PMID- 37215982
OWN - NLM
STAT- MEDLINE
DCOM- 20230530
LR  - 20230710
IS  - 1449-2288 (Electronic)
IS  - 1449-2288 (Linking)
VI  - 19
IP  - 8
DP  - 2023
TI  - Increased methylation of ZNF671 suppresses tumor progression by promoting MAPK6 
      transcription in laryngeal carcinoma.
PG  - 2443-2457
LID - 10.7150/ijbs.82692 [doi]
AB  - Background: Laryngeal squamous cell carcinoma (LSCC) is a malignant tumor of the 
      head and neck, the exact mechanism of which has not been explored. Methods: By 
      analyzing the GEO data, we found the highly methylated and low expression gene 
      ZNF671. The expression level of ZNF671 in clinical samples was verified by 
      RT-PCR, western blotting and methylation-specific PCR. The function of ZNF671 in 
      LSCC was detected by cell culture and transfection, MTT, Edu, TUNEL assays and 
      flow cytometry analysis. The binding sites of ZNF671 to MAPK6 promoter region 
      were detected and verified by luciferase reporter gene and chromatin 
      immunoprecipitation. Finally, the effect of ZNF671 on LSCC tumors was tested in 
      vivo. Results: In this study, by analyzing GEO data GSE178218 and GSE59102, we 
      found that zinc finger protein (ZNF671) expression was decreased, and DNA 
      methylation level was increased in laryngeal cancer. Moreover, the abnormal 
      expression of ZNF671 was associated with poor survival prognosis of patients. In 
      addition, we found that overexpression of ZNF671 could inhibit the viability, 
      proliferation, migration and invasion of LSCC cells, while promoting cell 
      apoptosis. In contrast, the opposite effects were observed after knockdown of 
      ZNF671. Through the prediction website and chromatin immunoprecipitation and 
      luciferase reporter experiments, it was found that ZNF671 could bind to the 
      promoter region of MAPK6, thereby inhibiting the expression of MPAK6. In vivo 
      experiments confirmed that overexpression of ZNF671 could inhibit tumor growth. 
      Conclusion: Our study found that ZNF671 expression was down-regulated in LSCC. 
      ZNF671 up-regulates the expression of MAPK6 by binding to its promoter region, 
      thus participating in cell proliferation, migration and invasion in LSCC. Our 
      study may provide new ideas for early prediction and treatment of LSCC.
CI  - (c) The author(s).
FAU - Zhang, Wei
AU  - Zhang W
AD  - Department of Endocrinology, Shengjing Hospital of China Medical University, 
      Shenyang, Liaoning, 110004, China.
FAU - Liu, Ting
AU  - Liu T
AD  - Department of Otolaryngology Head and Neck Surgery, Shengjing Hospital of China 
      Medical University, Shenyang, Liaoning, 110004, China.
FAU - Li, Xinyi
AU  - Li X
AD  - Department of Otolaryngology Head and Neck Surgery, Shengjing Hospital of China 
      Medical University, Shenyang, Liaoning, 110004, China.
FAU - Li, Tianshu
AU  - Li T
AD  - Department of Otolaryngology Head and Neck Surgery, Shengjing Hospital of China 
      Medical University, Shenyang, Liaoning, 110004, China.
FAU - Ma, Xiangchi
AU  - Ma X
AD  - Department of Otolaryngology Head and Neck Surgery, Shengjing Hospital of China 
      Medical University, Shenyang, Liaoning, 110004, China.
FAU - Zhao, Dongxu
AU  - Zhao D
AD  - Department of Otolaryngology Head and Neck Surgery, Shengjing Hospital of China 
      Medical University, Shenyang, Liaoning, 110004, China.
FAU - Zhao, Xudong
AU  - Zhao X
AD  - Department of Otolaryngology Head and Neck Surgery, Shengjing Hospital of China 
      Medical University, Shenyang, Liaoning, 110004, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230508
PL  - Australia
TA  - Int J Biol Sci
JT  - International journal of biological sciences
JID - 101235568
RN  - 0 (MicroRNAs)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 0 (ZNF671 protein, human)
SB  - IM
MH  - Humans
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - DNA Methylation/genetics
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - *Laryngeal Neoplasms/genetics/pathology
MH  - *MicroRNAs/metabolism
MH  - *Squamous Cell Carcinoma of Head and Neck/genetics/pathology
MH  - *Tumor Suppressor Proteins/genetics
PMC - PMC10197888
OTO - NOTNLM
OT  - Laryngeal carcinoma
OT  - MAPK6
OT  - ZNF671
OT  - methylation
OT  - proliferation
COIS- Competing Interests: The authors have declared that no competing interest exists.
EDAT- 2023/05/22 19:11
MHDA- 2023/05/24 06:42
PMCR- 2023/01/01
CRDT- 2023/05/22 12:32
PHST- 2023/01/16 00:00 [received]
PHST- 2023/04/15 00:00 [accepted]
PHST- 2023/05/24 06:42 [medline]
PHST- 2023/05/22 19:11 [pubmed]
PHST- 2023/05/22 12:32 [entrez]
PHST- 2023/01/01 00:00 [pmc-release]
AID - ijbsv19p2443 [pii]
AID - 10.7150/ijbs.82692 [doi]
PST - epublish
SO  - Int J Biol Sci. 2023 May 8;19(8):2443-2457. doi: 10.7150/ijbs.82692. eCollection 
      2023.