PMID- 37180243
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230516
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 14
DP  - 2023
TI  - Streptococcus suis contributes to inguinal lymph node lesions in piglets after 
      highly pathogenic porcine reproductive and respiratory syndrome virus infection.
PG  - 1159590
LID - 10.3389/fmicb.2023.1159590 [doi]
LID - 1159590
AB  - The swine pathogens porcine reproductive and respiratory syndrome virus (PRRSV) 
      and Streptococcus suis have both been reported to cause damage to the immune 
      organs. Inguinal lymph node (ILN) injury has been reported in PRRSV-infected pigs 
      with secondary S. suis infection, but not much is known about the mechanism. In 
      this study, secondary S. suis infection after highly pathogenic (HP)-PRRSV 
      infection caused more severe clinical symptoms, mortality, and ILN lesions. 
      Histopathological lesions were seen in ILNs with a marked decrease in lymphocyte 
      numbers. Terminal deoxynucleotidyl transferase (TdT)-mediated de-oxyuridine 
      triphosphate (dUTP)-biotin nick end-labeling (TUNEL) assays revealed that 
      HP-PRRSV strain HuN4 alone induced ILN apoptosis, but dual-infection with S. suis 
      strain BM0806 induced greater levels of apoptosis. Besides, we found that some 
      HP-PRRSV-infected cells underwent apoptosis. Furthermore, anti-caspase-3 antibody 
      staining confirmed that ILN apoptosis was mainly induced by a caspase-dependent 
      pathway. Pyroptosis was also observed in HP-PRRSV-infected cells, and there was 
      more pyroptosis in piglets infected with HP-PRRSV alone compared with those with 
      secondary S. suis infection, and HP-PRRSV-infected cells underwent pyroptosis. 
      Altogether, this is the first report to identify pyroptosis in ILNs and which 
      signaling pathway is related to ILN apoptosis in single or dual-infected piglets. 
      These results contribute to a better understanding of the pathogenic mechanisms 
      during secondary S. suis infection.
CI  - Copyright (c) 2023 Wang, Xu, Yang, Zhang, Li, Tang, Wang, Leng, An and Cai.
FAU - Wang, Shujie
AU  - Wang S
AD  - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
AD  - Heilongjiang Provincial Key Laboratory of Veterinary Immunology, Harbin, China.
FAU - Xu, Min
AU  - Xu M
AD  - Sinopharm Animal Health Corporation Ltd., Wuhan, China.
FAU - Yang, Kongbin
AU  - Yang K
AD  - Neurosurgery Department, The Fifth Affiliated Hospital of Guangzhou Medical 
      University, Guangzhou, China.
FAU - Zhang, Ying
AU  - Zhang Y
AD  - Shandong Binzhou Animal Science and Veterinary Medicine Academy, Binzhou, China.
FAU - Li, Siqi
AU  - Li S
AD  - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
FAU - Tang, Yan-Dong
AU  - Tang YD
AD  - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
FAU - Wang, Jinliang
AU  - Wang J
AD  - Shandong Binzhou Animal Science and Veterinary Medicine Academy, Binzhou, China.
FAU - Leng, Chaoliang
AU  - Leng C
AD  - Henan Key Laboratory of Insect Biology in Funiu Mountain, Henan Provincial 
      Engineering Laboratory of Insects Bio-Reactor, China-UK-NYNU-RRes Joint 
      Laboratory of Insect Biology, Nanyang Normal University, Nanyang, China.
FAU - An, Tongqing
AU  - An T
AD  - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
FAU - Cai, Xuehui
AU  - Cai X
AD  - State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
LA  - eng
PT  - Journal Article
DEP - 20230427
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC10172469
OTO - NOTNLM
OT  - HP-PRRSV
OT  - Streptococcus suis
OT  - apoptosis
OT  - inguinal lymph nodes
OT  - pyroptosis
COIS- MX was employed by the company Sinopharm Animal Health Corporation Ltd., Wuhan, 
      China. The remaining authors declare that the research was conducted in the 
      absence of any commercial or financial relationships that could be construed as a 
      potential conflict of interest.
EDAT- 2023/05/14 19:13
MHDA- 2023/05/14 19:14
PMCR- 2023/04/27
CRDT- 2023/05/14 12:03
PHST- 2023/02/06 00:00 [received]
PHST- 2023/04/13 00:00 [accepted]
PHST- 2023/05/14 19:14 [medline]
PHST- 2023/05/14 19:13 [pubmed]
PHST- 2023/05/14 12:03 [entrez]
PHST- 2023/04/27 00:00 [pmc-release]
AID - 10.3389/fmicb.2023.1159590 [doi]
PST - epublish
SO  - Front Microbiol. 2023 Apr 27;14:1159590. doi: 10.3389/fmicb.2023.1159590. 
      eCollection 2023.