PMID- 37247596
OWN - NLM
STAT- MEDLINE
DCOM- 20230612
LR  - 20230612
IS  - 1873-7072 (Electronic)
IS  - 0308-8146 (Linking)
VI  - 424
DP  - 2023 Oct 30
TI  - Nutrition, allergenicity and physicochemical qualities of food-grade protein 
      extracts from Nannochloropsis oculata.
PG  - 136459
LID - S0308-8146(23)01077-4 [pii]
LID - 10.1016/j.foodchem.2023.136459 [doi]
AB  - Microalgae offer an opportunity to act as a sustainable source of dietary 
      protein. This study aimed to evaluate the impact of different protein extraction 
      methods on the nutritional and physicochemical properties of Nannochloropsis 
      oculata. Food-grade protein extracts were obtained by hypotonic osmotic shock 
      using milli-Q water. Food grade (FG) and non-food grade (NFG) extraction buffers 
      were compared along with three cell disruption methods including bead beating, 
      probe sonication and a combination of both methods for protein extraction. Mass 
      spectrometry was used for protein and putative allergen identification in FG 
      extracts. Bead beating led to a slightly higher number of identifiable proteins 
      in FG extracts compared to control condition. Putative allergenic proteins were 
      identified in FG extracts of N. oculata using different in-silico methods. These 
      findings support the need to further evaluate the potential allergenic proteins 
      in microalgae including N. oculata such as immunoglobulin E (IgE) binding tests.
CI  - Crown Copyright (c) 2023. Published by Elsevier Ltd. All rights reserved.
FAU - Hamzelou, Sara
AU  - Hamzelou S
AD  - CSIRO Health & Biosecurity, Adelaide, SA 5000, Australia. Electronic address: 
      sara.hamzelou@csiro.au.
FAU - Belobrajdic, Damien
AU  - Belobrajdic D
AD  - CSIRO Health & Biosecurity, Adelaide, SA 5000, Australia.
FAU - Juhasz, Angela
AU  - Juhasz A
AD  - Australian Research Council Centre of Excellence for Innovations in Peptide and 
      Protein Science, School of Science, Edith Cowan University, Joondalup, WA 6027, 
      Australia.
FAU - Brook, Henri
AU  - Brook H
AD  - CSIRO Health & Biosecurity, Adelaide, SA 5000, Australia.
FAU - Bose, Utpal
AU  - Bose U
AD  - Australian Research Council Centre of Excellence for Innovations in Peptide and 
      Protein Science, School of Science, Edith Cowan University, Joondalup, WA 6027, 
      Australia; CSIRO Agriculture and Food, St Lucia, QLD 4067, Australia.
FAU - Colgrave, Michelle L
AU  - Colgrave ML
AD  - Australian Research Council Centre of Excellence for Innovations in Peptide and 
      Protein Science, School of Science, Edith Cowan University, Joondalup, WA 6027, 
      Australia; CSIRO Agriculture and Food, St Lucia, QLD 4067, Australia.
FAU - Broadbent, James A
AU  - Broadbent JA
AD  - CSIRO Agriculture and Food, St Lucia, QLD 4067, Australia.
LA  - eng
PT  - Journal Article
DEP - 20230524
PL  - England
TA  - Food Chem
JT  - Food chemistry
JID - 7702639
RN  - 0 (Allergens)
SB  - IM
MH  - Allergens/chemistry
MH  - Food
MH  - *Stramenopiles/chemistry
MH  - *Microalgae/chemistry
OTO - NOTNLM
OT  - Allergens
OT  - Cross-reactivity
OT  - Diet
OT  - Microalgae
OT  - Nannochloropsis oculata
OT  - Protein
OT  - Proteomics
OT  - SWATH-MS
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/05/30 01:06
MHDA- 2023/06/12 06:43
CRDT- 2023/05/29 18:07
PHST- 2023/02/06 00:00 [received]
PHST- 2023/05/11 00:00 [revised]
PHST- 2023/05/22 00:00 [accepted]
PHST- 2023/06/12 06:43 [medline]
PHST- 2023/05/30 01:06 [pubmed]
PHST- 2023/05/29 18:07 [entrez]
AID - S0308-8146(23)01077-4 [pii]
AID - 10.1016/j.foodchem.2023.136459 [doi]
PST - ppublish
SO  - Food Chem. 2023 Oct 30;424:136459. doi: 10.1016/j.foodchem.2023.136459. Epub 2023 
      May 24.