PMID- 37189776
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230519
IS  - 2227-9059 (Print)
IS  - 2227-9059 (Electronic)
IS  - 2227-9059 (Linking)
VI  - 11
IP  - 4
DP  - 2023 Apr 12
TI  - Encapsulation of Human Umbilical Cord Mesenchymal Stem Cells in LunaGel 
      Photocrosslinkable Extracellular Matrix and Subcutaneous Transplantation in Mice.
LID - 10.3390/biomedicines11041158 [doi]
LID - 1158
AB  - Stem cells have significant potential in regenerative medicines. However, a major 
      issue with implanting stem cells in the regeneration of new tissue is the methods 
      to implant them and cell viability and functions before and after implantation. 
      Here we developed a simple yet effective method that used photo-crosslinkable 
      gelatin-based hydrogel (LunaGel(TM)) as a scaffold for the encapsulation, 
      expansion, and eventually, transplantation of human umbilical cord-derived 
      mesenchymal stem cells (hUC-MSCs) into mice subcutaneously. We demonstrated the 
      proliferation and maintenance of the original expression of mesenchymal stem cell 
      markers as well as the ability to differentiate into mesoderm-derived cells. The 
      hydrogel was highly stable with no signs of degradation after 20 days in PBS. The 
      hUC-MSCs remained viable after transplantation into mice's subcutaneous pockets 
      and migrated to integrate with the surrounding tissues. We showed a collagen-rich 
      layer surrounding the transplanted cell-laden scaffold indicating the effects of 
      growth factors secreted by the hUC-MSCs. A connective tissue layer was found 
      between the implanted cell-laden scaffold and the collagen layer, and 
      immunohistochemical staining results suggested that this tissue was derived from 
      the MSCs which migrated from within the scaffold. The results, thus, also 
      suggested a protective effect the scaffold has on the encapsulated cells from the 
      antibodies and cytotoxic cells of the host immune system.
FAU - Pham, Truc Le-Buu
AU  - Pham TL
AUID- ORCID: 0000-0001-7601-3527
AD  - Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam.
FAU - Nguyen, Dang Phu-Hai
AU  - Nguyen DP
AUID- ORCID: 0000-0002-7232-099X
AD  - Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam.
FAU - Luu, Thao Thi-Thu
AU  - Luu TT
AUID- ORCID: 0009-0006-8369-7676
AD  - Histology-Embryology-Pathology Department, Faculty of Medicine, University of 
      Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam.
FAU - Nguyen, Luong Si
AU  - Nguyen LS
AD  - Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam.
FAU - Binh, Nguyen Trong
AU  - Binh NT
AD  - Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam.
FAU - Nguyen, Quan Dang
AU  - Nguyen QD
AD  - Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam.
FAU - Tran, Phong Anh
AU  - Tran PA
AD  - Interface Science and Materials Engineering Group, School of Mechanical, Medical 
      and Process Engineering, Faculty of Engineering, Queensland University of 
      Technology, Brisbane City, QLD 4000, Australia.
LA  - eng
PT  - Journal Article
DEP - 20230412
PL  - Switzerland
TA  - Biomedicines
JT  - Biomedicines
JID - 101691304
PMC - PMC10135450
OTO - NOTNLM
OT  - LunaGel
OT  - cell-laden scaffold
OT  - mesenchymal stem cells
OT  - regenerative medicine
OT  - umbilical cord
COIS- The authors declare no conflict of interest.
EDAT- 2023/05/16 06:42
MHDA- 2023/05/16 06:43
PMCR- 2023/04/12
CRDT- 2023/05/16 01:11
PHST- 2023/02/26 00:00 [received]
PHST- 2023/04/03 00:00 [revised]
PHST- 2023/04/05 00:00 [accepted]
PHST- 2023/05/16 06:43 [medline]
PHST- 2023/05/16 06:42 [pubmed]
PHST- 2023/05/16 01:11 [entrez]
PHST- 2023/04/12 00:00 [pmc-release]
AID - biomedicines11041158 [pii]
AID - biomedicines-11-01158 [pii]
AID - 10.3390/biomedicines11041158 [doi]
PST - epublish
SO  - Biomedicines. 2023 Apr 12;11(4):1158. doi: 10.3390/biomedicines11041158.