PMID- 37229651
OWN - NLM
STAT- MEDLINE
DCOM- 20230607
LR  - 20230627
IS  - 1945-4589 (Electronic)
IS  - 1945-4589 (Linking)
VI  - 15
IP  - 10
DP  - 2023 May 23
TI  - Mechanisms of human umbilical cord mesenchymal stem cells-derived exosomal lncRNA 
      GAS5 in alleviating EMT of HPMCs via Wnt/beta-catenin signaling pathway.
PG  - 4144-4158
LID - 10.18632/aging.204719 [doi]
AB  - BACKGROUND: Prolonged peritoneal dialysis (PD) can result in 
      epithelial-to-mesenchymal transition (EMT) and peritoneal fibrosis (PF), which 
      can cause patients to discontinue PD. It is imperative to urgently investigate 
      effective measures to mitigate PF. This study aims to reveal mechanisms of 
      exosomal lncRNA GAS5 derived from human umbilical cord mesenchymal stem cells 
      (hUC-MSCs) on EMT of human peritoneal mesothelial cells (HPMCs) under high 
      glucose (HG) conditions. METHODS: HPMCs were stimulated with 2.5% glucose. The 
      effects on EMT of HPMCs were observed by using an hUC-MSC conditioned medium 
      (hUC-MSC-CM) and extracted exosomes. After hUC-MSCs were transfected with GAS5 
      siRNA, exosomes were extracted to act on HPMCs for detecting EMT markers, PTEN, 
      and Wnt/beta-catenin pathway, lncRNA GAS5 and miR-21 expressions in HPMCs. RESULTS: 
      We found that HG could induce the EMT of HPMCs. Compared with the HG group, the 
      hUC-MSC-CM could alleviate the EMT of HPMCs induced by HG through exosomes. 
      Exosomes in the hUC-MSC-CM entered HPMCs, by transferring lncRNA GAS5 to HPMCs, 
      which down-regulates miR-21 and up-regulates PTEN, thus finally alleviating EMT 
      of HPMCs. The Wnt/beta-catenin pathway plays an essential role in alleviating EMT of 
      HPMCs by exosomes in the hUC-MSC-CM. By transferring lncRNA GAS5 to HPMCs, 
      exosomes derived from hUC-MSCs may competitively bind to miR-21 to regulate 
      suppression on target PTEN genes and alleviate EMT of HPMCs through the 
      Wnt/beta-catenin pathway. CONCLUSIONS: Exosomes from the hUC-MSCs-CM could alleviate 
      the EMT of HPMCs induced by HG via regulating lncRNA GAS5/miR-21/PTEN through the 
      Wnt/beta-catenin signaling pathway.
FAU - Huang, Yuling
AU  - Huang Y
AD  - Department of Geriatrics, The First Hospital of China Medical University, 
      Shenyang, Liaoning 110001, China.
FAU - Ma, Jianfei
AU  - Ma J
AD  - Department of Nephrology, The First Hospital of China Medical University, 
      Shenyang, Liaoning 110001, China.
FAU - Fan, Yi
AU  - Fan Y
AD  - Department of Nephrology, The First Hospital of China Medical University, 
      Shenyang, Liaoning 110001, China.
FAU - Yang, Lina
AU  - Yang L
AD  - Department of Geriatrics, The First Hospital of China Medical University, 
      Shenyang, Liaoning 110001, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230523
PL  - United States
TA  - Aging (Albany NY)
JT  - Aging
JID - 101508617
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (beta Catenin)
RN  - 0 (MicroRNAs)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Humans
MH  - Wnt Signaling Pathway
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - beta Catenin/metabolism
MH  - *MicroRNAs/genetics/metabolism
MH  - Glucose/metabolism
MH  - *Mesenchymal Stem Cells/metabolism
MH  - Umbilical Cord/metabolism
PMC - PMC10258012
OTO - NOTNLM
OT  - epithelial-mesenchymal transition
OT  - exosome
OT  - human peritoneal mesothelial cell
OT  - human umbilical cord mesenchymal stem cell
OT  - peritoneal dialysis
COIS- CONFLICTS OF INTEREST: The authors declare no conflicts of interest related to 
      this study.
EDAT- 2023/05/25 19:12
MHDA- 2023/06/07 06:42
PMCR- 2023/05/31
CRDT- 2023/05/25 16:43
PHST- 2023/01/08 00:00 [received]
PHST- 2023/05/01 00:00 [accepted]
PHST- 2023/06/07 06:42 [medline]
PHST- 2023/05/25 19:12 [pubmed]
PHST- 2023/05/25 16:43 [entrez]
PHST- 2023/05/31 00:00 [pmc-release]
AID - 204719 [pii]
AID - 10.18632/aging.204719 [doi]
PST - ppublish
SO  - Aging (Albany NY). 2023 May 23;15(10):4144-4158. doi: 10.18632/aging.204719. Epub 
      2023 May 23.