PMID- 37235301
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230530
IS  - 2414-6366 (Electronic)
IS  - 2414-6366 (Linking)
VI  - 8
IP  - 5
DP  - 2023 Apr 27
TI  - Eugenol Essential Oil and Nanoemulsion as Antihydatic Agents with Antifibrotic 
      and Immunomodulatory Effects in Cystic Echinococcosis.
LID - 10.3390/tropicalmed8050253 [doi]
LID - 253
AB  - Conventional scolicidal agents are still unsatisfactory in combating hydatid 
      disease due to their low efficacy and increased drug side effects. Therefore, 
      novel scolicides are required. This study aimed to evaluate the antihydatic and 
      immunomodulatory effects of eugenol essential oil (Eug) and its nanoemulsion 
      (Eug-NE) in cystic echinococcosis (CE). Eug and Eug-NE were administered orally 
      to CE-infected rats and compared to albendazole (ABZ). Hydatid cyst development 
      was assessed based on organ weight and hypertrophy indicators of the infected 
      organs, along with a histopathological and histochemical evaluation of collagen 
      content. The immunomodulatory effects of treatment on CE were evaluated by serum 
      cytokine levels measurement of interferon-gamma (IFN-gamma) and interleukin (IL)-4 and 
      immunohistochemical (IHC) analysis of signal transducer and activator of 
      transcription 4 (STAT4) and GATA-binding protein 3 (GATA3) markers. Eug-NE was 
      the most effective in reducing the cyst weights, organ weights, and hypertrophy 
      indicators and improving histopathological lesions with reduced collagen content. 
      Eug and Eug-NE significantly increased the IFN-gamma levels and decreased the IL-4 
      levels, while IHC analysis demonstrated a significant reduction in STAT4 and 
      GATA3 expression in all treated groups. Eug and Eug-NE demonstrated antihydatic 
      and preventative effects, with a substantial decrease in liver fibrosis compared 
      to that of ABZ. Besides their promising immunomodulatory effects, their good 
      treatment response suggests their use as alternatives or complementary scolicidal 
      agents in hydatid cyst treatment.
FAU - Ahmad, Alzahraa Abdelraouf
AU  - Ahmad AA
AUID- ORCID: 0000-0002-2947-3122
AD  - Department of Medical Parasitology, Faculty of Medicine, Assiut University, 
      Assiut 71515, Egypt.
FAU - Maurice, Maria Naged
AU  - Maurice MN
AD  - Department of Medical Parasitology, Faculty of Medicine, Assiut University, 
      Assiut 71515, Egypt.
FAU - Monib, Mohamed El-Salahy M
AU  - Monib MEM
AD  - Department of Medical Parasitology, Faculty of Medicine, Assiut University, 
      Assiut 71515, Egypt.
FAU - Soliman, Mahmoud
AU  - Soliman M
AD  - Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, 
      Assiut University, Assiut 71515, Egypt.
AD  - Department of Immunology, University of Texas Southwestern Medical Center, 
      Dallas, TX 75235, USA.
FAU - Al-Thagfan, Sultan S
AU  - Al-Thagfan SS
AD  - Department of Clinical and Hospital Pharmacy, College of Pharmacy, Taibah 
      University, Al Madinah Al Munawarah 30001, Saudi Arabia.
FAU - Huseein, Enas Abdelhameed Mahmoud
AU  - Huseein EAM
AD  - Department of Medical Parasitology, Faculty of Medicine, Assiut University, 
      Assiut 71515, Egypt.
LA  - eng
GR  - (Grant number: 2018-12)/faculty of Medicine, Assiut University/
PT  - Journal Article
DEP - 20230427
PL  - Switzerland
TA  - Trop Med Infect Dis
JT  - Tropical medicine and infectious disease
JID - 101709042
PMC - PMC10223691
OTO - NOTNLM
OT  - GATA3
OT  - STAT4
OT  - cystic echinococcosis
OT  - cytokine profile
OT  - eugenol
OT  - eugenol nanoemulsion
COIS- The authors declare no conflict of interest.
EDAT- 2023/05/26 19:14
MHDA- 2023/05/26 19:15
PMCR- 2023/04/27
CRDT- 2023/05/26 12:25
PHST- 2023/03/14 00:00 [received]
PHST- 2023/04/23 00:00 [revised]
PHST- 2023/04/25 00:00 [accepted]
PHST- 2023/05/26 19:15 [medline]
PHST- 2023/05/26 19:14 [pubmed]
PHST- 2023/05/26 12:25 [entrez]
PHST- 2023/04/27 00:00 [pmc-release]
AID - tropicalmed8050253 [pii]
AID - tropicalmed-08-00253 [pii]
AID - 10.3390/tropicalmed8050253 [doi]
PST - epublish
SO  - Trop Med Infect Dis. 2023 Apr 27;8(5):253. doi: 10.3390/tropicalmed8050253.