PMID- 37210473
OWN - NLM
STAT- MEDLINE
DCOM- 20230623
LR  - 20230701
IS  - 1525-3163 (Electronic)
IS  - 0021-8812 (Print)
IS  - 0021-8812 (Linking)
VI  - 101
DP  - 2023 Jan 3
TI  - Host-genetic-based outcome of co-infection by PCV2b and PRRSV in pigs.
LID - 10.1093/jas/skad164 [doi]
LID - skad164
AB  - Replication of porcine circovirus type 2 (PCV2), an important worldwide swine 
      pathogen, has been demonstrated to be influenced by host genotype. Specifically, 
      a missense DNA polymorphism (SYNGR2 p.Arg63Cys) within the SYNGR2 gene was 
      demonstrated to contribute to variation in PCV2b viral load and subsequent immune 
      response following infection. PCV2 is known to induce immunosuppression leading 
      to an increase in susceptibility to subsequent infections with other viral 
      pathogens such as porcine reproductive and respiratory syndrome virus (PRRSV). In 
      order to assess the role of SYNGR2 p.Arg63Cys in co-infections, pigs homozygous 
      for the favorable SYNGR2 p.63Cys (N = 30) and unfavorable SYNGR2 p.63Arg (N = 29) 
      alleles were infected with PCV2b followed a week later by a challenge with PRRSV. 
      A lower PCV2b viremia (P < 0.001) and PCV2-specific IgM antibodies (P < 0.005) 
      were observed in SYNGR2 p.63Cys compared to SYNGR2 p.63Arg genotypes. No 
      significant differences in PRRSV viremia and specific IgG antibodies were 
      observed between SYNGR2 genotypes. Lung histology score, an indicator of disease 
      severity, was lower in the pigs with SYNGR2 p.63Cys genotypes (P < 0.05). 
      Variation in the lung histology scores within SYNGR2 genotypes suggests that 
      additional factors, environmental and/or genetic, could be involved in disease 
      severity.
CI  - (c) The Author(s) 2023. Published by Oxford University Press on behalf of the 
      American Society of Animal Science. All rights reserved. For permissions, please 
      e-mail: journals.permissions@oup.com.
FAU - Eaton, Christian W
AU  - Eaton CW
AD  - Animal Science Department, University of Nebraska-Lincoln, Lincoln, NE 68583, 
      USA.
AD  - School of Veterinary Medicine and Biomedical Sciences, University of 
      Nebraska-Lincoln, Lincoln, NE 68583, USA.
FAU - Vu, Hiep L
AU  - Vu HL
AD  - Animal Science Department, University of Nebraska-Lincoln, Lincoln, NE 68583, 
      USA.
FAU - Hodges, Arabella L
AU  - Hodges AL
AD  - Animal Science Department, University of Nebraska-Lincoln, Lincoln, NE 68583, 
      USA.
FAU - Harris, Seth P
AU  - Harris SP
AD  - Veterinary Diagnostic Center, School of Veterinary Medicine and Biomedical 
      Sciences, University of Nebraska-Lincoln, Lincoln, NE 68583, USA.
FAU - Kachman, Stephen D
AU  - Kachman SD
AD  - Department of Statistics, University of Nebraska-Lincoln, Lincoln, NE 68583, USA.
FAU - Ciobanu, Daniel C
AU  - Ciobanu DC
AUID- ORCID: 0000-0002-7301-1339
AD  - Animal Science Department, University of Nebraska-Lincoln, Lincoln, NE 68583, 
      USA.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Anim Sci
JT  - Journal of animal science
JID - 8003002
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Swine
MH  - Animals
MH  - *Porcine respiratory and reproductive syndrome virus/genetics
MH  - *Swine Diseases/pathology
MH  - Viremia/veterinary
MH  - *Coinfection/veterinary
MH  - Antibodies, Viral
MH  - *Circoviridae Infections/veterinary/pathology
MH  - *Circovirus/genetics
MH  - *Porcine Reproductive and Respiratory Syndrome
PMC - PMC10284038
OAB - Porcine circovirus type 2 (PCV2) is an important virus involved in the onset of a 
      group of severe disease symptoms commonly known as porcine circovirus associated 
      diseases (PCVAD). Vaccination options exist for PCV2, though the severity of 
      PCVAD can be influenced by the presence of additional co-infecting pathogens, 
      such as porcine reproductive and respiratory syndrome virus (PRRSV), for which 
      vaccination is still a challenge. Host genetic resistance is a potential avenue 
      for solving this problem. Previously, a genetic polymorphism in the SYNGR2 gene 
      was found to be associated with PCV2b viremia and immune response. The aim of 
      this study was to determine the impact of this polymorphism in pigs 
      experimentally co-infected with PCV2b and PRRSV. Pigs were weighed, and blood was 
      collected at various days following infection to measure viremia and antibodies. 
      Histological analysis was performed at the experiment completion to assess 
      disease severity in lungs and lymph nodes. The results showed that variation 
      within the SYNGR2 gene is involved in PCV2b disease progression including lung 
      histology scores, but no evidence was seen in response to PRRSV infection.
OABL- eng
OTO - NOTNLM
OT  - PCV2
OT  - PRRSV
OT  - SYNGR2
OT  - host-genotype
OT  - pig
OT  - virus
COIS- The authors declare no real or perceived conflicts of interest.
EDAT- 2023/05/21 01:05
MHDA- 2023/06/23 06:42
PMCR- 2024/05/20
CRDT- 2023/05/20 23:09
PHST- 2022/12/21 00:00 [received]
PHST- 2023/05/18 00:00 [accepted]
PHST- 2024/05/20 00:00 [pmc-release]
PHST- 2023/06/23 06:42 [medline]
PHST- 2023/05/21 01:05 [pubmed]
PHST- 2023/05/20 23:09 [entrez]
AID - 7175049 [pii]
AID - skad164 [pii]
AID - 10.1093/jas/skad164 [doi]
PST - ppublish
SO  - J Anim Sci. 2023 Jan 3;101:skad164. doi: 10.1093/jas/skad164.