PMID- 37217041
OWN - NLM
STAT- MEDLINE
DCOM- 20230619
LR  - 20230619
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 242
IP  - Pt 3
DP  - 2023 Jul 1
TI  - Comprehensive analysis of OpHD-ZIP transcription factors related to the 
      regulation of camptothecin biosynthesis in Ophiorrhiza pumila.
PG  - 124910
LID - S0141-8130(23)01804-4 [pii]
LID - 10.1016/j.ijbiomac.2023.124910 [doi]
AB  - Ophiorrhiza pumila, as a folk herb belonging to the Rubiaceae family, has become 
      a potential source of camptothecin (CPT), which is a monoterpenoid indole 
      alkaloid with good antitumor property. However, the camptothecin content in this 
      herb is low, and is far from meeting the increasing clinical demand. 
      Understanding the transcriptional regulation of camptothecin biosynthesis 
      provides an effective strategy for improvement of camptothecin yield. Previous 
      studies have demonstrated several transcription factors that are related to 
      camptothecin biosynthesis, while the functions of HD-ZIP members in O. pumila 
      have not been investigated yet. In this study, 32 OpHD-ZIP transcription factor 
      members were genome-wide identified. Phylogenetic tree showed that these OpHD-ZIP 
      proteins are divided into four subfamilies. Based on the transcriptome data, nine 
      OpHD-ZIP genes were shown to be predominantly expressed in O. pumila roots, which 
      were in line with the camptothecin biosynthetic genes. Co-expression analysis 
      showed that OpHD-ZIP7 and OpHD-ZIP20 were potentially related to the modulation 
      of camptothecin biosynthesis. Dual-luciferase reporter assays (Dual-LUC) showed 
      that both OpHD-ZIP7 and OpHD-ZIP20 could activate the expression of camptothecin 
      biosynthetic genes OpIO and OpTDC. In conclusion, this study offered the 
      promising data for exploring the roles of OpHD-ZIP transcription factors in 
      regulating camptothecin biosynthesis.
CI  - Copyright (c) 2023 Elsevier B.V. All rights reserved.
FAU - Wang, Jingyi
AU  - Wang J
AD  - Zhejiang International Science and Technology Cooperation Base for Active 
      Ingredients of Medicinal and Edible Plants and Health, Jinhua Academy, School of 
      Pharmaceutical Sciences, Academy of Chinese Medical Science, Zhejiang Chinese 
      Medical University, Hangzhou 310053, China.
FAU - Li, Yongpeng
AU  - Li Y
AD  - Zhejiang International Science and Technology Cooperation Base for Active 
      Ingredients of Medicinal and Edible Plants and Health, Jinhua Academy, School of 
      Pharmaceutical Sciences, Academy of Chinese Medical Science, Zhejiang Chinese 
      Medical University, Hangzhou 310053, China.
FAU - Yang, Yinkai
AU  - Yang Y
AD  - Zhejiang International Science and Technology Cooperation Base for Active 
      Ingredients of Medicinal and Edible Plants and Health, Jinhua Academy, School of 
      Pharmaceutical Sciences, Academy of Chinese Medical Science, Zhejiang Chinese 
      Medical University, Hangzhou 310053, China.
FAU - Xiao, Chengyu
AU  - Xiao C
AD  - Zhejiang International Science and Technology Cooperation Base for Active 
      Ingredients of Medicinal and Edible Plants and Health, Jinhua Academy, School of 
      Pharmaceutical Sciences, Academy of Chinese Medical Science, Zhejiang Chinese 
      Medical University, Hangzhou 310053, China.
FAU - Ruan, Qingyan
AU  - Ruan Q
AD  - Zhejiang International Science and Technology Cooperation Base for Active 
      Ingredients of Medicinal and Edible Plants and Health, Jinhua Academy, School of 
      Pharmaceutical Sciences, Academy of Chinese Medical Science, Zhejiang Chinese 
      Medical University, Hangzhou 310053, China.
FAU - Li, Pengyang
AU  - Li P
AD  - Zhejiang International Science and Technology Cooperation Base for Active 
      Ingredients of Medicinal and Edible Plants and Health, Jinhua Academy, School of 
      Pharmaceutical Sciences, Academy of Chinese Medical Science, Zhejiang Chinese 
      Medical University, Hangzhou 310053, China.
FAU - Zhou, Qin
AU  - Zhou Q
AD  - Zhejiang International Science and Technology Cooperation Base for Active 
      Ingredients of Medicinal and Edible Plants and Health, Jinhua Academy, School of 
      Pharmaceutical Sciences, Academy of Chinese Medical Science, Zhejiang Chinese 
      Medical University, Hangzhou 310053, China.
FAU - Sheng, Miaomiao
AU  - Sheng M
AD  - Zhejiang International Science and Technology Cooperation Base for Active 
      Ingredients of Medicinal and Edible Plants and Health, Jinhua Academy, School of 
      Pharmaceutical Sciences, Academy of Chinese Medical Science, Zhejiang Chinese 
      Medical University, Hangzhou 310053, China.
FAU - Hao, Xiaolong
AU  - Hao X
AD  - Zhejiang International Science and Technology Cooperation Base for Active 
      Ingredients of Medicinal and Edible Plants and Health, Jinhua Academy, School of 
      Pharmaceutical Sciences, Academy of Chinese Medical Science, Zhejiang Chinese 
      Medical University, Hangzhou 310053, China. Electronic address: 
      haoxiaolong@zcmu.edu.cn.
FAU - Kai, Guoyin
AU  - Kai G
AD  - Zhejiang International Science and Technology Cooperation Base for Active 
      Ingredients of Medicinal and Edible Plants and Health, Jinhua Academy, School of 
      Pharmaceutical Sciences, Academy of Chinese Medical Science, Zhejiang Chinese 
      Medical University, Hangzhou 310053, China. Electronic address: 
      guoyinkai1@126.com.
LA  - eng
PT  - Journal Article
DEP - 20230520
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - XT3Z54Z28A (Camptothecin)
RN  - 0 (Transcription Factors)
RN  - 0 (Cation Transport Proteins)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
MH  - Camptothecin
MH  - Transcription Factors/genetics/metabolism
MH  - Phylogeny
MH  - *Cation Transport Proteins/genetics
MH  - Endoplasmic Reticulum/metabolism
MH  - Zinc/metabolism
MH  - *Rubiaceae/genetics
OTO - NOTNLM
OT  - Genome-wide identification
OT  - HD-ZIP transcription factor
OT  - Ophiorrhiza pumila
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/05/23 01:06
MHDA- 2023/06/19 13:08
CRDT- 2023/05/22 19:19
PHST- 2023/03/31 00:00 [received]
PHST- 2023/05/12 00:00 [revised]
PHST- 2023/05/13 00:00 [accepted]
PHST- 2023/06/19 13:08 [medline]
PHST- 2023/05/23 01:06 [pubmed]
PHST- 2023/05/22 19:19 [entrez]
AID - S0141-8130(23)01804-4 [pii]
AID - 10.1016/j.ijbiomac.2023.124910 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2023 Jul 1;242(Pt 3):124910. doi: 
      10.1016/j.ijbiomac.2023.124910. Epub 2023 May 20.