PMID- 37227271
OWN - NLM
STAT- MEDLINE
DCOM- 20230619
LR  - 20231120
IS  - 2165-0497 (Electronic)
IS  - 2165-0497 (Linking)
VI  - 11
IP  - 3
DP  - 2023 Jun 15
TI  - Epidemiological Investigation, Risk Factors, Spatial-Temporal Cluster, and 
      Epidemic Trend Analysis of Pseudorabies Virus Seroprevalence in China (2017 to 
      2021).
PG  - e0529722
LID - 10.1128/spectrum.05297-22 [doi]
LID - e05297-22
AB  - Pseudorabies virus (PRV) is a double-stranded linear DNA virus capable of 
      infecting various animals, including humans. We collected blood samples from 14 
      provinces in China between December 2017 and May 2021 to estimate PRV 
      seroprevalence. The PRV gE antibody was detected using the enzyme-linked 
      immunosorbent assay (ELISA). Logistic regression analysis identified potential 
      risk factors associated with PRV gE serological status at the farm level. 
      Spatial-temporal clusters of high PRV gE seroprevalence were explored using 
      SaTScan 9.6 software. Time-series data of PRV gE seroprevalence were modeled 
      using the autoregressive moving average (ARMA) method. A Monte Carlo sampling 
      simulation based on the established model was performed to analyze epidemic 
      trends of PRV gE seroprevalence using @RISK software (version 7.0). A total of 
      40,024 samples were collected from 545 pig farms across China. The PRV gE 
      antibody positivity rates were 25.04% (95% confidence interval [CI], 24.61% to 
      25.46%) at the animal level and 55.96% (95% CI, 51.68% to 60.18%) at the pig farm 
      level. Variables such as farm geographical division, farm topography, African 
      swine fever (ASF) outbreak, and porcine reproductive and respiratory syndrome 
      virus (PRRSV) control in pig farms were identified as risk factors for farm-level 
      PRV infection. Five significant high-PRV gE seroprevalence clusters were detected 
      in China for the first time, with a time range of 1 December 2017 to 31 July 
      2019. The monthly average change value of PRV gE seroprevalence was -0.826%. The 
      probability of a monthly PRV gE seroprevalence decrease was 0.868, while an 
      increase was 0.132. IMPORTANCE PRV is a critical pathogen threatening the global 
      swine industry. Our research fills knowledge gaps regarding PRV prevalence, 
      infection risk factors, spatial-temporal clustering of high PRV gE 
      seroprevalence, and the epidemic trend of PRV gE seroprevalence in China in 
      recent years. These findings are valuable for the clinical prevention and control 
      of PRV infection and suggest that PRV infection is likely to be successfully 
      controlled in China.
FAU - Zhao, Pengfei
AU  - Zhao P
AD  - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural 
      University. Wuhan, Hubei, People's Republic of China.
FAU - Wang, Yu
AU  - Wang Y
AD  - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural 
      University. Wuhan, Hubei, People's Republic of China.
FAU - Zhang, Pengfei
AU  - Zhang P
AD  - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural 
      University. Wuhan, Hubei, People's Republic of China.
FAU - Du, Fen
AU  - Du F
AD  - Hubei Center for Animal Disease Control and Prevention, Wuhan, Hubei, People's 
      Republic of China.
FAU - Li, Jianhai
AU  - Li J
AD  - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural 
      University. Wuhan, Hubei, People's Republic of China.
FAU - Wang, Chaofei
AU  - Wang C
AD  - Wuhan Keweichuang Biotechnology Co., Ltd., Wuhan, Hubei, People's Republic of 
      China.
FAU - Fang, Rui
AU  - Fang R
AUID- ORCID: 0000-0003-0020-8488
AD  - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural 
      University. Wuhan, Hubei, People's Republic of China.
FAU - Zhao, Junlong
AU  - Zhao J
AUID- ORCID: 0000-0001-5162-4495
AD  - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural 
      University. Wuhan, Hubei, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230525
PL  - United States
TA  - Microbiol Spectr
JT  - Microbiology spectrum
JID - 101634614
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Humans
MH  - Swine
MH  - Animals
MH  - *Herpesvirus 1, Suid/genetics
MH  - *Pseudorabies/epidemiology/prevention & control
MH  - *African Swine Fever
MH  - Seroepidemiologic Studies
MH  - *Swine Diseases/epidemiology
MH  - China/epidemiology
MH  - Risk Factors
MH  - Antibodies, Viral
PMC - PMC10269690
OTO - NOTNLM
OT  - China
OT  - epidemic trend analysis
OT  - epidemiological investigation
OT  - pseudorabies virus
OT  - risk factors
OT  - seroprevalence
OT  - spatial-temporal clustering
COIS- The authors declare no conflict of interest.
EDAT- 2023/05/25 13:07
MHDA- 2023/06/19 13:09
PMCR- 2023/05/25
CRDT- 2023/05/25 10:04
PHST- 2023/06/19 13:09 [medline]
PHST- 2023/05/25 13:07 [pubmed]
PHST- 2023/05/25 10:04 [entrez]
PHST- 2023/05/25 00:00 [pmc-release]
AID - 05297-22 [pii]
AID - spectrum.05297-22 [pii]
AID - 10.1128/spectrum.05297-22 [doi]
PST - ppublish
SO  - Microbiol Spectr. 2023 Jun 15;11(3):e0529722. doi: 10.1128/spectrum.05297-22. 
      Epub 2023 May 25.