PMID- 37231123
OWN - NLM
STAT- MEDLINE
DCOM- 20230922
LR  - 20231201
IS  - 1432-198X (Electronic)
IS  - 0931-041X (Print)
IS  - 0931-041X (Linking)
VI  - 38
IP  - 11
DP  - 2023 Nov
TI  - Intrarenal renin-angiotensin system activation and macrophage infiltrations in 
      pediatric chronic glomerulonephritis.
PG  - 3711-3719
LID - 10.1007/s00467-023-06026-5 [doi]
AB  - BACKGROUND: The current study tested the hypothesis that urinary angiotensinogen 
      (UAGT) and urinary monocyte chemoattractant protein-1 (UMCP-1) levels provide a 
      specific index of intrarenal renin-angiotensin system (RAS) status and the degree 
      of infiltration of macrophages associated with RAS blockade and immunosuppressant 
      treatment in pediatric patients with chronic glomerulonephritis. METHODS: We 
      measured baseline UAGT and UMCP-1 levels to examine the correlation between 
      glomerular injury in 48 pediatric chronic glomerulonephritis patients before 
      treatment. Furthermore, we performed immunohistochemical analysis of 
      angiotensinogen (AGT) and CD68 in 27 pediatric chronic glomerulonephritis 
      patients treated with RAS blockades and immunosuppressants for 2 years. Finally, 
      we examined the effects of angiotensin II (Ang II) on monocyte chemoattractant 
      protein-1 (MCP-1) expression in cultured human mesangial cells (MCs). RESULTS: 
      Baseline UAGT and UMCP-1 levels positively correlated with urinary protein 
      levels, scores for mesangial hypercellularity, rate of crescentic formation, and 
      expression levels of AGT and CD68 in renal tissues (p < 0.05). UAGT and UMCP-1 
      levels were significantly decreased after RAS blockade and immunosuppressant 
      treatment (p < 0.01), which was accompanied by AGT and CD68 (p < 0.01), as well 
      as the magnitude of glomerular injury. Cultured human MCs showed increased MCP-1 
      messenger ribonucleic acid and protein levels after Ang II treatment (p < 0.01). 
      CONCLUSIONS: The data indicates that UAGT and UMCP-1 are useful biomarkers of the 
      degree of glomerular injury during RAS blockade and immunosuppressant treatment 
      in pediatric patients with chronic glomerulonephritis.
CI  - (c) 2023. The Author(s).
FAU - Hattori, Tomoki
AU  - Hattori T
AD  - Department of Pediatrics, Tokushima University Graduate School of Biomedical 
      Sciences, Kuramoto-cho 3-18-15, Tokushima, Tokushima, 770-8503, Japan.
AD  - Department of Pediatrics, Mie University Graduate School of Medicine, Mie, Japan.
FAU - Fujioka, Keisuke
AU  - Fujioka K
AD  - Department of Pediatrics, Tokushima University Graduate School of Biomedical 
      Sciences, Kuramoto-cho 3-18-15, Tokushima, Tokushima, 770-8503, Japan.
FAU - Nagai, Takashi
AU  - Nagai T
AD  - Department of Pediatrics, Tokushima University Graduate School of Biomedical 
      Sciences, Kuramoto-cho 3-18-15, Tokushima, Tokushima, 770-8503, Japan.
FAU - Kondo, Shuji
AU  - Kondo S
AD  - Department of Pediatrics, Tokushima University Graduate School of Biomedical 
      Sciences, Kuramoto-cho 3-18-15, Tokushima, Tokushima, 770-8503, Japan.
FAU - Kagami, Shoji
AU  - Kagami S
AD  - Department of Pediatrics, Tokushima University Graduate School of Biomedical 
      Sciences, Kuramoto-cho 3-18-15, Tokushima, Tokushima, 770-8503, Japan.
FAU - Hirayama, Masahiro
AU  - Hirayama M
AD  - Department of Pediatrics, Mie University Graduate School of Medicine, Mie, Japan.
FAU - Urushihara, Maki
AU  - Urushihara M
AUID- ORCID: 0000-0002-6431-3239
AD  - Department of Pediatrics, Tokushima University Graduate School of Biomedical 
      Sciences, Kuramoto-cho 3-18-15, Tokushima, Tokushima, 770-8503, Japan. 
      urushihara@tokushima-u.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230525
PL  - Germany
TA  - Pediatr Nephrol
JT  - Pediatric nephrology (Berlin, Germany)
JID - 8708728
RN  - 11002-13-4 (Angiotensinogen)
RN  - 0 (Chemokine CCL2)
RN  - 11128-99-7 (Angiotensin II)
RN  - 0 (Immunosuppressive Agents)
SB  - IM
MH  - Humans
MH  - Child
MH  - *Renin-Angiotensin System
MH  - Angiotensinogen/urine
MH  - Kidney/metabolism
MH  - Chemokine CCL2
MH  - *Glomerulonephritis/metabolism
MH  - Angiotensin II/metabolism
MH  - Chronic Disease
MH  - Immunosuppressive Agents/pharmacology/therapeutic use
MH  - Macrophages/metabolism
PMC - PMC10514104
OTO - NOTNLM
OT  - Angiotensinogen
OT  - CD68
OT  - Monocyte chemoattractant protein-1
OT  - Pediatric chronic glomerulonephritis
OT  - Renin-angiotensin system
COIS- The authors have no competing interests to declare that are relevant to the 
      content of this article.
EDAT- 2023/05/26 01:05
MHDA- 2023/09/22 06:43
PMCR- 2023/05/25
CRDT- 2023/05/25 23:25
PHST- 2022/12/26 00:00 [received]
PHST- 2023/04/25 00:00 [accepted]
PHST- 2023/04/21 00:00 [revised]
PHST- 2023/09/22 06:43 [medline]
PHST- 2023/05/26 01:05 [pubmed]
PHST- 2023/05/25 23:25 [entrez]
PHST- 2023/05/25 00:00 [pmc-release]
AID - 10.1007/s00467-023-06026-5 [pii]
AID - 6026 [pii]
AID - 10.1007/s00467-023-06026-5 [doi]
PST - ppublish
SO  - Pediatr Nephrol. 2023 Nov;38(11):3711-3719. doi: 10.1007/s00467-023-06026-5. Epub 
      2023 May 25.