PMID- 3721401
OWN - NLM
STAT- MEDLINE
DCOM- 19860731
LR  - 20061115
IS  - 0367-6102 (Print)
IS  - 0367-6102 (Linking)
VI  - 61
IP  - 2
DP  - 1986 Mar
TI  - [Development of radioimmunoassay for plasma arginine vasopressin and its 
      application to the diagnosis of children with central diabetes insipidus].
PG  - 303-13
AB  - For measurement of plasma arginine vasopressin (AVP) in children with central 
      diabetes insipidus (DI), a sensitive and specific radioimmunoassay (RIA) for AVP 
      was developed. The anti AVP serum used in this assay bound 50 percent of an 
      purified 125-I-labeled-AVP (125I-AVP) at a final dilution of 5 X 10(4) and its 
      cross reaction with lysine vasopressin, 1-deamino-8-D-arginine vasopressin and 
      oxytocin were 7, 15, and 0%, respectively. Column chromatography of Sephadex G-25 
      superfine was used for purification of 125I-AVP and four major peaks of 
      radioactivity were observed. The purified 125I-AVP was found in 3rd peak by the 
      highest binding activity with the anti-AVP serum. On the other hand, extraction 
      of AVP from plasma was carried out by the acetone extraction procedure and as 
      little as 1.2 pg per milliliter of AVP in plasma was detected in this RIA. In 
      dehydration state plasma concentration of AVP was 4.5 +/- 1.1 pg/ml (mean +/- SE) 
      in normal seven children (control), 1.5 +/- 0.2 pg/ml in six patients with 
      complete DI and 3.4 +/- 0.6 pg/ml in five patients with partial DI diagnosed with 
      Miller test (complete DI vs partial DI; p less than 0.02, partial DI vs control; 
      no significant). The evaluation of diagnostic value of AVP level measured with 
      RIA in complete DI and partial DI diagnosed with Miller test based on urine 
      osmolality was discussed.
FAU - Kato, T
AU  - Kato T
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PL  - Japan
TA  - Hokkaido Igaku Zasshi
JT  - [Hokkaido igaku zasshi] The Hokkaido journal of medical science
JID - 17410290R
RN  - 113-79-1 (Arginine Vasopressin)
SB  - IM
MH  - Adolescent
MH  - Arginine Vasopressin/*blood
MH  - Child
MH  - Child, Preschool
MH  - Diabetes Insipidus/*diagnosis
MH  - Female
MH  - Humans
MH  - Male
MH  - Osmotic Pressure
MH  - Radioimmunoassay/*methods
EDAT- 1986/03/01 00:00
MHDA- 1986/03/01 00:01
CRDT- 1986/03/01 00:00
PHST- 1986/03/01 00:00 [pubmed]
PHST- 1986/03/01 00:01 [medline]
PHST- 1986/03/01 00:00 [entrez]
PST - ppublish
SO  - Hokkaido Igaku Zasshi. 1986 Mar;61(2):303-13.