PMID- 37189668
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230519
IS  - 2227-9059 (Print)
IS  - 2227-9059 (Electronic)
IS  - 2227-9059 (Linking)
VI  - 11
IP  - 4
DP  - 2023 Mar 29
TI  - Short-Term Growth Hormone Administration Mediates Hepatic Fatty Acid Uptake and 
      De Novo Lipogenesis Gene Expression in Obese Rats.
LID - 10.3390/biomedicines11041050 [doi]
LID - 1050
AB  - Obesity has been linked to metabolic syndrome, type 2 diabetes, and non-alcoholic 
      fatty liver disease (NAFLD). Obesity causes a decrease in growth hormone (GH) 
      levels and an increase in insulin levels. Long-term GH treatment increased 
      lipolytic activity as opposed to decreasing insulin sensitivity. Nonetheless, it 
      is possible that short-term GH administration had no impact on insulin 
      sensitivity. In this study, the effect of short-term GH administration on liver 
      lipid metabolism and the effector molecules of GH and insulin receptors were 
      investigated in diet-induced obesity (DIO) rats. Recombinant human GH (1 mg/kg) 
      was then administered for 3 days. Livers were collected to determine the hepatic 
      mRNA expression and protein levels involved in lipid metabolism. The expression 
      of GH and insulin receptor effector proteins was investigated. In DIO rats, 
      short-term GH administration significantly reduced hepatic fatty acid synthase 
      (FASN) and cluster of differentiation 36 (CD36) mRNA expression while increasing 
      carnitine palmitoyltransferase 1A (CPT1A) mRNA expression. Short-term GH 
      administration reduced hepatic FAS protein levels and downregulated gene 
      transcription of hepatic fatty acid uptake and lipogenesis, while increasing 
      fatty acid oxidation in DIO rats. DIO rats had lower hepatic JAK2 protein levels 
      but higher IRS-1 levels than control rats due to hyperinsulinemia. Our findings 
      suggest that short-term GH supplementation improves liver lipid metabolism and 
      may slow the progression of NAFLD, where GH acts as the transcriptional regulator 
      of related genes.
FAU - Likitnukul, Sutharinee
AU  - Likitnukul S
AUID- ORCID: 0000-0002-2305-9107
AD  - Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok 
      10400, Thailand.
FAU - Thammacharoen, Sumpun
AU  - Thammacharoen S
AUID- ORCID: 0000-0002-5500-1639
AD  - Department of Physiology, Faculty of Veterinary Science, Chulalongkorn 
      University, Bangkok 10330, Thailand.
FAU - Sriwatananukulkit, Orada
AU  - Sriwatananukulkit O
AD  - Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok 
      10400, Thailand.
FAU - Duangtha, Chanathip
AU  - Duangtha C
AD  - Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok 
      10400, Thailand.
FAU - Hemstapat, Ruedee
AU  - Hemstapat R
AUID- ORCID: 0000-0003-4939-7937
AD  - Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok 
      10400, Thailand.
FAU - Sunrat, Chotchanit
AU  - Sunrat C
AD  - Department of Physiology, Faculty of Science, Mahidol University, Bangkok 10400, 
      Thailand.
FAU - Mangmool, Supachoke
AU  - Mangmool S
AUID- ORCID: 0000-0003-3223-1551
AD  - Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok 
      10400, Thailand.
FAU - Pinthong, Darawan
AU  - Pinthong D
AD  - Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok 
      10400, Thailand.
LA  - eng
GR  - The Central Instrument Facility (CIF) and Center of Nanoimaging (CNI), Faculty of 
      Science, Mahidol University/
GR  - Mahidol University/
PT  - Journal Article
DEP - 20230329
PL  - Switzerland
TA  - Biomedicines
JT  - Biomedicines
JID - 101691304
PMC - PMC10135739
OTO - NOTNLM
OT  - DIO rats
OT  - GH
OT  - NAFLD
OT  - hepatic lipid metabolism
OT  - insulin resistance
COIS- The authors declare no conflict of interest.
EDAT- 2023/05/16 06:42
MHDA- 2023/05/16 06:43
PMCR- 2023/03/29
CRDT- 2023/05/16 01:10
PHST- 2023/02/20 00:00 [received]
PHST- 2023/03/22 00:00 [revised]
PHST- 2023/03/23 00:00 [accepted]
PHST- 2023/05/16 06:43 [medline]
PHST- 2023/05/16 06:42 [pubmed]
PHST- 2023/05/16 01:10 [entrez]
PHST- 2023/03/29 00:00 [pmc-release]
AID - biomedicines11041050 [pii]
AID - biomedicines-11-01050 [pii]
AID - 10.3390/biomedicines11041050 [doi]
PST - epublish
SO  - Biomedicines. 2023 Mar 29;11(4):1050. doi: 10.3390/biomedicines11041050.