PMID- 37187181
OWN - NLM
STAT- MEDLINE
DCOM- 20230911
LR  - 20230911
IS  - 1439-4286 (Electronic)
IS  - 0018-5043 (Linking)
VI  - 55
IP  - 9
DP  - 2023 Sep
TI  - LncRNA LINC01018 Screens Type 2 Diabetes Mellitus and Regulates beta Cell Function 
      Through Modulating miR-499a-5p.
PG  - 642-648
LID - 10.1055/a-2077-5177 [doi]
AB  - Type 2 diabetes mellitus (T2DM) is characterized by hyperglycemia, which 
      seriously endangers human health. The dysregulation of lncRNA LINC01018 in T2DM 
      has been noticed in previous studies, but whether it served as a biomarker lacks 
      validation. This study aimed to confirm the abnormal expression of LINC01018 in 
      T2DM and reveals its specific function in regulating pancreatic beta cell function. 
      This study enrolled 77 T2DM patients and 41 healthy individuals and compared the 
      plasma LINC01018 levels between two groups using PCR. The pancreatic beta cell was 
      induced with 25 mM glucose to mimic cell injury during T2DM. The effects of 
      LINC01018 on beta cell proliferation, dedifferentiation, and insulin production were 
      evaluated by CCK8, western blotting, and ELISA. Moreover, the involvement of 
      miR-499a-5p was also evaluated with luciferase reporter assay. Increased plasma 
      LINC01018 was observed in T2DM patients compared with healthy individuals, which 
      discriminates patients with high sensitivity and specificity. Upregulated 
      LINC01018 was associated with patients' fasting blood glucose and weight loss. 
      High glucose induced the increasing LINC01018 in pancreatic islet beta cells and 
      suppressed cell proliferation, insulin secretion, and promoted cell 
      dedifferentiation. Silencing LINC01018 could alleviate the impaired function of beta 
      cells by high glucose, which was reversed by the knockdown by miR-499a-5p. 
      Upregulated LINC01018 served as a potential diagnostic biomarker for T2DM and 
      alleviated high glucose-induced beta cell dysfunction via negatively modulating 
      miR-499a-5p.
CI  - Thieme. All rights reserved.
FAU - Liu, Li
AU  - Liu L
AUID- ORCID: 0000-0003-4594-3937
AD  - Department of General Practice, Affiliated Hospital of Panzhihua University, 
      Panzhihua, China.
FAU - Li, Yuan
AU  - Li Y
AD  - Department of General Practice, Affiliated Hospital of Panzhihua University, 
      Panzhihua, China.
FAU - Zhang, Xiaoqian
AU  - Zhang X
AD  - Department of General Practice, Affiliated Hospital of Panzhihua University, 
      Panzhihua, China.
LA  - eng
PT  - Journal Article
DEP - 20230515
PL  - Germany
TA  - Horm Metab Res
JT  - Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et 
      metabolisme
JID - 0177722
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Humans
MH  - *MicroRNAs/genetics/metabolism
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - *Diabetes Mellitus, Type 2/genetics/metabolism
MH  - *Insulin-Secreting Cells/metabolism
MH  - Glucose/pharmacology/metabolism
COIS- The authors declare that they have no conflict of interest.
EDAT- 2023/05/16 01:08
MHDA- 2023/09/11 06:42
CRDT- 2023/05/15 18:52
PHST- 2023/09/11 06:42 [medline]
PHST- 2023/05/16 01:08 [pubmed]
PHST- 2023/05/15 18:52 [entrez]
AID - 10.1055/a-2077-5177 [doi]
PST - ppublish
SO  - Horm Metab Res. 2023 Sep;55(9):642-648. doi: 10.1055/a-2077-5177. Epub 2023 May 
      15.