PMID- 37222145
OWN - NLM
STAT- MEDLINE
DCOM- 20230608
LR  - 20230617
IS  - 2050-7518 (Electronic)
IS  - 2050-750X (Linking)
VI  - 11
IP  - 22
DP  - 2023 Jun 7
TI  - A pDNA/rapamycin nanocomposite coating on interventional balloons for inhibiting 
      neointimal hyperplasia.
PG  - 4882-4889
LID - 10.1039/d3tb00344b [doi]
AB  - Drug-coated balloon (DCB) is a therapeutic method that can effectively deliver 
      antiproliferative drugs such as paclitaxel and rapamycin (RAPA) with no permanent 
      implants left behind. However, delayed reendothelialization due to the toxicity 
      of the delivered drugs leads to poor therapeutic effects. Here, we propose a new 
      design of DCB coating, which incorporates both vascular endothelial growth factor 
      (VEGF)-encoding plasmid DNA (pDNA) that can promote endothelial repair and RAPA 
      into protamine sulfate (PrS). We demonstrate that the PrS/pDNA/RAPA coating had 
      stability and good anticoagulation properties in vitro. We further show that the 
      coating exhibited excellent transfer capacity from balloon substrates to vessel 
      walls both in vitro and in vivo. Furthermore, the PrS/pDNA/RAPA coating 
      effectively inhibited neointimal hyperplasia after balloon-induced vascular 
      injuries through the down-regulation of the mammalian target of Rapamycin (mTOR) 
      and promoted endothelium regeneration through increased expression of VEGF in 
      vivo. These data indicate that our nanocomposite coating has great potential for 
      use as a novel coating of DCB to treat neointimal hyperplasia after vascular 
      injuries.
FAU - Lai, Yu-Xian
AU  - Lai YX
AD  - MOE Key Laboratory of Macromolecular Synthesis and Functionalization, 
      International Research Center for X Polymers, Department of Polymer Science and 
      Engineering, Zhejiang University, Hangzhou, 310027, China. renkf@zju.edu.cn.
FAU - Fu, Jia-Yin
AU  - Fu JY
AD  - Key Laboratory of Cardiovascular Intervention and Regenerative Medicine of 
      Zhejiang Province, Department of Cardiology, Sir Run Run Shaw Hospital, Zhejiang 
      University, Hangzhou, 310016, China. fugs@zju.edu.cn.
FAU - Wu, Shao-Fei
AU  - Wu SF
AD  - Key Laboratory of Cardiovascular Intervention and Regenerative Medicine of 
      Zhejiang Province, Department of Cardiology, Sir Run Run Shaw Hospital, Zhejiang 
      University, Hangzhou, 310016, China. fugs@zju.edu.cn.
FAU - Li, Ren-Yun
AU  - Li RY
AD  - MOE Key Laboratory of Macromolecular Synthesis and Functionalization, 
      International Research Center for X Polymers, Department of Polymer Science and 
      Engineering, Zhejiang University, Hangzhou, 310027, China. renkf@zju.edu.cn.
FAU - Hu, Jia-Qi
AU  - Hu JQ
AD  - MOE Key Laboratory of Macromolecular Synthesis and Functionalization, 
      International Research Center for X Polymers, Department of Polymer Science and 
      Engineering, Zhejiang University, Hangzhou, 310027, China. renkf@zju.edu.cn.
FAU - Wang, You-Xiang
AU  - Wang YX
AUID- ORCID: 0000-0002-7821-9470
AD  - MOE Key Laboratory of Macromolecular Synthesis and Functionalization, 
      International Research Center for X Polymers, Department of Polymer Science and 
      Engineering, Zhejiang University, Hangzhou, 310027, China. renkf@zju.edu.cn.
FAU - Martins, M Cristina L
AU  - Martins MCL
AUID- ORCID: 0000-0002-6574-4794
AD  - i3S-Instituto de Investigacao e Inovacao em Saude, Universidade do Porto, 
      P-4200-135, Porto, Portugal.
FAU - Ren, Ke-Feng
AU  - Ren KF
AUID- ORCID: 0000-0001-5456-984X
AD  - MOE Key Laboratory of Macromolecular Synthesis and Functionalization, 
      International Research Center for X Polymers, Department of Polymer Science and 
      Engineering, Zhejiang University, Hangzhou, 310027, China. renkf@zju.edu.cn.
AD  - Key Laboratory of Cardiovascular Intervention and Regenerative Medicine of 
      Zhejiang Province, Department of Cardiology, Sir Run Run Shaw Hospital, Zhejiang 
      University, Hangzhou, 310016, China. fugs@zju.edu.cn.
FAU - Ji, Jian
AU  - Ji J
AUID- ORCID: 0000-0001-9870-4038
AD  - MOE Key Laboratory of Macromolecular Synthesis and Functionalization, 
      International Research Center for X Polymers, Department of Polymer Science and 
      Engineering, Zhejiang University, Hangzhou, 310027, China. renkf@zju.edu.cn.
AD  - Key Laboratory of Cardiovascular Intervention and Regenerative Medicine of 
      Zhejiang Province, Department of Cardiology, Sir Run Run Shaw Hospital, Zhejiang 
      University, Hangzhou, 310016, China. fugs@zju.edu.cn.
FAU - Fu, Guo-Sheng
AU  - Fu GS
AD  - Key Laboratory of Cardiovascular Intervention and Regenerative Medicine of 
      Zhejiang Province, Department of Cardiology, Sir Run Run Shaw Hospital, Zhejiang 
      University, Hangzhou, 310016, China. fugs@zju.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230607
PL  - England
TA  - J Mater Chem B
JT  - Journal of materials chemistry. B
JID - 101598493
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - W36ZG6FT64 (Sirolimus)
RN  - 9007-49-2 (DNA)
RN  - 0 (Vascular Endothelial Growth Factors)
SB  - IM
MH  - Humans
MH  - *Vascular Endothelial Growth Factor A
MH  - Sirolimus/pharmacology
MH  - Hyperplasia/drug therapy
MH  - *Vascular System Injuries
MH  - Plasmids
MH  - DNA
MH  - Vascular Endothelial Growth Factors
EDAT- 2023/05/24 06:42
MHDA- 2023/06/08 06:42
CRDT- 2023/05/24 05:28
PHST- 2023/06/08 06:42 [medline]
PHST- 2023/05/24 06:42 [pubmed]
PHST- 2023/05/24 05:28 [entrez]
AID - 10.1039/d3tb00344b [doi]
PST - epublish
SO  - J Mater Chem B. 2023 Jun 7;11(22):4882-4889. doi: 10.1039/d3tb00344b.