alone. These findings imply 
      that probiotics are an efficient and effective way of maintaining the balance of 
      gut microbiota, which may reduce NLRP3-related inflammation and alleviate PND.
CI  - Copyright (c) 2023 Zhang, Wu, Liu, Huang, Zhou and Liang.
FAU - Zhang, Tianyao
AU  - Zhang T
AD  - Department of Anesthesiology, West China Hospital of Sichuan University, Chengdu, 
      China.
AD  - Department of Anesthesiology, The First Affiliated Hospital of Chengdu Medical 
      College, Chengdu, China.
FAU - Wu, Xiaochu
AU  - Wu X
AD  - National Clinical Research Center for Geriatrics, West China Hospital, Sichuan 
      University, Chengdu, China.
FAU - Liu, Bin
AU  - Liu B
AD  - Department of Anesthesiology, West China Hospital of Sichuan University, Chengdu, 
      China.
AD  - Laboratory of Anesthesia and Critical Care Medicine, Translational Neuroscience 
      Center, West China Hospital of Sichuan University, Chengdu, China.
FAU - Huang, Han
AU  - Huang H
AD  - Department of Anesthesiology and Key Laboratory of Birth Defects and Related 
      Diseases of Women and Children, Ministry of Education, West China Second Hospital 
      of Sichuan University, Chengdu, Sichuan, China.
FAU - Zhou, Cheng
AU  - Zhou C
AD  - Department of Anesthesiology, West China Hospital of Sichuan University, Chengdu, 
      China.
AD  - Laboratory of Anesthesia and Critical Care Medicine, Translational Neuroscience 
      Center, West China Hospital of Sichuan University, Chengdu, China.
FAU - Liang, Peng
AU  - Liang P
AD  - Department of Anesthesiology, West China Hospital of Sichuan University, Chengdu, 
      China.
AD  - Day Surgery Center, General Practice Medical Center, West China Hospital, Sichuan 
      University and the Research Units of West China (2018RU012), Chinese Academy of 
      Medical Sciences, Chengdu, China.
LA  - eng
PT  - Journal Article
DEP - 20230427
PL  - Switzerland
TA  - Front Neurosci
JT  - Frontiers in neuroscience
JID - 101478481
PMC - PMC10174111
OTO - NOTNLM
OT  - NLRP3 inflammasome
OT  - cefazolin
OT  - gut dysbacteria
OT  - neuroinflammation
OT  - perioperative neurocognitive disorders
OT  - probiotics
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/05/14 13:10
MHDA- 2023/05/14 13:11
PMCR- 2023/01/01
CRDT- 2023/05/14 11:54
PHST- 2023/02/01 00:00 [received]
PHST- 2023/04/04 00:00 [accepted]
PHST- 2023/05/14 13:11 [medline]
PHST- 2023/05/14 13:10 [pubmed]
PHST- 2023/05/14 11:54 [entrez]
PHST- 2023/01/01 00:00 [pmc-release]
AID - 10.3389/fnins.2023.1156453 [doi]
PST - epublish
SO  - Front Neurosci. 2023 Apr 27;17:1156453. doi: 10.3389/fnins.2023.1156453. 
      eCollection 2023.