PMID- 37248002
OWN - NLM
STAT- MEDLINE
DCOM- 20230531
LR  - 20230531
IS  - 1095-9939 (Electronic)
IS  - 0048-3575 (Linking)
VI  - 193
DP  - 2023 Jun
TI  - Chlorbenzuron caused growth arrest through interference of glycolysis and energy 
      metabolism in Hyphantria cunea (Lepidoptera: Erebidae) larvae.
PG  - 105466
LID - S0048-3575(23)00131-1 [pii]
LID - 10.1016/j.pestbp.2023.105466 [doi]
AB  - Chlorbenzuron is a kind of benzoylphenylureas (BPUs), which plays a broad role in 
      insect growth regulators (IGRs), with an inhibitory effect on chitin 
      biosynthesis. However, BPUs how to regulate glycolysis and insect growth remains 
      largely unclear. Here, we investigated the effects of chlorbenzuron on growth, 
      nutritional indices, glycolysis, and carbohydrate homeostasis in Hyphantria 
      cunea, a destructive and highly polyphagous forest pest, to elucidate the action 
      mechanism of chlorbenzuron from the perspective of energy metabolism. The results 
      showed that chlorbenzuron dramatically restrained the growth and nutritional 
      indices of H. cunea larvae and resulted in lethality. Meanwhile, we confirmed 
      that chlorbenzuron significantly decreased carbohydrate levels, adenosine 
      triphosphate (ATP), and pyruvic acid (PA) in H. cunea larvae. Further studies 
      indicated that chlorbenzuron caused a significant enhancement in the enzyme 
      activities and mRNA expressions of hexokinase (HK), phosphofructokinase (PFK), 
      and pyruvate kinase (PK), resulting in increased glycolytic flux. Expressions of 
      genes involved in the AMP-activated protein kinase (AMPK) signaling pathway were 
      also upregulated. Moreover, chlorbenzuron had remarkable impacts on H. cunea 
      larvae from the perspective of metabolite enrichment, including the tricarboxylic 
      acid (TCA) cycle and glycolysis, indicating an energy metabolism disorder in 
      larvae. The findings provide a novel insight into the molecular mechanism by 
      which chlorbenzuron abnormally promotes glycolysis and eventually interferes with 
      insect growth and nutritional indices.
CI  - Copyright (c) 2023 Elsevier Inc. All rights reserved.
FAU - Zhao, Yuecheng
AU  - Zhao Y
AD  - School of Forestry, Northeast Forestry University, Harbin 150040, PR China.
FAU - Zou, Chuanshan
AU  - Zou C
AD  - School of Forestry, Northeast Forestry University, Harbin 150040, PR China.
FAU - Zhang, Lu
AU  - Zhang L
AD  - School of Forestry, Northeast Forestry University, Harbin 150040, PR China.
FAU - Li, Chengde
AU  - Li C
AD  - School of Forestry, Northeast Forestry University, Harbin 150040, PR China.
FAU - Li, Xingpeng
AU  - Li X
AD  - School of Forestry, Beihua University, Jilin 132013, PR China. Electronic 
      address: xingpengli@beihua.edu.cn.
FAU - Song, Liwen
AU  - Song L
AD  - Jilin Provincial Academy of Forestry Sciences, Changchun 130033, PR China. 
      Electronic address: lwsong75@126.com.
LA  - eng
PT  - Journal Article
DEP - 20230516
PL  - United States
TA  - Pestic Biochem Physiol
JT  - Pesticide biochemistry and physiology
JID - 1301573
RN  - 0 (chlorbenzuron)
RN  - 0 (Carbohydrates)
SB  - IM
MH  - Animals
MH  - Larva/genetics
MH  - *Moths/genetics
MH  - Energy Metabolism
MH  - Glycolysis
MH  - Carbohydrates
OTO - NOTNLM
OT  - AMPK
OT  - Chlorbenzuron
OT  - Glycolysis
OT  - Hyphantria cunea
OT  - Nutritional indices
COIS- Declaration of Competing Interest None.
EDAT- 2023/05/30 01:06
MHDA- 2023/05/31 06:42
CRDT- 2023/05/29 20:54
PHST- 2023/01/12 00:00 [received]
PHST- 2023/04/26 00:00 [revised]
PHST- 2023/05/14 00:00 [accepted]
PHST- 2023/05/31 06:42 [medline]
PHST- 2023/05/30 01:06 [pubmed]
PHST- 2023/05/29 20:54 [entrez]
AID - S0048-3575(23)00131-1 [pii]
AID - 10.1016/j.pestbp.2023.105466 [doi]
PST - ppublish
SO  - Pestic Biochem Physiol. 2023 Jun;193:105466. doi: 10.1016/j.pestbp.2023.105466. 
      Epub 2023 May 16.