PMID- 37247860
OWN - NLM
STAT- MEDLINE
DCOM- 20230607
LR  - 20231031
IS  - 1000-0607 (Print)
IS  - 1000-0607 (Linking)
VI  - 48
IP  - 5
DP  - 2023 May 25
TI  - [Effects of electroacupuncture on N-methyl-D-aspartate receptor and mitogen 
      activated protein kinase in spinal cord of rats with primary dysmenorrhea].
PG  - 469-74
LID - 10.13702/j.1000-0607.20220584 [doi]
AB  - OBJECTIVE: To observe the effects of electroacupuncture (EA) on the expression 
      levels of N-methyl-D-aspartate receptor (NMDAR), extracellular signal-regulated 
      kinase (ERK)1/2, p38 mitogen activated protein kinase (p38 MAPK) and c-Jun 
      N-terminal kinase (JNK) in the spinal cord of rats with primary dysmenoramia 
      (PDM), so as to explore the underlying mechanism of EA treating PDM. METHODS: 
      Thirty female SD rats were randomly divided into normal group, model group and EA 
      group, with 10 rats in each group. The PDM rat model was established by 
      subcutaneous injection of estradiol benzoate and oxytocin into the thigh. At the 
      same time of modeling, rats in the EA group were treated with EA (50 Hz) at 
      "Sanyinjiao" (SP36) and "Guanyuan" (CV4) once daily, 20 min each time, for 10 
      consecutive days. The writhing times, writhing score and writhing latency were 
      observed within 30 min after oxytocin injection. The uterine pathological 
      morphology was observed by HE staining, and pathological score was calculated. 
      Serum prostaglandin F2alpha (PGF2alpha) and prostaglandin E2 (PGE2) were determined by 
      ELISA. The protein expression levels of NMDAR, ERK1/2, p38MAPK and JNK in spinal 
      cord were detected by Western blot. RESULTS: Compared with the normal group, the 
      writhing times and writhing score were significantly increased (P<0.05); the 
      endometrial epithelial cells showed vacuolar degeneration, death and hyperemia, 
      the uterine pathological score was increased (P<0.05); the content of serum PGF2alpha 
      and the ratio of PGF2alpha/PGE2 were significantly increased (P<0.01), while the 
      content of serum PGE2 was significantly decreased (P<0.01); the expression levels 
      of NMDAR, ERK1/2, p38MAPK and JNK in spinal cord were significantly increased 
      (P<0.05, P<0.01) in the model group. Compared with the model group, the writhing 
      times and writhing score were significantly decreased (P<0.05), the writhing 
      latency was prolonged (P<0.05); the endometrial epithelial cells still showed 
      vacuolar degeneration, death and hyperemia, and the uterine pathological score 
      was decreased (P<0.01); the content of serum PGF2alpha and the ratio of PGF2alpha/PGE2 
      were significantly decreased (P<0.01), while the content of serum PGE2 was 
      significantly increased (P<0.01); the protein expression levels of ERK1/2 and JNK 
      in spinal cord were significantly decreased (P<0.01) in the EA group. CONCLUSION: 
      EA intervention at SP36 and GV4 has obvious analgesic effect on PDM rats, and its 
      mechanisms may be related to reducing serum prostaglandin, alleviating uterine 
      inflammation, and inhibiting the protein expressions of NMDAR, ERK1/2, p38 MAPK 
      and JNK in spinal cord.
FAU - Yuan, Han-Yu
AU  - Yuan HY
AD  - College of Acupuncture-Tuina and Rehabilitation, Hunan University of Chinese 
      Medicine, Changsha 410208, China.
FAU - Li, Juan
AU  - Li J
AD  - College of Acupuncture-Tuina and Rehabilitation, Hunan University of Chinese 
      Medicine, Changsha 410208, China.
FAU - Wang, Shao-Hua
AU  - Wang SH
AD  - College of Acupuncture-Tuina and Rehabilitation, Hunan University of Chinese 
      Medicine, Changsha 410208, China.
FAU - Xue, Xiao
AU  - Xue X
AD  - College of Acupuncture-Tuina and Rehabilitation, Hunan University of Chinese 
      Medicine, Changsha 410208, China.
FAU - Pan, Si-An
AU  - Pan SA
AD  - College of Acupuncture-Tuina and Rehabilitation, Hunan University of Chinese 
      Medicine, Changsha 410208, China.
FAU - Liu, Yu
AU  - Liu Y
AD  - College of Acupuncture-Tuina and Rehabilitation, Hunan University of Chinese 
      Medicine, Changsha 410208, China.
FAU - Yue, Zeng-Hui
AU  - Yue ZH
AD  - College of Acupuncture-Tuina and Rehabilitation, Hunan University of Chinese 
      Medicine, Changsha 410208, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhen Ci Yan Jiu
JT  - Zhen ci yan jiu = Acupuncture research
JID - 8507710
RN  - B7IN85G1HY (Dinoprost)
RN  - K7Q1JQR04M (Dinoprostone)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - 50-56-6 (Oxytocin)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
SB  - IM
MH  - Animals
MH  - Female
MH  - Rats
MH  - Acupuncture Points
MH  - Dinoprost
MH  - Dinoprostone
MH  - Dysmenorrhea/therapy
MH  - *Electroacupuncture
MH  - *Hyperemia
MH  - Mitogen-Activated Protein Kinases
MH  - Oxytocin
MH  - p38 Mitogen-Activated Protein Kinases
MH  - Rats, Sprague-Dawley
MH  - Receptors, N-Methyl-D-Aspartate/genetics
MH  - Spinal Cord
OTO - NOTNLM
OT  - Electroacupuncture
OT  - Extracellular signal-regulated kinase 1/2
OT  - N-methyl-D-aspartic acid receptor
OT  - Primary dysmenorrhea
OT  - c-Jun N-terminal kinase
OT  - p38 mitogen activated protein kinase
EDAT- 2023/05/30 01:06
MHDA- 2023/05/31 06:42
CRDT- 2023/05/29 20:33
PHST- 2023/05/31 06:42 [medline]
PHST- 2023/05/30 01:06 [pubmed]
PHST- 2023/05/29 20:33 [entrez]
AID - 10.13702/j.1000-0607.20220584 [doi]
PST - ppublish
SO  - Zhen Ci Yan Jiu. 2023 May 25;48(5):469-74. doi: 10.13702/j.1000-0607.20220584.