PMID- 37213515
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230523
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 14
DP  - 2023
TI  - Expanding the taxonomic and environmental extent of an underexplored carbon 
      metabolism-oxalotrophy.
PG  - 1161937
LID - 10.3389/fmicb.2023.1161937 [doi]
LID - 1161937
AB  - Oxalate serves various functions in the biological processes of plants, fungi, 
      bacteria, and animals. It occurs naturally in the minerals weddellite and 
      whewellite (calcium oxalates) or as oxalic acid. The environmental accumulation 
      of oxalate is disproportionately low compared to the prevalence of highly 
      productive oxalogens, namely plants. It is hypothesized that oxalotrophic 
      microbes limit oxalate accumulation by degrading oxalate minerals to carbonates 
      via an under-explored biogeochemical cycle known as the oxalate-carbonate pathway 
      (OCP). Neither the diversity nor the ecology of oxalotrophic bacteria is fully 
      understood. This research investigated the phylogenetic relationships of the 
      bacterial genes oxc, frc, oxdC, and oxlT, which encode key enzymes for 
      oxalotrophy, using bioinformatic approaches and publicly available omics 
      datasets. Phylogenetic trees of oxc and oxdC genes demonstrated grouping by both 
      source environment and taxonomy. All four trees included genes from 
      metagenome-assembled genomes (MAGs) that contained novel lineages and 
      environments for oxalotrophs. In particular, sequences of each gene were 
      recovered from marine environments. These results were supported with marine 
      transcriptome sequences and description of key amino acid residue conservation. 
      Additionally, we investigated the theoretical energy yield from oxalotrophy 
      across marine-relevant pressure and temperature conditions and found similar 
      standard state Gibbs free energy to "low energy" marine sediment metabolisms, 
      such as anaerobic oxidation of methane coupled to sulfate reduction. These 
      findings suggest further need to understand the role of bacterial oxalotrophy in 
      the OCP, particularly in marine environments, and its contribution to global 
      carbon cycling.
CI  - Copyright (c) 2023 Sonke and Trembath-Reichert.
FAU - Sonke, Alexander
AU  - Sonke A
AD  - School of Earth and Space Exploration, Arizona State University, Tempe, AZ, 
      United States.
FAU - Trembath-Reichert, Elizabeth
AU  - Trembath-Reichert E
AD  - School of Earth and Space Exploration, Arizona State University, Tempe, AZ, 
      United States.
LA  - eng
PT  - Journal Article
DEP - 20230504
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC10192776
OTO - NOTNLM
OT  - biosignature
OT  - carbon cycle
OT  - carbon sequestration
OT  - oxalate
OT  - oxalate-carbonate pathway
OT  - oxalotrophy
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/05/22 13:04
MHDA- 2023/05/22 13:05
PMCR- 2023/05/04
CRDT- 2023/05/22 12:00
PHST- 2023/02/08 00:00 [received]
PHST- 2023/04/11 00:00 [accepted]
PHST- 2023/05/22 13:05 [medline]
PHST- 2023/05/22 13:04 [pubmed]
PHST- 2023/05/22 12:00 [entrez]
PHST- 2023/05/04 00:00 [pmc-release]
AID - 10.3389/fmicb.2023.1161937 [doi]
PST - epublish
SO  - Front Microbiol. 2023 May 4;14:1161937. doi: 10.3389/fmicb.2023.1161937. 
      eCollection 2023.