PMID- 37235658 OWN - NLM STAT- MEDLINE DCOM- 20230529 LR - 20230615 IS - 2375-2548 (Electronic) IS - 2375-2548 (Linking) VI - 9 IP - 21 DP - 2023 May 26 TI - Transformation of arginine into zero-dimensional nanomaterial endows the material with antibacterial and osteoinductive activity. PG - eadf8645 LID - 10.1126/sciadv.adf8645 [doi] LID - eadf8645 AB - Implant-associated infection is a major threat affecting the success of orthopedic surgeries. Although various materials scavenge bacteria by generating reactive oxygen species (ROS), the intrinsic inability of ROS to distinguish bacteria from cells notably limits the therapeutic effects. Here, we found that the arginine carbon dots (Arg-CDs) that were transformed from arginine exhibited supreme antibacterial and osteoinductive activity. We further designed the Schiff base bond between Arg-CDs and aldehyde hyaluronic acid/gelatin methacryloyl (HG) hydrogel to release Arg-CDs in response to the acidic bone injury microenvironment. The free Arg-CDs could selectively kill bacteria by generating excessive ROS. Furthermore, the Arg-CD-loaded HG composite hydrogel showed excellent osteoinductive activity through inducing the M2 polarization of macrophages by up-regulating interleukin-10 (Il10) expression. Together, our findings revealed that transformation of the arginine into zero-dimensional Arg-CDs could endow the material with exceptional antibacterial and osteoinductive activity, favoring the regeneration of infectious bone. FAU - Li, Jiaying AU - Li J AUID- ORCID: 0000-0002-1930-7403 AD - Orthopedic Institute, Department of Orthopaedic Surgery, The First Affiliated Hospital, State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Suzhou Medical College, Soochow University, Suzhou, Jiangsu 215006, China. FAU - Ma, Jinjin AU - Ma J AUID- ORCID: 0000-0002-3221-2134 AD - Orthopedic Institute, Department of Orthopaedic Surgery, The First Affiliated Hospital, State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Suzhou Medical College, Soochow University, Suzhou, Jiangsu 215006, China. FAU - Sun, Heng AU - Sun H AUID- ORCID: 0000-0001-6515-0710 AD - Orthopedic Institute, Department of Orthopaedic Surgery, The First Affiliated Hospital, State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Suzhou Medical College, Soochow University, Suzhou, Jiangsu 215006, China. FAU - Yu, Meizhe AU - Yu M AD - School of Chemistry and Chemical Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu 210094, China. FAU - Wang, Huan AU - Wang H AUID- ORCID: 0000-0003-0886-4615 AD - Orthopedic Institute, Department of Orthopaedic Surgery, The First Affiliated Hospital, State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Suzhou Medical College, Soochow University, Suzhou, Jiangsu 215006, China. FAU - Meng, Qingchen AU - Meng Q AD - Orthopedic Institute, Department of Orthopaedic Surgery, The First Affiliated Hospital, State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Suzhou Medical College, Soochow University, Suzhou, Jiangsu 215006, China. FAU - Li, Zexi AU - Li Z AD - Orthopedic Institute, Department of Orthopaedic Surgery, The First Affiliated Hospital, State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Suzhou Medical College, Soochow University, Suzhou, Jiangsu 215006, China. FAU - Liu, Dachuan AU - Liu D AUID- ORCID: 0000-0003-3232-4707 AD - Orthopedic Institute, Department of Orthopaedic Surgery, The First Affiliated Hospital, State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Suzhou Medical College, Soochow University, Suzhou, Jiangsu 215006, China. FAU - Bai, Jianzhong AU - Bai J AD - Orthopedic Institute, Department of Orthopaedic Surgery, The First Affiliated Hospital, State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Suzhou Medical College, Soochow University, Suzhou, Jiangsu 215006, China. FAU - Liu, Guoping AU - Liu G AD - Orthopedic Institute, Department of Orthopaedic Surgery, The First Affiliated Hospital, State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Suzhou Medical College, Soochow University, Suzhou, Jiangsu 215006, China. FAU - Xing, Xiaodong AU - Xing X AUID- ORCID: 0000-0002-0671-1132 AD - School of Chemistry and Chemical Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu 210094, China. FAU - Han, Fengxuan AU - Han F AUID- ORCID: 0000-0003-3301-6888 AD - Orthopedic Institute, Department of Orthopaedic Surgery, The First Affiliated Hospital, State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Suzhou Medical College, Soochow University, Suzhou, Jiangsu 215006, China. FAU - Li, Bin AU - Li B AUID- ORCID: 0000-0001-8516-9953 AD - Orthopedic Institute, Department of Orthopaedic Surgery, The First Affiliated Hospital, State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Suzhou Medical College, Soochow University, Suzhou, Jiangsu 215006, China. AD - School of Biology and Basic Medical Sciences, Suzhou Medical College, Soochow University, Suzhou, Jiangsu 215123, China. LA - eng PT - Journal Article DEP - 20230526 PL - United States TA - Sci Adv JT - Science advances JID - 101653440 RN - 0 (Reactive Oxygen Species) RN - 94ZLA3W45F (Arginine) RN - 0 (Anti-Bacterial Agents) RN - 0 (Hydrogels) RN - 7440-44-0 (Carbon) SB - IM MH - Reactive Oxygen Species MH - *Arginine/pharmacology MH - Anti-Bacterial Agents/chemistry MH - Hydrogels/pharmacology/therapeutic use MH - Carbon/chemistry MH - *Nanostructures PMC - PMC10219602 EDAT- 2023/05/26 19:15 MHDA- 2023/05/29 06:42 PMCR- 2023/05/26 CRDT- 2023/05/26 14:03 PHST- 2023/05/29 06:42 [medline] PHST- 2023/05/26 19:15 [pubmed] PHST- 2023/05/26 14:03 [entrez] PHST- 2023/05/26 00:00 [pmc-release] AID - adf8645 [pii] AID - 10.1126/sciadv.adf8645 [doi] PST - ppublish SO - Sci Adv. 2023 May 26;9(21):eadf8645. doi: 10.1126/sciadv.adf8645. Epub 2023 May 26.