PMID- 37243264
OWN - NLM
STAT- MEDLINE
DCOM- 20230529
LR  - 20230530
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 15
IP  - 5
DP  - 2023 May 16
TI  - Super-Resolution Microscopy Analysis of Hepatitis B Viral cccDNA and Host 
      Factors.
LID - 10.3390/v15051178 [doi]
LID - 1178
AB  - Infection with hepatitis B virus (HBV) cannot be cured completely because of the 
      persistence of covalently closed circular DNA (cccDNA). We previously found that 
      the host gene dedicator of cytokinesis 11 (DOCK11) was required for HBV 
      persistence. In this study, we further investigated the mechanism that links 
      DOCK11 to other host genes in the regulation of cccDNA transcription. cccDNA 
      levels were determined by quantitative real-time polymerase chain reaction (qPCR) 
      and fluorescence in situ hybridization (FISH) in stable HBV-producing cell lines 
      and HBV-infected PXB-cells(R). Interactions between DOCK11 and other host genes 
      were identified by super-resolution microscopy, immunoblotting, and chromatin 
      immunoprecipitation. FISH facilitated the subcellular localization of key HBV 
      nucleic acids. Interestingly, although DOCK11 partially colocalized with histone 
      proteins, such as H3K4me3 and H3K27me3, and nonhistone proteins, such as RNA Pol 
      II, it played limited roles in histone modification and RNA transcription. DOCK11 
      was functionally involved in regulating the subnuclear distribution of host 
      factors and/or cccDNA, resulting in an increase in cccDNA closely located to 
      H3K4me3 and RNA Pol II for activating cccDNA transcription. Thus, it was 
      suggested that the association of cccDNA-bound Pol II and H3K4me3 required the 
      assistance of DOCK11. DOCK11 facilitated the association of cccDNA with H3K4me3 
      and RNA Pol II.
FAU - Doan, Phuong Thi Bich
AU  - Doan PTB
AUID- ORCID: 0000-0002-0363-5250
AD  - Department of Gastroenterology, Graduate School of Medicine, Kanazawa University, 
      13-1 Takara-Machi, Kanazawa 920-8641, Japan.
FAU - Nio, Kouki
AU  - Nio K
AUID- ORCID: 0000-0002-0971-8313
AD  - Department of Gastroenterology, Graduate School of Medicine, Kanazawa University, 
      13-1 Takara-Machi, Kanazawa 920-8641, Japan.
FAU - Shimakami, Tetsuro
AU  - Shimakami T
AD  - Department of Gastroenterology, Graduate School of Medicine, Kanazawa University, 
      13-1 Takara-Machi, Kanazawa 920-8641, Japan.
FAU - Kuroki, Kazuyuki
AU  - Kuroki K
AD  - Department of Gastroenterology, Graduate School of Medicine, Kanazawa University, 
      13-1 Takara-Machi, Kanazawa 920-8641, Japan.
FAU - Li, Ying-Yi
AU  - Li YY
AD  - Department of Gastroenterology, Graduate School of Medicine, Kanazawa University, 
      13-1 Takara-Machi, Kanazawa 920-8641, Japan.
FAU - Sugimoto, Saiho
AU  - Sugimoto S
AD  - Department of Gastroenterology, Graduate School of Medicine, Kanazawa University, 
      13-1 Takara-Machi, Kanazawa 920-8641, Japan.
FAU - Takayama, Hideo
AU  - Takayama H
AUID- ORCID: 0000-0001-8247-483X
AD  - Department of Gastroenterology, Graduate School of Medicine, Kanazawa University, 
      13-1 Takara-Machi, Kanazawa 920-8641, Japan.
FAU - Okada, Hikari
AU  - Okada H
AD  - Department of Gastroenterology, Graduate School of Medicine, Kanazawa University, 
      13-1 Takara-Machi, Kanazawa 920-8641, Japan.
FAU - Kaneko, Shuichi
AU  - Kaneko S
AD  - Department of Gastroenterology, Graduate School of Medicine, Kanazawa University, 
      13-1 Takara-Machi, Kanazawa 920-8641, Japan.
FAU - Honda, Masao
AU  - Honda M
AD  - Department of Gastroenterology, Graduate School of Medicine, Kanazawa University, 
      13-1 Takara-Machi, Kanazawa 920-8641, Japan.
FAU - Yamashita, Taro
AU  - Yamashita T
AUID- ORCID: 0000-0002-3383-9131
AD  - Department of Gastroenterology, Graduate School of Medicine, Kanazawa University, 
      13-1 Takara-Machi, Kanazawa 920-8641, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230516
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
RN  - EC 2.7.7.- (RNA Polymerase II)
RN  - 0 (DNA, Viral)
RN  - 0 (DNA, Circular)
SB  - IM
MH  - Humans
MH  - RNA Polymerase II/genetics/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy
MH  - Virus Replication/genetics
MH  - DNA, Viral/genetics/metabolism
MH  - Hepatitis B virus/physiology
MH  - DNA, Circular/genetics/metabolism
MH  - *Hepatitis B/genetics
MH  - *Hepatitis B, Chronic
PMC - PMC10223333
OTO - NOTNLM
OT  - H3K4me3
OT  - HBV
OT  - RNA Pol II
OT  - fluorescence in in situ hybridization FISH
OT  - transcription factors
COIS- The authors declare no conflict of interest.
EDAT- 2023/05/27 09:43
MHDA- 2023/05/29 06:42
PMCR- 2023/05/16
CRDT- 2023/05/27 01:33
PHST- 2023/04/18 00:00 [received]
PHST- 2023/05/13 00:00 [revised]
PHST- 2023/05/15 00:00 [accepted]
PHST- 2023/05/29 06:42 [medline]
PHST- 2023/05/27 09:43 [pubmed]
PHST- 2023/05/27 01:33 [entrez]
PHST- 2023/05/16 00:00 [pmc-release]
AID - v15051178 [pii]
AID - viruses-15-01178 [pii]
AID - 10.3390/v15051178 [doi]
PST - epublish
SO  - Viruses. 2023 May 16;15(5):1178. doi: 10.3390/v15051178.