PMID- 37228640
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230528
IS  - 2639-5274 (Electronic)
IS  - 2639-5274 (Linking)
VI  - 6
DP  - 2023
TI  - Magnetic Fields Reduce Apoptosis by Suppressing Phase Separation of Tau-441.
PG  - 0146
LID - 10.34133/research.0146 [doi]
LID - 0146
AB  - The biological effects of magnetic fields (MFs) have been a controversial issue. 
      Fortunately, in recent years, there has been increasing evidence that MFs do 
      affect biological systems. However, the physical mechanism remains unclear. Here, 
      we show that MFs (16 T) reduce apoptosis in cell lines by inhibiting 
      liquid-liquid phase separation (LLPS) of Tau-441, suggesting that the MF effect 
      on LLPS may be one of the mechanisms for understanding the "mysterious" 
      magnetobiological effects. The LLPS of Tau-441 occurred in the cytoplasm after 
      induction with arsenite. The phase-separated droplets of Tau-441 recruited 
      hexokinase (HK), resulting in a decrease in the amount of free HK in the 
      cytoplasm. In cells, HK and Bax compete to bind to the voltage-dependent anion 
      channel (VDAC I) on the mitochondrial membrane. A decrease in the number of free 
      HK molecules increased the chance of Bax binding to VDAC I, leading to increased 
      Bax-mediated apoptosis. In the presence of a static MF, LLPS was marked inhibited 
      and HK recruitment was reduced, resulting in an increased probability of HK 
      binding to VDAC I and a decreased probability of Bax binding to VDAC I, thus 
      reducing Bax-mediated apoptosis. Our findings revealed a new physical mechanism 
      for understanding magnetobiological effects from the perspective of LLPS. In 
      addition, these results show the potential applications of physical environments, 
      such as MFs in this study, in the treatment of LLPS-related diseases.
CI  - Copyright (c) 2023 Wen-Juan Lin et al.
FAU - Lin, Wen-Juan
AU  - Lin WJ
AD  - Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, 
      Northwestern Polytechnical University, 127 Youyixi Road, Xi'an 710072, Shaanxi, 
      PR China.
FAU - Shi, Wen-Pu
AU  - Shi WP
AD  - Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, 
      Northwestern Polytechnical University, 127 Youyixi Road, Xi'an 710072, Shaanxi, 
      PR China.
FAU - Ge, Wan-Yi
AU  - Ge WY
AD  - Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, 
      Northwestern Polytechnical University, 127 Youyixi Road, Xi'an 710072, Shaanxi, 
      PR China.
FAU - Chen, Liang-Liang
AU  - Chen LL
AD  - Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, 
      Northwestern Polytechnical University, 127 Youyixi Road, Xi'an 710072, Shaanxi, 
      PR China.
FAU - Guo, Wei-Hong
AU  - Guo WH
AD  - Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, 
      Northwestern Polytechnical University, 127 Youyixi Road, Xi'an 710072, Shaanxi, 
      PR China.
FAU - Shang, Peng
AU  - Shang P
AD  - Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, 
      Northwestern Polytechnical University, 127 Youyixi Road, Xi'an 710072, Shaanxi, 
      PR China.
FAU - Yin, Da-Chuan
AU  - Yin DC
AD  - Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, 
      Northwestern Polytechnical University, 127 Youyixi Road, Xi'an 710072, Shaanxi, 
      PR China.
LA  - eng
PT  - Journal Article
DEP - 20230511
PL  - United States
TA  - Research (Wash D C)
JT  - Research (Washington, D.C.)
JID - 101747148
PMC - PMC10204748
EDAT- 2023/05/25 19:12
MHDA- 2023/05/25 19:13
PMCR- 2023/05/11
CRDT- 2023/05/25 14:12
PHST- 2022/10/31 00:00 [received]
PHST- 2023/04/21 00:00 [accepted]
PHST- 2023/05/25 19:13 [medline]
PHST- 2023/05/25 19:12 [pubmed]
PHST- 2023/05/25 14:12 [entrez]
PHST- 2023/05/11 00:00 [pmc-release]
AID - 0146 [pii]
AID - 10.34133/research.0146 [doi]
PST - epublish
SO  - Research (Wash D C). 2023 May 11;6:0146. doi: 10.34133/research.0146. eCollection 
      2023.