PMID- 37225168 OWN - NLM STAT- MEDLINE DCOM- 20230623 LR - 20230623 IS - 1543-2742 (Electronic) IS - 1526-484X (Linking) VI - 33 IP - 4 DP - 2023 Jul 1 TI - Co-Ingestion of Branched-Chain Amino Acids and Carbohydrate Stimulates Myofibrillar Protein Synthesis Following Resistance Exercise in Trained Young Men. PG - 189-197 LID - 10.1123/ijsnem.2023-0015 [doi] AB - Branched-chain amino acids (BCAA) and carbohydrate (CHO) are commonly recommended postexercise supplements. However, no study has examined the interaction of CHO and BCAA ingestion on myofibrillar protein synthesis (MyoPS) rates following exercise. We aimed to determine the response of MyoPS to the co-ingestion of BCAA and CHO following an acute bout of resistance exercise. Ten resistance-trained young men completed two trials in counterbalanced order, ingesting isocaloric drinks containing either 30.6-g CHO plus 5.6-g BCAA (B + C) or 34.7-g CHO alone following a bout of unilateral, leg resistance exercise. MyoPS was measured postexercise with a primed, constant infusion of L-[ring13C6] phenylalanine and collection of muscle biopsies pre- and 4 hr postdrink ingestion. Blood samples were collected at time points before and after drink ingestion. Serum insulin concentrations increased to a similar extent in both trials (p > .05), peaking at 30 min postdrink ingestion. Plasma leucine (514 +/- 34 nmol/L), isoleucine (282 +/- 23 nmol/L), and valine (687 +/- 33 nmol/L) concentrations peaked at 0.5 hr postdrink in B + C and remained elevated for 3 hr during exercise recovery. MyoPS was approximately 15% greater (95% confidence interval [-0.002, 0.028], p = .039, Cohen's d = 0.63) in B + C (0.128%/hr +/- 0.011%/hr) than CHO alone (0.115%/hr +/- 0.011%/hr) over the 4 hr postexercise period. Co-ingestion of BCAA and CHO augments the acute response of MyoPS to resistance exercise in trained young males. FAU - Jackman, Sarah R AU - Jackman SR AD - Public Health and Sport Sciences, University of Exeter Medical School, Faculty of Health and Life Sciences, University of Exeter, Exeter,United Kingdom. FAU - Wallis, Gareth A AU - Wallis GA AD - School of Sport, Exercise and Rehabilitation Sciences, University of Birmingham, Birmingham,United Kingdom. FAU - Yu, Jinglei AU - Yu J AD - School of Biosciences, University of Birmingham, Birmingham,United Kingdom. FAU - Philp, Andrew AU - Philp A AD - Centre for Healthy Ageing, Centenary Institute, Sydney, NSW,Australia. FAU - Baar, Keith AU - Baar K AD - Department of Neurobiology, Physiology and Behavior, University of California, Davis, CA,USA. FAU - Tipton, Kevin D AU - Tipton KD AD - Institute for Performance Nutrition, Edinburgh,Scotland. AD - Research Institute for Sport and Exercise Sciences, Liverpool John Moores University, Liverpool,United Kingdom. FAU - Witard, Oliver C AU - Witard OC AD - Centre for Human and Applied Physiological Sciences, Faculty of Life Sciences and Medicine, King's College London, London,United Kingdom. LA - eng PT - Journal Article DEP - 20230524 PL - United States TA - Int J Sport Nutr Exerc Metab JT - International journal of sport nutrition and exercise metabolism JID - 100939812 RN - 0 (Amino Acids, Branched-Chain) RN - 0 (Dietary Carbohydrates) RN - GMW67QNF9C (Leucine) SB - IM MH - Male MH - Humans MH - *Amino Acids, Branched-Chain MH - *Resistance Training MH - Dietary Carbohydrates/metabolism MH - Leucine MH - Eating MH - Muscle, Skeletal/metabolism OTO - NOTNLM OT - fractional synthetic rate OT - leucine OT - muscle anabolism EDAT- 2023/05/25 01:07 MHDA- 2023/06/23 06:42 CRDT- 2023/05/24 19:52 PHST- 2023/01/28 00:00 [received] PHST- 2023/04/05 00:00 [revised] PHST- 2023/04/07 00:00 [accepted] PHST- 2023/06/23 06:42 [medline] PHST- 2023/05/25 01:07 [pubmed] PHST- 2023/05/24 19:52 [entrez] AID - 10.1123/ijsnem.2023-0015 [doi] PST - epublish SO - Int J Sport Nutr Exerc Metab. 2023 May 24;33(4):189-197. doi: 10.1123/ijsnem.2023-0015. Print 2023 Jul 1.