PMID- 37202804
OWN - NLM
STAT- MEDLINE
DCOM- 20230522
LR  - 20230525
IS  - 1743-8977 (Electronic)
IS  - 1743-8977 (Linking)
VI  - 20
IP  - 1
DP  - 2023 May 18
TI  - Placental-fetal distribution of carbon particles in a pregnant rabbit model after 
      repeated exposure to diluted diesel engine exhaust.
PG  - 20
LID - 10.1186/s12989-023-00531-z [doi]
LID - 20
AB  - BACKGROUND: Airborne pollution particles have been shown to translocate from the 
      mother's lung to the fetal circulation, but their distribution and internal 
      placental-fetal tissue load remain poorly explored. Here, we investigated the 
      placental-fetal load and distribution of diesel engine exhaust particles during 
      gestation under controlled exposure conditions using a pregnant rabbit model. 
      Pregnant dams were exposed by nose-only inhalation to either clean air (controls) 
      or diluted and filtered diesel engine exhaust (1 mg/m(3)) for 2 h/day, 5 
      days/week, from gestational day (GD) 3 to GD27. At GD28, placental and fetal 
      tissues (i.e., heart, kidney, liver, lung and gonads) were collected for biometry 
      and to study the presence of carbon particles (CPs) using white light generation 
      by carbonaceous particles under femtosecond pulsed laser illumination. RESULTS: 
      CPs were detected in the placenta, fetal heart, kidney, liver, lung and gonads in 
      significantly higher amounts in exposed rabbits compared with controls. Through 
      multiple factor analysis, we were able to discriminate the diesel engine exposed 
      pregnant rabbits from the control group taking all variables related to 
      fetoplacental biometry and CP load into consideration. Our findings did not 
      reveal a sex effect, yet a potential interaction effect might be present between 
      exposure and fetal sex. CONCLUSIONS: The results confirmed the translocation of 
      maternally inhaled CPs from diesel engine exhaust to the placenta which could be 
      detected in fetal organs during late-stage pregnancy. The exposed can be clearly 
      discriminated from the control group with respect to fetoplacental biometry and 
      CP load. The differential particle load in the fetal organs may contribute to the 
      effects on fetoplacental biometry and to the malprogramming of the fetal 
      phenotype with long-term effects later in life.
CI  - (c) 2023. The Author(s).
FAU - Bongaerts, Eva
AU  - Bongaerts E
AD  - Centre for Environmental Sciences, Hasselt University, Agoralaan Building D, 
      3590, Diepenbeek, Belgium.
FAU - Nawrot, Tim S
AU  - Nawrot TS
AD  - Centre for Environmental Sciences, Hasselt University, Agoralaan Building D, 
      3590, Diepenbeek, Belgium. tim.nawrot@uhasselt.be.
AD  - Department of Public Health and Primary Care, KU Leuven, Kapucijnenvoer 35 blok 
      d-box 7001, Leuven, 3000, Belgium. tim.nawrot@uhasselt.be.
FAU - Wang, Congrong
AU  - Wang C
AD  - Centre for Environmental Sciences, Hasselt University, Agoralaan Building D, 
      3590, Diepenbeek, Belgium.
FAU - Ameloot, Marcel
AU  - Ameloot M
AD  - Biomedical Research Institute, Hasselt University, Agoralaan Building C, 
      Diepenbeek, 3590, Belgium.
FAU - Bove, Hannelore
AU  - Bove H
AD  - Centre for Environmental Sciences, Hasselt University, Agoralaan Building D, 
      3590, Diepenbeek, Belgium.
FAU - Roeffaers, Maarten Bj
AU  - Roeffaers MB
AD  - Department of Microbial and Molecular Systems, KU Leuven, Celestijnenlaan, 
      Leuven, 200F-box 2454, 3001, Belgium.
FAU - Chavatte-Palmer, Pascale
AU  - Chavatte-Palmer P
AD  - Universite Paris-Saclay, UVSQ, INRAE, BREED, Jouy-en-Josas, 78350, France.
AD  - Ecole Nationale Veterinaire d'Alfort, BREED, Misons-Alfort, 94700, France.
FAU - Couturier-Tarrade, Anne
AU  - Couturier-Tarrade A
AD  - Universite Paris-Saclay, UVSQ, INRAE, BREED, Jouy-en-Josas, 78350, France.
AD  - Ecole Nationale Veterinaire d'Alfort, BREED, Misons-Alfort, 94700, France.
FAU - Cassee, Flemming R
AU  - Cassee FR
AD  - National Institute for Public Health and the Environment, RIVM, PObox1, 
      Bilthoven, 3720 BA, the Netherlands.
AD  - Institute for Risk Assessment Sciences, Division Toxicology, Utrecht University, 
      Utrecht, the Netherlands.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230518
PL  - England
TA  - Part Fibre Toxicol
JT  - Particle and fibre toxicology
JID - 101236354
RN  - 0 (Vehicle Emissions)
RN  - 7440-44-0 (Carbon)
SB  - IM
MH  - Animals
MH  - Pregnancy
MH  - Rabbits
MH  - Female
MH  - *Placenta
MH  - *Vehicle Emissions/toxicity
MH  - Carbon/toxicity
MH  - Lung
MH  - Liver
PMC - PMC10193698
OTO - NOTNLM
OT  - Airborne pollution
OT  - Diesel exhaust
OT  - Gestational exposure
OT  - Label-free detection
COIS- T.S.N., M.A., H.B. and M.B.J.R. declare competing financial interest: aspects of 
      the work mentioned in the paper are the subject of an awarded patent (Method for 
      detecting or quantifying carbon black and/or black carbon particles, reference 
      codes: EP3403068B1 and US11002679B2) filed by Hasselt University (Hasselt, 
      Belgium) and KU Leuven (Leuven, Belgium). The remaining authors declare no 
      competing interests.
EDAT- 2023/05/19 01:04
MHDA- 2023/05/22 06:42
PMCR- 2023/05/18
CRDT- 2023/05/18 23:34
PHST- 2022/11/24 00:00 [received]
PHST- 2023/05/06 00:00 [accepted]
PHST- 2023/05/22 06:42 [medline]
PHST- 2023/05/19 01:04 [pubmed]
PHST- 2023/05/18 23:34 [entrez]
PHST- 2023/05/18 00:00 [pmc-release]
AID - 10.1186/s12989-023-00531-z [pii]
AID - 531 [pii]
AID - 10.1186/s12989-023-00531-z [doi]
PST - epublish
SO  - Part Fibre Toxicol. 2023 May 18;20(1):20. doi: 10.1186/s12989-023-00531-z.