PMID- 37186777
OWN - NLM
STAT- MEDLINE
DCOM- 20230901
LR  - 20240315
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Print)
IS  - 0032-0889 (Linking)
VI  - 193
IP  - 1
DP  - 2023 Aug 31
TI  - Extrachromosomal circular DNA-mediated spread of herbicide resistance in 
      interspecific hybrids of pigweed.
PG  - 229-233
LID - 10.1093/plphys/kiad281 [doi]
AB  - Extrachromosomal circular DNAs (eccDNAs) are found in many eukaryotic organisms. 
      EccDNA-powered copy number variation plays diverse roles, from oncogenesis in 
      humans to herbicide resistance in crop weeds. Here, we report interspecific 
      eccDNA flow and its dynamic behavior in soma cells of natural populations and F1 
      hybrids of Amaranthus sp. The glyphosate-resistance (GR) trait is controlled by 
      eccDNA-based amplification harboring the 5-enolpyruvylshikimate-3-phosphate 
      synthase (EPSPS) gene (eccDNA replicon), the molecular target of glyphosate. We 
      documented pollen-mediated transfer of eccDNA in experimental hybrids between 
      glyphosate-susceptible Amaranthus tuberculatus and GR Amaranthus palmeri. 
      Experimental hybridization and fluorescence in situ hybridization (FISH) analysis 
      revealed that the eccDNA replicon in Amaranthus spinosus derived from GR A. 
      palmeri by natural hybridization. FISH analysis also revealed random chromosome 
      anchoring and massive eccDNA replicon copy number variation in soma cells of 
      weedy hybrids. The results suggest that eccDNAs are inheritable across compatible 
      species, contributing to genome plasticity and rapid adaptive evolution.
CI  - (c) The Author(s) 2023. Published by Oxford University Press on behalf of American 
      Society of Plant Biologists.
FAU - Koo, Dal-Hoe
AU  - Koo DH
AUID- ORCID: 0000-0003-4578-8519
AD  - Wheat Genetics Resource Center and Department of Plant Pathology, Kansas State 
      University, Manhattan, KS 66506, USA.
FAU - Sathishraj, Rajendran
AU  - Sathishraj R
AUID- ORCID: 0000-0002-4442-6850
AD  - Wheat Genetics Resource Center and Department of Plant Pathology, Kansas State 
      University, Manhattan, KS 66506, USA.
FAU - Nakka, Sridevi
AU  - Nakka S
AD  - Heartland Plant Innovations Inc., Manhattan, KS 66506, USA.
FAU - Ju, Yoonha
AU  - Ju Y
AUID- ORCID: 0000-0001-9792-2041
AD  - Wheat Genetics Resource Center and Department of Plant Pathology, Kansas State 
      University, Manhattan, KS 66506, USA.
FAU - Nandula, Vijay K
AU  - Nandula VK
AUID- ORCID: 0000-0001-5618-7634
AD  - National Institute of Food and Agriculture, USDA, Kansas City, MO 64105, USA.
AD  - Crop Production Systems Research Unit, USDA-ARS, Stoneville, MS 38776, USA.
FAU - Jugulam, Mithila
AU  - Jugulam M
AUID- ORCID: 0000-0003-2065-9067
AD  - Department of Agronomy, Kansas State University, Manhattan, KS 66506, USA.
FAU - Friebe, Bernd
AU  - Friebe B
AUID- ORCID: 0000-0002-5419-1565
AD  - Wheat Genetics Resource Center and Department of Plant Pathology, Kansas State 
      University, Manhattan, KS 66506, USA.
FAU - Gill, Bikram S
AU  - Gill BS
AUID- ORCID: 0000-0003-4510-9459
AD  - Wheat Genetics Resource Center and Department of Plant Pathology, Kansas State 
      University, Manhattan, KS 66506, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
RN  - 9007-49-2 (DNA)
RN  - 0 (DNA, Circular)
RN  - 0 (Herbicides)
SB  - IM
CIN - Plant Physiol. 2023 Aug 31;193(1):172-173. PMID: 37394915
MH  - Humans
MH  - *Amaranthus/genetics
MH  - Herbicide Resistance/genetics
MH  - DNA Copy Number Variations
MH  - In Situ Hybridization, Fluorescence
MH  - DNA
MH  - DNA, Circular
MH  - *Herbicides/pharmacology
PMC - PMC10469533
COIS- Conflict of interest statement. None declared.
EDAT- 2023/05/15 19:13
MHDA- 2023/09/01 06:43
PMCR- 2023/05/15
CRDT- 2023/05/15 14:41
PHST- 2023/03/01 00:00 [received]
PHST- 2023/04/18 00:00 [accepted]
PHST- 2023/09/01 06:43 [medline]
PHST- 2023/05/15 19:13 [pubmed]
PHST- 2023/05/15 14:41 [entrez]
PHST- 2023/05/15 00:00 [pmc-release]
AID - 7163151 [pii]
AID - kiad281 [pii]
AID - 10.1093/plphys/kiad281 [doi]
PST - ppublish
SO  - Plant Physiol. 2023 Aug 31;193(1):229-233. doi: 10.1093/plphys/kiad281.