PMID- 37226366
OWN - NLM
STAT- MEDLINE
DCOM- 20230706
LR  - 20230706
IS  - 1600-0765 (Electronic)
IS  - 0022-3484 (Linking)
VI  - 58
IP  - 4
DP  - 2023 Aug
TI  - EGF coating of titanium surfaces modulates cytokines in oral mucosal primary 
      cells exposed to TNF-alpha.
PG  - 791-799
LID - 10.1111/jre.13138 [doi]
AB  - OBJECTIVE: This study assessed the metabolism of oral mucosal cells cultured on 
      titanium discs (Ti) coated (or not) with epidermal growth factor (EGF) and 
      exposed to tumor necrosis factor alpha (TNF-alpha). METHODS: Fibroblasts or 
      keratinocytes were seeded on Ti coated or not with EGF, and then exposed to 
      100 ng/mL of TNF-alpha for 24 h. Groups were established: G1: Ti (control); G2: 
      Ti + TNF-alpha; G3: Ti + EGF; and G4: Ti + EGF + TNF-alpha. Both cell lines were 
      evaluated for: viability (AlamarBlue(R), n = 8); interleukin 6 and 8 (IL-6, IL-8) 
      gene expression (qPCR, n = 5), and protein synthesis (ELISA, n = 6). For 
      keratinocytes cells, the matrix metalloproteinase type 3 (MMP-3) was evaluated by 
      qPCR (n = 5) and ELISA (n = 6). A 3-D culture of fibroblasts was analyzed by 
      confocal microscopy. The data were subjected to ANOVA analysis, alpha = 5%. RESULTS: 
      Increased cell viability was observed in all groups compared with G1. Enhanced 
      gene expression and synthesis of IL-6 and IL-8 by fibroblasts and keratinocytes 
      in G2 and modulation of hIL-6 gene expression in G4 was noted. Modulation of IL-8 
      synthesis occurred in keratinocytes in G3 and G4. Keratinocytes in G2 showed 
      enhanced gene expression of hMMP-3. A 3-D culture showed more cells in G3. 
      Fibroblasts in G2 exhibited disrupted cytoplasmic membrane. Cells in G4 showed 
      elongated morphology with intact cytoplasm. CONCLUSIONS: EGF coating increases 
      cell viability and modulates the response of oral cells exposed to an 
      inflammatory stimulus.
CI  - (c) 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Pansani, Taisa Nogueira
AU  - Pansani TN
AD  - Department of Physiology and Pathology, Sao Paulo State University (UNESP), 
      Araraquara School of Dentistry, Araraquara, Brazil.
FAU - Basso, Fernanda Goncalves
AU  - Basso FG
AUID- ORCID: 0000-0002-7170-2371
AD  - Department of Dentistry, Universidade de Ribeirao Preto, UNAERP, Ribeirao Preto, 
      Brazil.
FAU - Cardoso, Lais Medeiros
AU  - Cardoso LM
AD  - Department of Dental Materials and Prosthodontics, Sao Paulo State University 
      (UNESP), Araraquara School of Dentistry, Araraquara, Brazil.
FAU - de Souza Costa, Carlos Alberto
AU  - de Souza Costa CA
AD  - Department of Physiology and Pathology, Sao Paulo State University (UNESP), 
      Araraquara School of Dentistry, Araraquara, Brazil.
LA  - eng
GR  - 302047/2019-0/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/
GR  - 408721/2018-9/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/
GR  - 2020/09095-8/Sao Paulo Research Foundation-FAPESP/
GR  - 2019/20783-6/Sao Paulo Research Foundation-FAPESP/
PT  - Journal Article
DEP - 20230524
PL  - United States
TA  - J Periodontal Res
JT  - Journal of periodontal research
JID - 0055107
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 0 (Cytokines)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - D1JT611TNE (Titanium)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
SB  - IM
MH  - *Epidermal Growth Factor/pharmacology
MH  - *Cytokines
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - Titanium/pharmacology
MH  - Interleukin-6
MH  - Interleukin-8
MH  - Cells, Cultured
MH  - Fibroblasts
OTO - NOTNLM
OT  - epidermal growth factor
OT  - fibroblasts
OT  - keratinocytes
OT  - titanium
OT  - tumor necrosis factor alpha
EDAT- 2023/05/25 06:42
MHDA- 2023/07/06 06:42
CRDT- 2023/05/25 00:53
PHST- 2023/03/30 00:00 [revised]
PHST- 2022/09/22 00:00 [received]
PHST- 2023/05/06 00:00 [accepted]
PHST- 2023/07/06 06:42 [medline]
PHST- 2023/05/25 06:42 [pubmed]
PHST- 2023/05/25 00:53 [entrez]
AID - 10.1111/jre.13138 [doi]
PST - ppublish
SO  - J Periodontal Res. 2023 Aug;58(4):791-799. doi: 10.1111/jre.13138. Epub 2023 May 
      24.