PMID- 37206216
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230522
IS  - 2380-6761 (Print)
IS  - 2380-6761 (Electronic)
IS  - 2380-6761 (Linking)
VI  - 8
IP  - 3
DP  - 2023 May
TI  - Gellan gum spongy-like hydrogel-based dual antibiotic therapy for infected 
      diabetic wounds.
PG  - e10504
LID - 10.1002/btm2.10504 [doi]
LID - e10504
AB  - Diabetic foot infection (DFI) is an important cause of morbidity and mortality. 
      Antibiotics are fundamental for treating DFI, although bacterial biofilm 
      formation and associated pathophysiology can reduce their effectiveness. 
      Additionally, antibiotics are often associated with adverse reactions. Hence, 
      improved antibiotic therapies are required for safer and effective DFI 
      management. On this regard, drug delivery systems (DDSs) constitute a promising 
      strategy. We propose a gellan gum (GG)-based spongy-like hydrogel as a topical 
      and controlled DDS of vancomycin and clindamycin, for an improved dual antibiotic 
      therapy against methicillin-resistant Staphylococcus aureus (MRSA) in DFI. The 
      developed DDS presents suitable features for topical application, while promoting 
      the controlled release of both antibiotics, resulting in a significant reduction 
      of in vitro antibiotic-associated cytotoxicity without compromising antibacterial 
      activity. The therapeutic potential of this DDS was further corroborated in vivo, 
      in a diabetic mouse model of MRSA-infected wounds. A single DDS administration 
      allowed a significant bacterial burden reduction in a short period of time, 
      without exacerbating host inflammatory response. Taken together, these results 
      suggest that the proposed DDS represents a promising strategy for the topical 
      treatment of DFI, potentially overcoming limitations associated with systemic 
      antibiotic administration and minimizing the frequency of administration.
CI  - (c) 2023 The Authors. Bioengineering & Translational Medicine published by Wiley 
      Periodicals LLC on behalf of American Institute of Chemical Engineers.
FAU - Mendes, Ana Isabel
AU  - Mendes AI
AUID- ORCID: 0000-0003-4724-0784
AD  - Life and Health Sciences Research Institute (ICVS) School of Medicine University 
      of Minho Braga Portugal.
AD  - ICVS/3B's-PT Government Associate Laboratory Braga/Guimaraes Portugal.
FAU - Fraga, Alexandra Gabriel
AU  - Fraga AG
AUID- ORCID: 0000-0003-2029-1629
AD  - Life and Health Sciences Research Institute (ICVS) School of Medicine University 
      of Minho Braga Portugal.
AD  - ICVS/3B's-PT Government Associate Laboratory Braga/Guimaraes Portugal.
FAU - Peixoto, Maria Joao
AU  - Peixoto MJ
AUID- ORCID: 0000-0002-4739-3409
AD  - Life and Health Sciences Research Institute (ICVS) School of Medicine University 
      of Minho Braga Portugal.
AD  - ICVS/3B's-PT Government Associate Laboratory Braga/Guimaraes Portugal.
FAU - Aroso, Ivo
AU  - Aroso I
AUID- ORCID: 0000-0002-0543-548X
AD  - ICVS/3B's-PT Government Associate Laboratory Braga/Guimaraes Portugal.
AD  - 3B's Research Group, I3Bs - Research Institute on Biomaterials, Biodegradables 
      and Biomimetics Headquarters of the European Institute of Excellence on Tissue 
      Engineering and Regenerative Medicine University of Minho Guimaraes Portugal.
FAU - Longatto-Filho, Adhemar
AU  - Longatto-Filho A
AUID- ORCID: 0000-0002-5779-9752
AD  - Life and Health Sciences Research Institute (ICVS) School of Medicine University 
      of Minho Braga Portugal.
AD  - ICVS/3B's-PT Government Associate Laboratory Braga/Guimaraes Portugal.
AD  - Molecular Oncology Research Center Barretos Cancer Hospital Barretos Sao Paulo 
      Brazil.
AD  - Laboratory of Medical Investigation (LIM) 14 Hospital das Clinicas da Faculdade 
      de Medicina da Universidade de Sao Paulo Sao Paulo Brazil.
FAU - Marques, Alexandra Pinto
AU  - Marques AP
AUID- ORCID: 0000-0002-3222-0310
AD  - ICVS/3B's-PT Government Associate Laboratory Braga/Guimaraes Portugal.
AD  - 3B's Research Group, I3Bs - Research Institute on Biomaterials, Biodegradables 
      and Biomimetics Headquarters of the European Institute of Excellence on Tissue 
      Engineering and Regenerative Medicine University of Minho Guimaraes Portugal.
FAU - Pedrosa, Jorge
AU  - Pedrosa J
AUID- ORCID: 0000-0002-8204-4888
AD  - Life and Health Sciences Research Institute (ICVS) School of Medicine University 
      of Minho Braga Portugal.
AD  - ICVS/3B's-PT Government Associate Laboratory Braga/Guimaraes Portugal.
LA  - eng
PT  - Journal Article
DEP - 20230321
PL  - United States
TA  - Bioeng Transl Med
JT  - Bioengineering & translational medicine
JID - 101689146
PMC - PMC10189450
OTO - NOTNLM
OT  - MRSA infection
OT  - antibiotics
OT  - diabetic ulcers
OT  - hydrogel
OT  - topical delivery
COIS- The authors have no conflicts of interest to declare.
EDAT- 2023/05/19 19:14
MHDA- 2023/05/19 19:15
PMCR- 2023/03/21
CRDT- 2023/05/19 15:38
PHST- 2022/11/14 00:00 [received]
PHST- 2023/02/15 00:00 [revised]
PHST- 2023/02/27 00:00 [accepted]
PHST- 2023/05/19 19:15 [medline]
PHST- 2023/05/19 19:14 [pubmed]
PHST- 2023/05/19 15:38 [entrez]
PHST- 2023/03/21 00:00 [pmc-release]
AID - BTM210504 [pii]
AID - 10.1002/btm2.10504 [doi]
PST - epublish
SO  - Bioeng Transl Med. 2023 Mar 21;8(3):e10504. doi: 10.1002/btm2.10504. eCollection 
      2023 May.