PMID- 37219914
OWN - NLM
STAT- MEDLINE
DCOM- 20230713
LR  - 20230718
IS  - 1557-7430 (Electronic)
IS  - 1044-5498 (Linking)
VI  - 42
IP  - 7
DP  - 2023 Jul
TI  - The Novel Circular RNA circTRIO Silence Inhibits the Progression of Laryngeal 
      Squamous Cell Carcinoma.
PG  - 421-432
LID - 10.1089/dna.2023.0043 [doi]
AB  - Laryngeal squamous cell carcinoma (LSCC) is the most common and prevalent 
      malignant tumor in head and neck squamous cell carcinoma. Recent studies have 
      shown that circular RNAs (circRNAs) play a vital role in cancer development, but 
      their specific role in the tumorigenesis and development of LSCC remains unclear. 
      We selected five pairs of LSCC tumor tissues and paracancerous tissues for RNA 
      sequencing. Reverse transcription-quantitative PCR (RT-qPCR), Sanger sequencing, 
      and fluorescence in situ hybridization were utilized to study the expression, 
      localization, and clinical significance of circTRIO in LSCC tissues, and TU212 
      and TU686 cell lines. Furthermore, cell counting Kit-8, colony-forming assay, 
      Transwell, and flow cytometry assays were assessed to illustrate the crucial role 
      played by the circTRIO in proliferation, colony-forming ability, migration, and 
      apoptosis in LSCC cells. Finally, the molecule's role as a microRNA (miRNA) 
      sponge was analyzed. In the results, we screened out a promising upregulated 
      novel circRNA-circTRIO in LSCC tumor tissues compared with paracancerous tissues 
      using RNA sequencing. Then, we used qPCR to evaluate the expression of circTRIO 
      in 20 additional paired LSCC tissues and two cells, and the results showed that 
      circTRIO was highly expressed in LSCC and that this high expression was closely 
      related to the malignant progression of LSCC. Furthermore, we examined circTRIO 
      expression in the Gene Expression Omnibus data sets GSE142083 and GSE27020, and 
      circTRIO expression was considerably higher in tumor tissues compared with the 
      adjacent tissues. Kaplan-Meier survival analysis showed that the expression of 
      circTRIO was associated with worse disease-free survival. The Gene Set Enrichment 
      Analysis biological pathway evaluation results demonstrated that circTRIO was 
      mainly enriched in cancer pathways. Moreover, we confirmed that silencing 
      circTRIOs can help to significantly inhibit LSCC cell proliferation and migration 
      while triggering apoptosis. Upregulated circTRIO expression levels may play an 
      important role in the tumorigenesis and development of LSCC.
FAU - Wang, Jing
AU  - Wang J
AD  - Department of Pathology, Beijing Tongren Hospital, Capital Medical University, 
      Beijing, China.
AD  - Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology, Beijing, 
      China.
FAU - He, Shujin
AU  - He S
AD  - Department of Pathology, Beijing Tongren Hospital, Capital Medical University, 
      Beijing, China.
AD  - Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology, Beijing, 
      China.
FAU - Wang, Dingding
AU  - Wang D
AD  - Department of Neonatal Surgery, Beijing Children's Hospital, Capital Medical 
      University, Beijing, China.
FAU - Jing, Yanlei
AU  - Jing Y
AD  - Department of Pathology, Beijing Tongren Hospital, Capital Medical University, 
      Beijing, China.
AD  - Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology, Beijing, 
      China.
FAU - Shen, Wenping
AU  - Shen W
AD  - Department of Pathology, Beijing Tongren Hospital, Capital Medical University, 
      Beijing, China.
AD  - Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology, Beijing, 
      China.
FAU - Huang, Anqi
AU  - Huang A
AD  - Department of Pathology, Beijing Tongren Hospital, Capital Medical University, 
      Beijing, China.
AD  - Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology, Beijing, 
      China.
FAU - Zhou, Zhe
AU  - Zhou Z
AD  - Department of Pathology, Beijing Tongren Hospital, Capital Medical University, 
      Beijing, China.
AD  - Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology, Beijing, 
      China.
FAU - Liu, Honggang
AU  - Liu H
AD  - Department of Pathology, Beijing Tongren Hospital, Capital Medical University, 
      Beijing, China.
AD  - Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology, Beijing, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20230522
PL  - United States
TA  - DNA Cell Biol
JT  - DNA and cell biology
JID - 9004522
RN  - 0 (RNA, Circular)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Humans
MH  - Squamous Cell Carcinoma of Head and Neck/genetics
MH  - RNA, Circular/genetics
MH  - *Carcinoma, Squamous Cell/pathology
MH  - *Laryngeal Neoplasms/genetics/pathology
MH  - In Situ Hybridization, Fluorescence
MH  - *MicroRNAs/genetics/metabolism
MH  - *Head and Neck Neoplasms/genetics
MH  - Carcinogenesis/genetics
MH  - Cell Proliferation/genetics
MH  - Cell Line, Tumor
MH  - Gene Expression Regulation, Neoplastic/genetics
OTO - NOTNLM
OT  - circTRIO
OT  - circular RNA
OT  - laryngeal squamous cell carcinoma
EDAT- 2023/05/23 13:05
MHDA- 2023/07/13 06:42
CRDT- 2023/05/23 11:34
PHST- 2023/07/13 06:42 [medline]
PHST- 2023/05/23 13:05 [pubmed]
PHST- 2023/05/23 11:34 [entrez]
AID - 10.1089/dna.2023.0043 [doi]
PST - ppublish
SO  - DNA Cell Biol. 2023 Jul;42(7):421-432. doi: 10.1089/dna.2023.0043. Epub 2023 May 
      22.