PMID- 37237777
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230530
IS  - 2079-6382 (Print)
IS  - 2079-6382 (Electronic)
IS  - 2079-6382 (Linking)
VI  - 12
IP  - 5
DP  - 2023 May 8
TI  - Cellulose Hydrogel with Hyaluronic Acid and Silver Nanoparticles: 
      Sustained-Release Formulation with Antibacterial Properties against Pseudomonas 
      aeruginosa.
LID - 10.3390/antibiotics12050873 [doi]
LID - 873
AB  - Pathogenic bacteria resistant to conventional antibiotics represent a global 
      challenge and justify the need for new antimicrobials capable of combating 
      bacterial multidrug resistance. This study describes the development of a topical 
      hydrogel in a formulation composed of cellulose, hyaluronic acid (HA), and silver 
      nanoparticles (AgNPs) against strains of Pseudomonas aeruginosa. AgNPs as an 
      antimicrobial agent were synthesized by a new method based on green chemistry, 
      using arginine as a reducing agent and potassium hydroxide as a carrier. Scanning 
      electron microscopy showed the formation of a composite between cellulose and HA 
      in a three-dimensional network of cellulose fibrils, with thickening of the 
      fibrils and filling of spaces by HA with the presence of pores. 
      Ultraviolet-visible spectroscopy (UV-vis) and particle size distribution for 
      dynamic light scattering (DLS) confirmed the formation of AgNPs with peak 
      absorption at ~430 nm and 57.88 nm. AgNPs dispersion showed a minimum inhibitory 
      concentration (MIC) of 1.5 microg/mL. The time-kill assay showed that after 3 h of 
      exposure to the hydrogel containing AgNPs, there were no viable cells, 
      corresponding to a bactericidal efficacy of 99.999% in the 95% confidence level. 
      We obtained a hydrogel that is easy to apply, with sustained release and 
      bactericidal properties against strains of Pseudomonas aeruginosa at low 
      concentrations of the agent.
FAU - Sumini, Mirian
AU  - Sumini M
AD  - Department of Microbiology, Biological Sciences Center, Londrina State 
      University, Londrina 86057-970, Parana, Brazil.
FAU - Souza, Clara Ruiz de
AU  - Souza CR
AD  - Department of Pharmacy, Health Sciences Center, Londrina State University, 
      Londrina 86057-970, Parana, Brazil.
FAU - Andrade, Gabriel Jonathan Sousa
AU  - Andrade GJS
AD  - Department of Biochemistry and Biotechnology, Exact Sciences Center, Londrina 
      State University, Londrina 86057-970, Parana, Brazil.
FAU - Oliveira, Igor Roberto Cabral
AU  - Oliveira IRC
AUID- ORCID: 0000-0001-7545-8081
AD  - Department of Civil Engineering, Faculty of Technology, Federal University of 
      Amazonas, Manaus 69077-000, Amazonas, Brazil.
FAU - Scandorieiro, Sara
AU  - Scandorieiro S
AUID- ORCID: 0000-0003-4012-9100
AD  - Department of Microbiology, Biological Sciences Center, Londrina State 
      University, Londrina 86057-970, Parana, Brazil.
FAU - Tischer, Cesar Augusto
AU  - Tischer CA
AD  - Department of Biochemistry and Biotechnology, Exact Sciences Center, Londrina 
      State University, Londrina 86057-970, Parana, Brazil.
FAU - Kobayashi, Renata Katsuko Takayama
AU  - Kobayashi RKT
AUID- ORCID: 0000-0001-7170-2155
AD  - Department of Microbiology, Biological Sciences Center, Londrina State 
      University, Londrina 86057-970, Parana, Brazil.
FAU - Nakazato, Gerson
AU  - Nakazato G
AUID- ORCID: 0000-0001-8624-795X
AD  - Department of Microbiology, Biological Sciences Center, Londrina State 
      University, Londrina 86057-970, Parana, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20230508
PL  - Switzerland
TA  - Antibiotics (Basel)
JT  - Antibiotics (Basel, Switzerland)
JID - 101637404
PMC - PMC10215745
OTO - NOTNLM
OT  - antibacterial properties
OT  - hydrogel topic
OT  - nanocomposite
OT  - pathogenic bacteria
OT  - silver nanoparticles
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of the data; 
      in the writing of the manuscript; or in the decision to publish the results.
EDAT- 2023/05/27 09:42
MHDA- 2023/05/27 09:43
PMCR- 2023/05/08
CRDT- 2023/05/27 01:02
PHST- 2023/03/29 00:00 [received]
PHST- 2023/05/04 00:00 [revised]
PHST- 2023/05/05 00:00 [accepted]
PHST- 2023/05/27 09:43 [medline]
PHST- 2023/05/27 09:42 [pubmed]
PHST- 2023/05/27 01:02 [entrez]
PHST- 2023/05/08 00:00 [pmc-release]
AID - antibiotics12050873 [pii]
AID - antibiotics-12-00873 [pii]
AID - 10.3390/antibiotics12050873 [doi]
PST - epublish
SO  - Antibiotics (Basel). 2023 May 8;12(5):873. doi: 10.3390/antibiotics12050873.