PMID- 37220694
OWN - NLM
STAT- MEDLINE
DCOM- 20230619
LR  - 20230619
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 120
DP  - 2023 Jul
TI  - Oncostatin M regulates macrophages polarization in osseointegration via 
      yes-associated protein.
PG  - 110348
LID - S1567-5769(23)00671-9 [pii]
LID - 10.1016/j.intimp.2023.110348 [doi]
AB  - OBJECTIVES: Oncostatin M(OSM), secreted by monocytes and macrophages, has been 
      noted to participate in bone homeostasis and macrophage polarization, which might 
      be regulated by yes-associated protein (YAP). This study aimed to elucidate the 
      influence and mechanisms of OSM-YAP on macrophages polarization in 
      osseointegration. MATERIAL AND METHODS: In vitro, flow cytometry, real-time PCR, 
      and Elisa were performed to evaluate inflammatory function in bone marrow-derived 
      macrophages (BMDMs) with OSM, siOSMR, and YAP inhibitor verteporfin (VP). In 
      vivo, macrophage-specific YAP-deficient mice were generated to investigate the 
      role of OSM via YAP signaling in osseointegration. RESULTS: This study 
      demonstrated that OSM could inhibit the M1 polarization, promote the M2 
      polarization, and induce the expression of osteogenic-related factors via VP. The 
      conditional knock-out of YAP inhibited the osseointegration in mice, and promoted 
      the inflammatory reaction around the implants, while OSM could restore the 
      effect. CONCLUSIONS: Our results demonstrated that OSM might play an important 
      role in the polarization of BMDMs, and bone formation around dental and femoral 
      implants. This effect was closely conducted by Hippo-YAP pathway. CLINICAL 
      SIGNIFICANCE: Understanding the role and mechanism of OSM in macrophage 
      polarization around dental implants could improve comprehension of signal network 
      of osseointegration, and it might offer a potential target of therapies to 
      accelerate osseointegration and reduce inflammatory reactions.
CI  - Copyright (c) 2023 Elsevier B.V. All rights reserved.
FAU - Yuan, Ying
AU  - Yuan Y
AD  - State Key Laboratory of Oral Diseases & National Clinical Research Center for 
      Oral Diseases & Department of Oral Implantology, West China Hospital of 
      Stomatology, Sichuan University, Chengdu, China; Stomatological Hospital of 
      Chongqing Medical University, Chongqing Key Laboratory of Oral Diseases and 
      Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical 
      Engineering of Higher Education, Chongqing 400016, China.
FAU - Zhang, Qin
AU  - Zhang Q
AD  - State Key Laboratory of Oral Diseases & National Clinical Research Center for 
      Oral Diseases & Department of Oral Implantology, West China Hospital of 
      Stomatology, Sichuan University, Chengdu, China.
FAU - Wu, Bingfeng
AU  - Wu B
AD  - State Key Laboratory of Oral Diseases & National Clinical Research Center for 
      Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      China.
FAU - Huang, Tianyu
AU  - Huang T
AD  - State Key Laboratory of Oral Diseases & National Clinical Research Center for 
      Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      China.
FAU - Gong, Ping
AU  - Gong P
AD  - State Key Laboratory of Oral Diseases & National Clinical Research Center for 
      Oral Diseases & Department of Oral Implantology, West China Hospital of 
      Stomatology, Sichuan University, Chengdu, China.
FAU - Xiang, Lin
AU  - Xiang L
AD  - State Key Laboratory of Oral Diseases & National Clinical Research Center for 
      Oral Diseases & Department of Oral Implantology, West China Hospital of 
      Stomatology, Sichuan University, Chengdu, China. Electronic address: 
      dentistxiang@126.com.
LA  - eng
PT  - Journal Article
DEP - 20230521
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 106956-32-5 (Oncostatin M)
RN  - 0 (YAP-Signaling Proteins)
RN  - 0 (Yap1 protein, mouse)
RN  - 0 (Osm protein, mouse)
SB  - IM
MH  - Animals
MH  - Mice
MH  - Inflammation/metabolism
MH  - Macrophages
MH  - Oncostatin M/metabolism
MH  - *Osseointegration
MH  - Signal Transduction
MH  - *YAP-Signaling Proteins
OTO - NOTNLM
OT  - Bone biology
OT  - Bone remodeling/regeneration
OT  - Cell signaling
OT  - Dental implant(s)
OT  - Immunity
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/05/23 19:09
MHDA- 2023/06/16 06:42
CRDT- 2023/05/23 18:00
PHST- 2023/03/06 00:00 [received]
PHST- 2023/05/04 00:00 [revised]
PHST- 2023/05/12 00:00 [accepted]
PHST- 2023/06/16 06:42 [medline]
PHST- 2023/05/23 19:09 [pubmed]
PHST- 2023/05/23 18:00 [entrez]
AID - S1567-5769(23)00671-9 [pii]
AID - 10.1016/j.intimp.2023.110348 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2023 Jul;120:110348. doi: 10.1016/j.intimp.2023.110348. Epub 
      2023 May 21.