PMID- 37226739
OWN - NLM
STAT- MEDLINE
DCOM- 20240201
LR  - 20240206
IS  - 1532-2378 (Electronic)
IS  - 1049-5398 (Linking)
VI  - 34
IP  - 8
DP  - 2023 Dec
TI  - Diverse pathogen-associated molecular patterns affect transcription of genes in 
      the toll-like receptor signaling pathway in goat blood.
PG  - 3729-3738
LID - 10.1080/10495398.2023.2214189 [doi]
AB  - Pathogen-associated molecular patterns (PAMPs) such as lipopolysaccharide (LPS), 
      peptidoglycan (PGN), Polyinosinic-polycytidylic acid (poly I:C), and CpG 
      Oligodeoxynucleotides (ODN) are recognized by Toll-like receptors (TLR). This 
      study aimed to investigate the effect of diverse PAMPs on the transcription of 
      TLR signaling pathway genes in goat blood. Whole blood was collected from 3 
      female BoerXSpanish goats and treated with the following PAMPs: 10 microg/ml LPS, 
      PGN, CpG ODN (2216), CpG ODN (2006), and 12.5 microg/ml Poly I:C. Blood-treated PBS 
      served as a control. The expression of 84 genes in the human TLR signaling 
      pathway RT2 PCR Array (Qiagen) was evaluated using real-time PCR. Treatment with 
      PBS affected the expression of 74 genes, Poly I:C affected the expression of 40 
      genes, t ODN 2006 affected the expression of 50 genes, ODN 2216 affected the 
      expression of 52 genes, LPS affected the expression of 49 genes, while PGN 
      affected the expression of 49 genes. Our results show that PAMPs modulated and 
      increased the expression of genes in the TLR signaling pathway. These results 
      highlight important insights into how the host responds to different pathogens 
      and may help design adjuvants for therapeutics and vaccines that 
      target different.
FAU - Ekwemalor, Kingsley
AU  - Ekwemalor K
AD  - Department of Animal Sciences, North Carolina Agricultural and Technical State 
      University, Greensboro, NC, USA.
FAU - Asiamah, Emmanuel
AU  - Asiamah E
AD  - Department of Agriculture, University of Arkansas at Pine Bluff, Pine Bluff, AR, 
      USA.
FAU - Adjei-Fremah, Sarah
AU  - Adjei-Fremah S
AD  - Department of Biological Sciences, Winston-Salem State University, Winston-Salem, 
      NC, USA.
FAU - Eluka-Okoludoh, Eboghoye
AU  - Eluka-Okoludoh E
AD  - Department of Animal Sciences, North Carolina Agricultural and Technical State 
      University, Greensboro, NC, USA.
FAU - Mulakala, Bharath
AU  - Mulakala B
AD  - Department of Animal Sciences, North Carolina Agricultural and Technical State 
      University, Greensboro, NC, USA.
FAU - Osei, Bertha
AU  - Osei B
AD  - Department of Animal Sciences, North Carolina Agricultural and Technical State 
      University, Greensboro, NC, USA.
FAU - Worku, Mulumebet
AU  - Worku M
AD  - Department of Animal Sciences, North Carolina Agricultural and Technical State 
      University, Greensboro, NC, USA.
LA  - eng
PT  - Journal Article
DEP - 20230525
PL  - England
TA  - Anim Biotechnol
JT  - Animal biotechnology
JID - 9011409
RN  - 0 (Pathogen-Associated Molecular Pattern Molecules)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Toll-Like Receptors)
RN  - 25249-22-3 (Poly I)
SB  - IM
MH  - Female
MH  - Animals
MH  - Humans
MH  - *Pathogen-Associated Molecular Pattern Molecules
MH  - *Lipopolysaccharides/pharmacology
MH  - Goats/genetics/metabolism
MH  - Toll-Like Receptors/genetics/metabolism
MH  - Signal Transduction/genetics
MH  - Poly I
OTO - NOTNLM
OT  - LPS
OT  - PAMP
OT  - Toll-like receptors
OT  - gene expression
OT  - peptidoglycan PGN
OT  - poly IC
EDAT- 2023/05/25 06:42
MHDA- 2024/02/01 06:42
CRDT- 2023/05/25 05:34
PHST- 2024/02/01 06:42 [medline]
PHST- 2023/05/25 06:42 [pubmed]
PHST- 2023/05/25 05:34 [entrez]
AID - 10.1080/10495398.2023.2214189 [doi]
PST - ppublish
SO  - Anim Biotechnol. 2023 Dec;34(8):3729-3738. doi: 10.1080/10495398.2023.2214189. 
      Epub 2023 May 25.