PMID- 37239349
OWN - NLM
STAT- MEDLINE
DCOM- 20230529
LR  - 20230531
IS  - 2073-4425 (Electronic)
IS  - 2073-4425 (Linking)
VI  - 14
IP  - 5
DP  - 2023 Apr 27
TI  - An In Vitro Model of Glioma Development.
LID - 10.3390/genes14050990 [doi]
LID - 990
AB  - Gliomas are the prevalent forms of brain cancer and derive from glial cells. 
      Among them, astrocytomas are the most frequent. Astrocytes are fundamental for 
      most brain functions, as they contribute to neuronal metabolism and 
      neurotransmission. When they acquire cancer properties, their functions are 
      altered, and, in addition, they start invading the brain parenchyma. Thus, a 
      better knowledge of transformed astrocyte molecular properties is essential. With 
      this aim, we previously developed rat astrocyte clones with increasing cancer 
      properties. In this study, we used proteomic analysis to compare the most 
      transformed clone (A-FC6) with normal primary astrocytes. We found that 154 
      proteins are downregulated and 101 upregulated in the clone. Moreover, 46 
      proteins are only expressed in the clone and 82 only in the normal cells. 
      Notably, only 11 upregulated/unique proteins are encoded in the duplicated q arm 
      of isochromosome 8 (i(8q)), which cytogenetically characterizes the clone. Since 
      both normal and transformed brain cells release extracellular vesicles (EVs), 
      which might induce epigenetic modifications in the neighboring cells, we also 
      compared EVs released from transformed and normal astrocytes. Interestingly, we 
      found that the clone releases EVs containing proteins, such as matrix 
      metalloproteinase 3 (MMP3), that can modify the extracellular matrix, thus 
      allowing invasion.
FAU - Schiera, Gabriella
AU  - Schiera G
AUID- ORCID: 0000-0002-8638-6455
AD  - Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, 
      University of Palermo, Viale delle Scienze, Edificio 16, 90128 Palermo, Italy.
FAU - Cancemi, Patrizia
AU  - Cancemi P
AUID- ORCID: 0000-0002-3951-7213
AD  - Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, 
      University of Palermo, Viale delle Scienze, Edificio 16, 90128 Palermo, Italy.
FAU - Di Liegro, Carlo Maria
AU  - Di Liegro CM
AD  - Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, 
      University of Palermo, Viale delle Scienze, Edificio 16, 90128 Palermo, Italy.
FAU - Naselli, Flores
AU  - Naselli F
AUID- ORCID: 0009-0000-8075-0642
AD  - Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, 
      University of Palermo, Viale delle Scienze, Edificio 16, 90128 Palermo, Italy.
FAU - Volpes, Sara
AU  - Volpes S
AD  - Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, 
      University of Palermo, Viale delle Scienze, Edificio 16, 90128 Palermo, Italy.
FAU - Cruciata, Ilenia
AU  - Cruciata I
AUID- ORCID: 0000-0002-6614-7270
AD  - Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, 
      University of Palermo, Viale delle Scienze, Edificio 16, 90128 Palermo, Italy.
FAU - Cardinale, Paola Sofia
AU  - Cardinale PS
AD  - Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, 
      University of Palermo, Viale delle Scienze, Edificio 16, 90128 Palermo, Italy.
FAU - Vaglica, Fabiola
AU  - Vaglica F
AD  - Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, 
      University of Palermo, Viale delle Scienze, Edificio 16, 90128 Palermo, Italy.
FAU - Calligaris, Matteo
AU  - Calligaris M
AD  - Proteomics Group, Department of Research, ISMETT-IRCCS, Ri.MED Foundation, 90127 
      Palermo, Italy.
FAU - Carreca, Anna Paola
AU  - Carreca AP
AUID- ORCID: 0000-0002-7389-7555
AD  - Proteomics Group, Department of Research, ISMETT-IRCCS, Ri.MED Foundation, 90127 
      Palermo, Italy.
FAU - Chiarelli, Roberto
AU  - Chiarelli R
AUID- ORCID: 0000-0001-8381-3389
AD  - Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, 
      University of Palermo, Viale delle Scienze, Edificio 16, 90128 Palermo, Italy.
FAU - Scilabra, Simone Dario
AU  - Scilabra SD
AUID- ORCID: 0000-0001-7300-6153
AD  - Proteomics Group, Department of Research, ISMETT-IRCCS, Ri.MED Foundation, 90127 
      Palermo, Italy.
FAU - Leone, Olga
AU  - Leone O
AD  - Department of Biomedicine, Neurosciences and Advanced Diagnostics, University of 
      Palerm, Via del Vespro, 129, 90127 Palermo, Italy.
FAU - Caradonna, Fabio
AU  - Caradonna F
AUID- ORCID: 0000-0002-7659-7312
AD  - Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, 
      University of Palermo, Viale delle Scienze, Edificio 16, 90128 Palermo, Italy.
FAU - Di Liegro, Italia
AU  - Di Liegro I
AUID- ORCID: 0000-0002-0546-1274
AD  - Department of Biomedicine, Neurosciences and Advanced Diagnostics, University of 
      Palerm, Via del Vespro, 129, 90127 Palermo, Italy.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230427
PL  - Switzerland
TA  - Genes (Basel)
JT  - Genes
JID - 101551097
RN  - 0 (Proteins)
SB  - IM
MH  - Rats
MH  - Animals
MH  - Proteomics
MH  - *Glioma/genetics/metabolism
MH  - *Brain Neoplasms/genetics/metabolism
MH  - Brain/metabolism
MH  - Astrocytes/metabolism
MH  - Proteins/metabolism
PMC - PMC10217752
OTO - NOTNLM
OT  - astrocyte cell lines
OT  - astrocytomas
OT  - chromosome alterations
OT  - epigenetic alterations
OT  - extracellular vesicles (EVs)
OT  - metalloproteinases
OT  - proteomics
COIS- The authors declare no conflict of interest.
EDAT- 2023/05/27 09:42
MHDA- 2023/05/29 06:41
PMCR- 2023/04/27
CRDT- 2023/05/27 01:10
PHST- 2023/03/27 00:00 [received]
PHST- 2023/04/20 00:00 [revised]
PHST- 2023/04/24 00:00 [accepted]
PHST- 2023/05/29 06:41 [medline]
PHST- 2023/05/27 09:42 [pubmed]
PHST- 2023/05/27 01:10 [entrez]
PHST- 2023/04/27 00:00 [pmc-release]
AID - genes14050990 [pii]
AID - genes-14-00990 [pii]
AID - 10.3390/genes14050990 [doi]
PST - epublish
SO  - Genes (Basel). 2023 Apr 27;14(5):990. doi: 10.3390/genes14050990.