PMID- 37248534
OWN - NLM
STAT- MEDLINE
DCOM- 20230531
LR  - 20230623
IS  - 1478-811X (Electronic)
IS  - 1478-811X (Linking)
VI  - 21
IP  - 1
DP  - 2023 May 29
TI  - Vitamin D receptor (VDR) on the cell membrane of mouse macrophages participates 
      in the formation of lipopolysaccharide tolerance: mVDR is related to the effect 
      of artesunate to reverse LPS tolerance.
PG  - 124
LID - 10.1186/s12964-023-01137-w [doi]
LID - 124
AB  - It is unclear whether membrane vitamin D receptor (mVDR) exists on the macrophage 
      membrane or whether mVDR is associated with lipopolysaccharide (LPS) tolerance. 
      Herein, we report that interfering with caveolae and caveolae-dependent lipid 
      rafts inhibited the formation of LPS tolerance. VDR was detected as co-localized 
      with membrane molecular markers. VDR was detected on the cell membrane and its 
      level was higher in LPS-tolerant cells than that in only LPS treatment cells. 
      Anti-VDR antibodies could abolish the effect of artesunate (AS) to reverse LPS 
      tolerance, and the wild-type peptides (H397 and H305) of VDR, but not the mutant 
      peptide (H397D and H305A), led to the loss of AS's effect. AS decreased the mVDR 
      level in LPS-tolerant cells. In vivo, AS significantly reduced VDR level in the 
      lung tissue of LPS-tolerant mice. In summary, mVDR exists on the cell membrane of 
      macrophages and is closely associated with the formation of LPS tolerance and the 
      effects of AS. Video Abstract.
CI  - (c) 2023. The Author(s).
FAU - Zhang, Yu
AU  - Zhang Y
AD  - Key Laboratory of Basic Pharmacology of Ministry of Education and Joint 
      International Research Laboratory of Ethnomedicine of Ministry of Education, 
      Zunyi Medical University, Zunyi, Guizhou, 563000, China.
FAU - Zhou, Jun
AU  - Zhou J
AD  - Key Laboratory of Basic Pharmacology of Ministry of Education and Joint 
      International Research Laboratory of Ethnomedicine of Ministry of Education, 
      Zunyi Medical University, Zunyi, Guizhou, 563000, China.
FAU - Hua, Ling
AU  - Hua L
AD  - Key Laboratory of Basic Pharmacology of Ministry of Education and Joint 
      International Research Laboratory of Ethnomedicine of Ministry of Education, 
      Zunyi Medical University, Zunyi, Guizhou, 563000, China.
FAU - Li, Pan
AU  - Li P
AD  - Department of Pharmacology, College of Pharmacy, Army Medical University (Third 
      Military Medical University), Chongqing, 400038, PR China.
FAU - Wu, Jiaqi
AU  - Wu J
AD  - Medical Research Centre, Southwest Hospital, Third Military Medical University 
      (Army Medical University), Chongqing, 400038, PR China.
FAU - Shang, Shenglan
AU  - Shang S
AD  - Medical Research Centre, Southwest Hospital, Third Military Medical University 
      (Army Medical University), Chongqing, 400038, PR China.
FAU - Deng, Fei
AU  - Deng F
AD  - Key Laboratory of Basic Pharmacology of Ministry of Education and Joint 
      International Research Laboratory of Ethnomedicine of Ministry of Education, 
      Zunyi Medical University, Zunyi, Guizhou, 563000, China.
FAU - Luo, Jing
AU  - Luo J
AD  - Key Laboratory of Basic Pharmacology of Ministry of Education and Joint 
      International Research Laboratory of Ethnomedicine of Ministry of Education, 
      Zunyi Medical University, Zunyi, Guizhou, 563000, China.
FAU - Liao, Mengling
AU  - Liao M
AD  - Key Laboratory of Basic Pharmacology of Ministry of Education and Joint 
      International Research Laboratory of Ethnomedicine of Ministry of Education, 
      Zunyi Medical University, Zunyi, Guizhou, 563000, China.
