PMID- 37235408
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230530
IS  - 2306-7381 (Electronic)
IS  - 2306-7381 (Linking)
VI  - 10
IP  - 5
DP  - 2023 Apr 29
TI  - Genetic Variability and Recombination of the NSP2 Gene of PRRSV-2 Strains in 
      China from 1996 to 2021.
LID - 10.3390/vetsci10050325 [doi]
LID - 325
AB  - Porcine reproductive and respiratory syndrome (PRRS) is one of the most serious 
      infectious diseases that detrimentally affects the pig industry worldwide. The 
      disease, which is typically difficult to control, is an immunosuppressive disease 
      caused by the porcine reproductive and respiratory syndrome virus (PRRSV), the 
      genome of which (notably the NSP2 gene) undergoes rapid mutation. In this study, 
      we sought to determine the genetic variation in the PRRSV-2 NSP2 gene in China 
      from 1996 to 2021. Strain information was obtained from the GenBank database and 
      analyzed from a molecular epidemiological perspective. We compared the nucleotide 
      and amino acid homologies of the NSP2 sequences of different PRRSV-2 lineages, 
      and examined phylogenetic relationships based on an analysis of the NSP2 
      sequences of 122 strains. The results revealed that NADC-30-like strains, which 
      are represented by lineage 1, and HP-PRRSV strains, which are represented by 
      lineage 8, were the most prevalent in China from 1996 to 2021. Close similarities 
      were detected in the genetic evolution of lineages 3, 5, and 8. For nucleotide 
      and amino acid sequence comparisons, we selected representative strains from each 
      lineage, and for the NSP2 among different PRRSV-2 strains, we accordingly 
      detected homologies of 72.5-99.8% and 63.9-99.4% at the nucleotide and amino acid 
      levels, respectively, thereby indicating certain differences in the degrees of 
      NSP2 amino acid and nucleotide variation. Based on amino acid sequence 
      comparisons, we identified deletions, insertions, and substitutions at multiple 
      sites among the NSP2 sequences of PRRSV-2 strains. Recombination analysis 
      revealed the occurrence of five recombinant events among the 135 selected PRRSV-2 
      strains, and that there is a high probability of recombination of lineage 1 
      strains. The findings of this study enabled us to gain an in-depth understanding 
      of the prevalence of PRRSV in China over the past 25 years and will contribute to 
      providing a theoretical basis for evolution and epidemiology of the spread of 
      PRRSV.
FAU - Zhang, Hang
AU  - Zhang H
AUID- ORCID: 0009-0004-6294-8208
AD  - School of Life Science and Engineering, Foshan University, Foshan 528000, China.
FAU - Luo, Qin
AU  - Luo Q
AD  - School of Life Science and Engineering, Foshan University, Foshan 528000, China.
FAU - Zheng, Yajie
AU  - Zheng Y
AD  - School of Life Science and Engineering, Foshan University, Foshan 528000, China.
FAU - Sha, Huiyang
AU  - Sha H
AD  - School of Life Science and Engineering, Foshan University, Foshan 528000, China.
FAU - Li, Gan
AU  - Li G
AD  - School of Life Science and Engineering, Foshan University, Foshan 528000, China.
FAU - Kong, Weili
AU  - Kong W
AUID- ORCID: 0000-0002-9104-7730
AD  - Gladstone Institutes of Virology and Immunology, University of California, San 
      Francisco, CA 94158, USA.
FAU - Huang, Liangzong
AU  - Huang L
AD  - School of Life Science and Engineering, Foshan University, Foshan 528000, China.
FAU - Zhao, Mengmeng
AU  - Zhao M
AD  - School of Life Science and Engineering, Foshan University, Foshan 528000, China.
LA  - eng
GR  - 31902279/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20230429
PL  - Switzerland
TA  - Vet Sci
JT  - Veterinary sciences
JID - 101680127
PMC - PMC10224145
OTO - NOTNLM
OT  - NSP2 gene
OT  - genetic variability
OT  - phylogeny
OT  - porcine reproductive and respiratory syndrome virus
OT  - recombination
COIS- The authors declare no conflict of interest.
EDAT- 2023/05/26 19:14
MHDA- 2023/05/26 19:15
PMCR- 2023/04/29
CRDT- 2023/05/26 12:26
PHST- 2023/03/14 00:00 [received]
PHST- 2023/04/18 00:00 [revised]
PHST- 2023/04/28 00:00 [accepted]
PHST- 2023/05/26 19:15 [medline]
PHST- 2023/05/26 19:14 [pubmed]
PHST- 2023/05/26 12:26 [entrez]
PHST- 2023/04/29 00:00 [pmc-release]
AID - vetsci10050325 [pii]
AID - vetsci-10-00325 [pii]
AID - 10.3390/vetsci10050325 [doi]
PST - epublish
SO  - Vet Sci. 2023 Apr 29;10(5):325. doi: 10.3390/vetsci10050325.