PMID- 37233461 OWN - NLM STAT- MEDLINE DCOM- 20230529 LR - 20230529 IS - 1660-3397 (Electronic) IS - 1660-3397 (Linking) VI - 21 IP - 5 DP - 2023 Apr 26 TI - Proliferative Effect of Aqueous Extract of Sea Cucumber (Holothuria parva) Body Wall on Human Umbilical Cord Mesenchymal Stromal/Stem Cells. LID - 10.3390/md21050267 [doi] LID - 267 AB - Sea cucumber extracts and their bioactive compounds have the potential for stem cell proliferation induction and for their beneficial therapeutic properties. In this study, human umbilical cord mesenchymal stromal/stem cells (hUC-MSCs) were exposed to an aqueous extract of Holothuria parva body walls. Proliferative molecules were detected using gas chromatography-mass spectrometry (GC-MS) analysis in an aqueous extract of H. parva. The aqueous extract concentrations of 5, 10, 20, 40, and 80 microg/mL and 10 and 20 ng/mL of human epidermal growth factor (EGF) as positive controls were treated on hUC-MSCs. MTT, cell count, viability, and cell cycle assays were performed. Using Western blot analysis, the effects of extracts of H. parva and EGF on cell proliferation markers were detected. Computational modeling was done to detect effective proliferative compounds in the aqueous extract of H. parva. A MTT assay showed that the 10, 20, and 40 microg/mL aqueous extract of H. parva had a proliferative effect on hUC-MSCs. The cell count, which was treated with a 20 microg/mL concentration, increased faster and higher than the control group (p < 0.05). This concentration of the extract did not have a significant effect on hUC-MSCs' viability. The cell cycle assay of hUC-MSCs showed that the percentage of cells in the G2 stage of the extract was biologically higher than the control group. Expression of cyclin D1, cyclin D3, cyclin E, HIF-1alpha, and TERT was increased compared with the control group. Moreover, expression of p21 and PCNA decreased after treating hUC-MSCs with the extract. However, CDC-2/cdk-1 and ERK1/2 had almost the same expression as the control group. The expression of CDK-4 and CDK-6 decreased after treatment. Between the detected compounds, 1-methyl-4-(1-methyl phenyl)-benzene showed better affinity to CDK-4 and p21 than tetradecanoic acid. The H. parva aqueous extract showed proliferative potential on hUC-MSCs. FAU - Rasekh, Poorya AU - Rasekh P AUID- ORCID: 0000-0002-3373-0021 AD - The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr 7514633196, Iran. FAU - Kameli, Ali AU - Kameli A AD - The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr 7514633196, Iran. FAU - Khoradmehr, Arezoo AU - Khoradmehr A AD - The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr 7514633196, Iran. FAU - Baghban, Neda AU - Baghban N AUID- ORCID: 0000-0002-3932-6978 AD - The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr 7514633196, Iran. FAU - Mohebbi, Gholamhossein AU - Mohebbi G AD - The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr 7514633196, Iran. FAU - Barmak, Alireza AU - Barmak A AD - Food Lab, Bushehr University of Medical Sciences, Bushehr 7518759577, Iran. FAU - Nabipour, Iraj AU - Nabipour I AD - The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr 7514633196, Iran. FAU - Azari, Hossein AU - Azari H AD - The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr 7514633196, Iran. FAU - Heidari, Yaser AU - Heidari Y AD - The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr 7514633196, Iran. FAU - Daneshi, Adel AU - Daneshi A AD - The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr 7514633196, Iran. FAU - Bargahi, Afshar AU - Bargahi A AD - The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr 7514633196, Iran. FAU - Khodabandeh, Zahra AU - Khodabandeh Z AD - Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz 71348-14336, Iran. FAU - Zare, Shahrokh AU - Zare S AD - Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz 71348-14336, Iran. FAU - Afshar, Alireza AU - Afshar A AUID- ORCID: 0000-0002-8618-5872 AD - The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr 7514633196, Iran. FAU - Shirazi, Reza AU - Shirazi R AD - Department of Anatomy, School of Medical Sciences, Medicine, UNSW Sydney, Sydney 3052, Australia. FAU - Almasi-Turk, Sahar AU - Almasi-Turk S AD - Department of Anatomical Sciences, School of Medicine, Bushehr University of Medical Sciences, Bushehr 7514633196, Iran. FAU - Tamadon, Amin AU - Tamadon A AUID- ORCID: 0000-0002-0222-3035 AD - PerciaVista R&D Co., Shiraz 7167683745, Iran. AD - Department for Scientific Work, West Kazakhstan Marat Ospanov Medical University, Aktobe 030012, Kazakhstan. LA - eng GR - 1271/Bushehr University of Medical Sciences/ PT - Journal Article DEP - 20230426 PL - Switzerland TA - Mar Drugs JT - Marine drugs JID - 101213729 RN - 62229-50-9 (Epidermal Growth Factor) SB - IM MH - Animals MH - Humans MH - Epidermal Growth Factor/pharmacology MH - *Holothuria MH - Cell Differentiation MH - *Sea Cucumbers MH - Umbilical Cord MH - Stem Cells PMC - PMC10220604 OTO - NOTNLM OT - aqueous extract OT - bioactive compounds OT - epidermal growth factor OT - proliferation OT - sea cucumbers OT - stem cells COIS- The author Amin Tamadon was employed by PerciaVista R&D Co. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2023/05/26 13:09 MHDA- 2023/05/29 06:42 PMCR- 2023/04/26 CRDT- 2023/05/26 10:12 PHST- 2022/11/13 00:00 [received] PHST- 2022/11/25 00:00 [revised] PHST- 2022/11/25 00:00 [accepted] PHST- 2023/05/29 06:42 [medline] PHST- 2023/05/26 13:09 [pubmed] PHST- 2023/05/26 10:12 [entrez] PHST- 2023/04/26 00:00 [pmc-release] AID - md21050267 [pii] AID - marinedrugs-21-00267 [pii] AID - 10.3390/md21050267 [doi] PST - epublish SO - Mar Drugs. 2023 Apr 26;21(5):267. doi: 10.3390/md21050267.