PMID- 37188710 OWN - NLM STAT- MEDLINE DCOM- 20230517 LR - 20230606 IS - 2045-2322 (Electronic) IS - 2045-2322 (Linking) VI - 13 IP - 1 DP - 2023 May 15 TI - Effect of Bacillus subtilis on mechanical and self-healing properties in mortar with different crack widths and curing conditions. PG - 7844 LID - 10.1038/s41598-023-34837-x [doi] LID - 7844 AB - This research aimed to investigate the effectiveness of Bacillus subtilis (B. subtilis) in self-healing cracks in concrete and enhancing concrete strength through microbial induced calcium carbonate precipitation (MICP). The study evaluated the ability of the mortar to cover cracks within 28 days, taking into account the width of the crack, and observed the recovery of strength after self-healing. The use of microencapsulated endospores of B. subtilis was also examined for its impact on the strength of concrete. The compressive, splitting tensile, and flexural strengths of normal mortar were compared to those of biological mortar, and it was found that biological mortar had a higher strength capacity. Microstructure analysis using SEM and EDS showed that bacterial growth increased calcium production, contributing to the improved mechanical properties of the bio-mortar. CI - (c) 2023. The Author(s). FAU - Yamasamit, Nattapong AU - Yamasamit N AD - Department of Civil Engineering, Faculty of Engineering, Thammasat School of Engineering, Thammasat University, Pathum Thani, Thailand. FAU - Sangkeaw, Panisa AU - Sangkeaw P AD - Department of Civil Engineering, Faculty of Engineering, Thammasat School of Engineering, Thammasat University, Pathum Thani, Thailand. FAU - Jitchaijaroen, Wittaya AU - Jitchaijaroen W AD - Department of Civil Engineering, Faculty of Engineering, Thammasat School of Engineering, Thammasat University, Pathum Thani, Thailand. FAU - Thongchom, Chanachai AU - Thongchom C AD - Department of Civil Engineering, Faculty of Engineering, Thammasat School of Engineering, Thammasat University, Pathum Thani, Thailand. tchanach@engr.tu.ac.th. FAU - Keawsawasvong, Suraparb AU - Keawsawasvong S AD - Department of Civil Engineering, Faculty of Engineering, Thammasat School of Engineering, Thammasat University, Pathum Thani, Thailand. FAU - Kamchoom, Viroon AU - Kamchoom V AD - Excellent Centre for Green and Sustainable Infrastructure, Department of Civil Engineering, School of Engineering, King Mongkut's Institute of Technology Ladkrabang (KMITL), Bangkok, Thailand. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20230515 PL - England TA - Sci Rep JT - Scientific reports JID - 101563288 RN - H0G9379FGK (Calcium Carbonate) SB - IM MH - *Bacillus subtilis/chemistry MH - *Construction Materials/microbiology MH - Calcium Carbonate/chemistry MH - Spores, Bacterial PMC - PMC10185526 COIS- The authors declare no competing interests. EDAT- 2023/05/16 01:09 MHDA- 2023/05/17 06:42 PMCR- 2023/05/15 CRDT- 2023/05/15 23:17 PHST- 2023/02/27 00:00 [received] PHST- 2023/05/09 00:00 [accepted] PHST- 2023/05/17 06:42 [medline] PHST- 2023/05/16 01:09 [pubmed] PHST- 2023/05/15 23:17 [entrez] PHST- 2023/05/15 00:00 [pmc-release] AID - 10.1038/s41598-023-34837-x [pii] AID - 34837 [pii] AID - 10.1038/s41598-023-34837-x [doi] PST - epublish SO - Sci Rep. 2023 May 15;13(1):7844. doi: 10.1038/s41598-023-34837-x.