PMID- 37224290
OWN - NLM
STAT- MEDLINE
DCOM- 20240223
LR  - 20240223
IS  - 1529-4242 (Electronic)
IS  - 0032-1052 (Linking)
VI  - 153
IP  - 3
DP  - 2024 Mar 1
TI  - A Calvarial Defect Model to Investigate the Osteogenic Potential of Umbilical 
      Cord Stem Cells in Bone Regeneration.
PG  - 637-646
LID - 10.1097/PRS.0000000000010754 [doi]
AB  - BACKGROUND: The standard graft material for alveolar cleft repair (ACR) is 
      autogenous iliac crest. A promising alternative potential graft adjunct-newborn 
      human umbilical cord mesenchymal stem cells (h-UCMSCs)-has yet to be explored in 
      vivo. Their capacity for self-renewal, multipotent differentiation, and 
      proliferation allows h-UCMSCs to be harnessed for regenerative medicine. This 
      study sought to evaluate the efficacy of using tissue-derived h-UCMSCs and their 
      osteogenic capabilities to improve ACR in a murine model. METHODS: Foxn1 mice 
      were separated into three groups with the following calvarial defects: no 
      treatment (empty defect; n = 6), poly(D,L-lactide-co-glycolide) (PLGA) scaffold ( 
      n = 6), or h-UCMSCs with PLGA ( n = 4). Bilateral 2-mm-diameter parietal bone 
      critical-sized defects were created using a dental drill. Microcomputed 
      tomography (microCT) imaging was performed 1, 2, 3, and 4 weeks postoperatively. 
      The mice were euthanized 4 weeks postoperatively for RNAScope, 
      immunohistochemical, and histological analysis. RESULTS: No mice experienced 
      complications during the follow-up period. MicroCT imaging and histological 
      analysis demonstrated that the no-treatment and PLGA-only defects remained patent 
      without significant defect size differences across groups. In contrast, the 
      h-UCMSCs with PLGA group had significantly greater bone fill on microCT and 
      histological analysis. CONCLUSIONS: This study demonstrates a successful 
      calvarial defect model for the investigation of h-UCMSC-mediated osteogenesis and 
      bone repair. Evidence reveals that PLGA alone has neither short-term effects on 
      bone formation nor any unwanted side effects, making it an attractive scaffold. 
      Further investigation using h-UCMSCs with PLGA in larger animals is warranted to 
      advance future translation to patients requiring ACR. CLINICAL RELEVANCE 
      STATEMENT: The authors' results demonstrate a successful murine calvarial defect 
      model for the investigation of h-UCMSC-mediated osteogenesis and bone repair, and 
      they provide preliminary evidence for the safe and efficacious use of this graft 
      adjunct in alveolar cleft repair.
CI  - Copyright (c) 2023 by the American Society of Plastic Surgeons.
FAU - Stanton, Eloise
AU  - Stanton E
AD  - From the Center for Craniofacial Molecular Biology.
AD  - Keck School of Medicine.
AD  - Division of Plastic and Maxillofacial Surgery, Children's Hospital Los Angeles.
FAU - Feng, Jifan
AU  - Feng J
AD  - From the Center for Craniofacial Molecular Biology.
FAU - Kondra, Katelyn
AU  - Kondra K
AD  - Keck School of Medicine.
AD  - Division of Plastic and Maxillofacial Surgery, Children's Hospital Los Angeles.
FAU - Sanchez, Janet
AU  - Sanchez J
AD  - From the Center for Craniofacial Molecular Biology.
FAU - Jimenez, Christian
AU  - Jimenez C
AD  - Keck School of Medicine.
AD  - Division of Plastic and Maxillofacial Surgery, Children's Hospital Los Angeles.
FAU - Brown, Katherine S
AU  - Brown KS
AD  - Research and Development, CBR Systems, Inc., a CooperSurgical Company.
FAU - Skiles, Matthew L
AU  - Skiles ML
AD  - Research and Development, CBR Systems, Inc., a CooperSurgical Company.
FAU - Urata, Mark M
AU  - Urata MM
AD  - From the Center for Craniofacial Molecular Biology.
AD  - Keck School of Medicine.
AD  - Ostrow School of Dentistry, University of Southern California.
AD  - Division of Plastic and Maxillofacial Surgery, Children's Hospital Los Angeles.
FAU - Chai, Yang
AU  - Chai Y
AD  - From the Center for Craniofacial Molecular Biology.
AD  - Ostrow School of Dentistry, University of Southern California.
FAU - Hammoudeh, Jeffrey A
AU  - Hammoudeh JA
AD  - Keck School of Medicine.
AD  - Ostrow School of Dentistry, University of Southern California.
AD  - Division of Plastic and Maxillofacial Surgery, Children's Hospital Los Angeles.
LA  - eng
PT  - Journal Article
DEP - 20230524
PL  - United States
TA  - Plast Reconstr Surg
JT  - Plastic and reconstructive surgery
JID - 1306050
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
SB  - IM
MH  - Humans
MH  - Mice
MH  - Animals
MH  - *Osteogenesis
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - *Tissue Scaffolds
MH  - X-Ray Microtomography
MH  - Bone Regeneration
MH  - Stem Cells
MH  - Cell Differentiation
MH  - Umbilical Cord
MH  - Skull/surgery/pathology
EDAT- 2023/05/24 19:13
MHDA- 2024/02/23 06:43
CRDT- 2023/05/24 14:43
PHST- 2024/02/23 06:43 [medline]
PHST- 2023/05/24 19:13 [pubmed]
PHST- 2023/05/24 14:43 [entrez]
AID - 00006534-990000000-01918 [pii]
AID - 10.1097/PRS.0000000000010754 [doi]
PST - ppublish
SO  - Plast Reconstr Surg. 2024 Mar 1;153(3):637-646. doi: 
      10.1097/PRS.0000000000010754. Epub 2023 May 24.