PMID- 37183919
OWN - NLM
STAT- MEDLINE
DCOM- 20230516
LR  - 20230516
IS  - 1940-4344 (Electronic)
IS  - 1940-4344 (Linking)
VI  - 25
IP  - 5
DP  - 2023
TI  - Effects of the Extraction Solvents on Dissolution Rate and Antioxidant Capacity 
      of Auricularia auricula (Agaricomycetes) Polysaccharides In Vitro and In Vivo.
PG  - 61-74
LID - 10.1615/IntJMedMushrooms.2023048033 [doi]
AB  - This paper reports the effects of solvents on the dissolution rate and 
      antioxidant capacity of Auricularia auricula polysaccharides (AAPs). The 
      ultra-low temperature combined with microwave extraction (UME) was used to 
      compare the dissolution rates and molecular weights of AAPs using deionized water 
      and deep eutectic solvents (DES) as solvents, respectively. Scanning electron 
      microscope (SEM) was used to observe the effects of water extract (AAPs-FW) and 
      DES extract (AAPs-FD) on the cell wall of A. auricula. The antioxidant capacity 
      of polysaccharide extracts in vitro was assessed by using various methods (DPPH, 
      ABTS, and hydroxyl radicals). In addition, in vivo oxidative stress was assessed 
      using Caenorhabditis elegans models. The extract yield of AAPs varied among the 
      extracts and was 19.58% +/- 0.56% in AAPs-FW. Whereas DES-UME increased the yield 
      of polysaccharides (AAPs-FD) by 9.81% in the extraction medium containing 
      triethylene glycol-choline chloride, under the optimum conditions of 60 min 
      freezing time, 350 W, and 90 s microwave time. The microstructure of the cell 
      wall shown by SEM was consistent with the results of polysaccharide yields. The 
      molecular weights of AAPs-FW and AAPs-FD were found to be 398.107 kDa and 89.099 
      kDa, respectively. The results demonstrated that AAPs-FD exhibited potent radical 
      scavenging activity against DPPH and a weaker scavenging ability for ABTS and OH 
      radicals compared to AAPs-FW. In addition, both polysaccharide extracts increased 
      the survival rate of C. elegans under methyl viologen induced oxidative stress at 
      specific concentrations (p < 0.05), and the antioxidant capacity of AAPs-FD was 
      higher than that of AAPs-FW at low concentrations (0.125 mg/mL). This indicated 
      that both polysaccharides had a protective effect against damage induced by 
      intracellular free radical generators (methyl viologen).
FAU - Yao, Jing
AU  - Yao J
AD  - Liang Xin College, China Jiliang University, Hangzhou, Zhejiang 310018, People's 
      Republic of China.
FAU - Zeng, Jiang-Ying
AU  - Zeng JY
AD  - College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang Province, 
      310018, P.R. China.
FAU - Tang, Yu-Xuan
AU  - Tang YX
AD  - College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang Province, 
      310018, P.R. China.
FAU - Cheng, Yi-Rui
AU  - Cheng YR
AD  - College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang Province, 
      310018, P.R. China.
FAU - Li, Tian-Yuan
AU  - Li TY
AD  - Liang Xin College, China Jiliang University, Hangzhou, Zhejiang Province, 310018, 
      P.R. China.
FAU - Yang, Jun
AU  - Yang J
AD  - College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang Province, 
      310018, P.R. China.
FAU - Zhang, Yong-Jun
AU  - Zhang YJ
AD  - College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang 310018, 
      People's Republic of China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Int J Med Mushrooms
JT  - International journal of medicinal mushrooms
JID - 100886202
RN  - 0 (Antioxidants)
RN  - 28752-68-3 (2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid)
RN  - 0 (Solvents)
RN  - PLG39H7695 (Paraquat)
RN  - 0 (Polysaccharides)
RN  - 059QF0KO0R (Water)
RN  - Auricularia auricula-judae
SB  - IM
MH  - Animals
MH  - *Antioxidants/pharmacology/chemistry
MH  - Solvents/pharmacology
MH  - Caenorhabditis elegans
MH  - Solubility
MH  - Paraquat/pharmacology
MH  - *Basidiomycota/chemistry
MH  - Polysaccharides/pharmacology/chemistry
MH  - Water
EDAT- 2023/05/15 13:06
MHDA- 2023/05/16 06:42
CRDT- 2023/05/15 06:43
PHST- 2023/05/16 06:42 [medline]
PHST- 2023/05/15 13:06 [pubmed]
PHST- 2023/05/15 06:43 [entrez]
AID - 3eb4f7215a4b8ea4,147680683708e1ee [pii]
AID - 10.1615/IntJMedMushrooms.2023048033 [doi]
PST - ppublish
SO  - Int J Med Mushrooms. 2023;25(5):61-74. doi: 10.1615/IntJMedMushrooms.2023048033.