PMID- 37179751
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230920
IS  - 1598-2629 (Print)
IS  - 2092-6685 (Electronic)
IS  - 1598-2629 (Linking)
VI  - 23
IP  - 2
DP  - 2023 Apr
TI  - Comprehensive Analysis of Epstein-Barr Virus LMP2A-Specific CD8(+) and CD4(+) T 
      Cell Responses Restricted to Each HLA Class I and II Allotype Within an 
      Individual.
PG  - e17
LID - 10.4110/in.2023.23.e17 [doi]
LID - e17
AB  - Latent membrane protein 2A (LMP2A), a latent Ag commonly expressed in 
      Epstein-Barr virus (EBV)-infected host cells, is a target for adoptive T cell 
      therapy in EBV-associated malignancies. To define whether individual human 
      leukocyte antigen (HLA) allotypes are used preferentially in EBV-specific T 
      lymphocyte responses, LMP2A-specific CD8(+) and CD4(+) T cell responses in 50 
      healthy donors were analyzed by ELISPOT assay using artificial Ag-presenting 
      cells expressing a single allotype. CD8(+) T cell responses were significantly 
      higher than CD4(+) T cell responses. CD8(+) T cell responses were ranked from 
      highest to lowest in the order HLA-A, HLA-B, and HLA-C loci, and CD4(+) T cell 
      responses were ranked in the order HLA-DR, HLA-DP, and HLA-DQ loci. Among the 32 
      HLA class I and 56 HLA class II allotypes, 6 HLA-A, 7 HLA-B, 5 HLA-C, 10 HLA-DR, 
      2 HLA-DQ, and 2 HLA-DP allotypes showed T cell responses higher than 50 
      spot-forming cells (SFCs)/5x10(5) CD8(+) or CD4(+) T cells. Twenty-nine donors 
      (58%) showed a high T cell response to at least one allotype of HLA class I or 
      class II, and 4 donors (8%) had a high response to both HLA class I and class II 
      allotypes. Interestingly, we observed an inverse correlation between the 
      proportion of LMP2A-specific T cell responses and the frequency of HLA class I 
      and II allotypes. These data demonstrate the allele dominance of LMP2A-specific T 
      cell responses among HLA allotypes and their intra-individual dominance in 
      response to only a few allotypes in an individual, which may provide useful 
      information for genetic, pathogenic, and immunotherapeutic approaches to 
      EBV-associated diseases.
CI  - Copyright (c) 2023. The Korean Association of Immunologists.
FAU - Jo, Hyeong-A
AU  - Jo HA
AUID- ORCID: 0000-0003-2981-9071
AD  - Department of Microbiology, College of Medicine, The Catholic University of 
      Korea, Seoul 06591, Korea.
AD  - Department of Biomedicine and Health Sciences, College of Medicine, The Catholic 
      University of Korea, Seoul 06591, Korea.
FAU - Hyun, Seung-Joo
AU  - Hyun SJ
AD  - Department of Microbiology, College of Medicine, The Catholic University of 
      Korea, Seoul 06591, Korea.
AD  - Department of Biomedicine and Health Sciences, College of Medicine, The Catholic 
      University of Korea, Seoul 06591, Korea.
FAU - Hyun, You-Seok
AU  - Hyun YS
AUID- ORCID: 0000-0001-8572-2595
AD  - Department of Microbiology, College of Medicine, The Catholic University of 
      Korea, Seoul 06591, Korea.
AD  - Department of Biomedicine and Health Sciences, College of Medicine, The Catholic 
      University of Korea, Seoul 06591, Korea.
FAU - Lee, Yong-Hun
AU  - Lee YH
AD  - Department of Microbiology, College of Medicine, The Catholic University of 
      Korea, Seoul 06591, Korea.
AD  - Department of Biomedicine and Health Sciences, College of Medicine, The Catholic 
      University of Korea, Seoul 06591, Korea.
FAU - Kim, Sun-Mi
AU  - Kim SM
AD  - Hematopoietic Stem Cell Bank, College of Medicine, The Catholic University of 
      Korea, Seoul 06591, Korea.
FAU - Baek, In-Cheol
AU  - Baek IC
AUID- ORCID: 0000-0003-3630-3364
AD  - Hematopoietic Stem Cell Bank, College of Medicine, The Catholic University of 
      Korea, Seoul 06591, Korea.
FAU - Sohn, Hyun-Jung
AU  - Sohn HJ
AD  - Hematopoietic Stem Cell Bank, College of Medicine, The Catholic University of 
      Korea, Seoul 06591, Korea.
FAU - Kim, Tai-Gyu
AU  - Kim TG
AUID- ORCID: 0000-0002-2226-2138
AD  - Department of Microbiology, College of Medicine, The Catholic University of 
      Korea, Seoul 06591, Korea.
AD  - Department of Biomedicine and Health Sciences, College of Medicine, The Catholic 
      University of Korea, Seoul 06591, Korea.
AD  - Hematopoietic Stem Cell Bank, College of Medicine, The Catholic University of 
      Korea, Seoul 06591, Korea.
LA  - eng
PT  - Journal Article
DEP - 20221202
PL  - Korea (South)
TA  - Immune Netw
JT  - Immune network
JID - 101137270
PMC - PMC10166658
OTO - NOTNLM
OT  - CD8+ and CD4+ T cells
OT  - Dominant allotype
OT  - Epstein-Barr virus
OT  - HLA class I and II
OT  - Intra-individual dominance
OT  - LMP2A
COIS- Conflict of Interest: The authors declare no potential conflicts of interest.
EDAT- 2023/05/14 13:10
MHDA- 2023/05/14 13:11
PMCR- 2023/04/01
CRDT- 2023/05/14 11:57
PHST- 2022/08/05 00:00 [received]
PHST- 2022/10/26 00:00 [revised]
PHST- 2022/11/02 00:00 [accepted]
PHST- 2023/05/14 13:10 [pubmed]
PHST- 2023/05/14 13:11 [medline]
PHST- 2023/05/14 11:57 [entrez]
PHST- 2023/04/01 00:00 [pmc-release]
AID - 10.4110/in.2023.23.e17 [doi]
PST - epublish
SO  - Immune Netw. 2022 Dec 2;23(2):e17. doi: 10.4110/in.2023.23.e17. eCollection 2023 
      Apr.