FAU - Wang, Nuoyan
AU  - Wang N
AD  - Key Laboratory of Basic Pharmacology of Ministry of Education and Joint 
      International Research Laboratory of Ethnomedicine of Ministry of Education, 
      Zunyi Medical University, Zunyi, Guizhou, 563000, China.
FAU - Pan, Xichun
AU  - Pan X
AD  - Department of Pharmacology, College of Pharmacy, Army Medical University (Third 
      Military Medical University), Chongqing, 400038, PR China.
FAU - Yuan, Yue
AU  - Yuan Y
AD  - Key Laboratory of Basic Pharmacology of Ministry of Education and Joint 
      International Research Laboratory of Ethnomedicine of Ministry of Education, 
      Zunyi Medical University, Zunyi, Guizhou, 563000, China.
FAU - Zheng, Yue
AU  - Zheng Y
AD  - Medical Research Centre, Southwest Hospital, Third Military Medical University 
      (Army Medical University), Chongqing, 400038, PR China.
FAU - Lu, Yonglin
AU  - Lu Y
AD  - Medical Research Centre, Southwest Hospital, Third Military Medical University 
      (Army Medical University), Chongqing, 400038, PR China.
FAU - Huang, Yasi
AU  - Huang Y
AD  - Key Laboratory of Basic Pharmacology of Ministry of Education and Joint 
      International Research Laboratory of Ethnomedicine of Ministry of Education, 
      Zunyi Medical University, Zunyi, Guizhou, 563000, China.
FAU - Zheng, Jiang
AU  - Zheng J
AD  - Medical Research Centre, Southwest Hospital, Third Military Medical University 
      (Army Medical University), Chongqing, 400038, PR China.
FAU - Liu, Xin
AU  - Liu X
AD  - Medical Research Centre, Southwest Hospital, Third Military Medical University 
      (Army Medical University), Chongqing, 400038, PR China. triplestars@163.com.
FAU - Li, Xiaoli
AU  - Li X
AD  - Department of Pharmacology, College of Pharmacy, Chongqing Medical University, 
      Chongqing, 400016, PR China. plum181181@126.com.
AD  - Chongqing Key Laboratory of Drug Metabolism, Chongqing, 400016, China. 
      plum181181@126.com.
FAU - Zhou, Hong
AU  - Zhou H
AD  - Key Laboratory of Basic Pharmacology of Ministry of Education and Joint 
      International Research Laboratory of Ethnomedicine of Ministry of Education, 
      Zunyi Medical University, Zunyi, Guizhou, 563000, China. zhouh64@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Video-Audio Media
DEP - 20230529
PL  - England
TA  - Cell Commun Signal
JT  - Cell communication and signaling : CCS
JID - 101170464
RN  - 0 (Receptors, Calcitriol)
RN  - 0 (Lipopolysaccharides)
RN  - 60W3249T9M (Artesunate)
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Receptors, Calcitriol/metabolism
MH  - *Lipopolysaccharides/pharmacology
MH  - Artesunate/pharmacology
MH  - Cell Membrane/metabolism
MH  - Macrophages/metabolism
PMC - PMC10227983
OTO - NOTNLM
OT  - Artesunate
OT  - Caveolin-1
OT  - LPS tolerance
OT  - Lipid raft
OT  - Membrane vitamin D receptor
COIS- The authors declare no competing interests.
EDAT- 2023/05/30 01:06
MHDA- 2023/05/31 06:42
PMCR- 2023/05/29
CRDT- 2023/05/29 23:33
PHST- 2022/12/03 00:00 [received]
PHST- 2023/04/22 00:00 [accepted]
PHST- 2023/05/31 06:42 [medline]
PHST- 2023/05/30 01:06 [pubmed]
PHST- 2023/05/29 23:33 [entrez]
PHST- 2023/05/29 00:00 [pmc-release]
AID - 10.1186/s12964-023-01137-w [pii]
AID - 1137 [pii]
AID - 10.1186/s12964-023-01137-w [doi]
PST - epublish
SO  - Cell Commun Signal. 2023 May 29;21(1):124. doi: 10.1186/s12964-023-01137-w.